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1.
Figure 1

Figure 1. Growth curves of S. cerevisiae BY4741 parental strain and fps1Δ deletion mutant under ethanol stress.. From: Quantitative 1H-NMR-Metabolomics Reveals Extensive Metabolic Reprogramming and the Effect of the Aquaglyceroporin FPS1 in Ethanol-Stressed Yeast Cells.

(A) Comparison of the growth curves of S. cerevisiae BY4741 in the absence (▪) or presence of 2% v/v (□), 4% v/v (Δ) and 6% v/v (○) of ethanol. (B) Comparison of the growth curves of S. cerevisiae BY4741 (▪, □) and the corresponding deletion mutant fps1Δ (♦, ◊) in the absence (closed symbols) or presence of 2% v/v of ethanol (open symbols).

Artur B. Lourenço, et al. PLoS One. 2013;8(2):e55439.
2.
Figure 6

Figure 6. Integrative overview of the metabolic changes occurring under ethanol stress or upon FPS1 deletion.. From: Quantitative 1H-NMR-Metabolomics Reveals Extensive Metabolic Reprogramming and the Effect of the Aquaglyceroporin FPS1 in Ethanol-Stressed Yeast Cells.

Changes found to occur (as indicated by the black or white arrows) or not (□,▪) in yeast cells growing in the presence of inhibitory concentrations of ethanol (closed symbols), or upon FPS1 deletion (open symbols) are shown. The metabolic scheme was based on information gathered in the KEGG PATHWAY Database (http://www.genome.jp/kegg/pathway.html) and in Yeast Biochemical Pathway Database (http://pathway.yeastgenome.org/).

Artur B. Lourenço, et al. PLoS One. 2013;8(2):e55439.
3.
Figure 5

Figure 5. Comparison between the exo-metabolome of BY4741 parental strain and of fps1Δ deletion mutant.. From: Quantitative 1H-NMR-Metabolomics Reveals Extensive Metabolic Reprogramming and the Effect of the Aquaglyceroporin FPS1 in Ethanol-Stressed Yeast Cells.

Samples were collected during the exponential phase of growth (OD600 nm = 1.0) in the same basal medium supplemented with 0 or 2% v/v of ethanol. (A) 2D score plot displaying the space formed by the two first principal components (R2X for PC1 equal to 0.375 and R2X for PC2 equal to 0.284), (B) dendrogram based on the PCA scores (ward clustering distance measure) considering all principal components of the model and (C) relative abundances of different metabolites in the exo-metabolome of the parental strain and the fps1Δ deletion mutant during the exponential growth phase (OD600 nm = 1.0) in the presence of different ethanol concentrations (0 and 2% v/v). Experimental values are means of four independent experiments with standard deviation error bars.

Artur B. Lourenço, et al. PLoS One. 2013;8(2):e55439.
4.
Figure 3

Figure 3. Exo-metabolome analysis of S. cerevisiae BY4741 parental strain under ethanol stress.. From: Quantitative 1H-NMR-Metabolomics Reveals Extensive Metabolic Reprogramming and the Effect of the Aquaglyceroporin FPS1 in Ethanol-Stressed Yeast Cells.

Samples were collected during the exponential phase of growth (OD600 nm = 1.0) in the same basal medium supplemented with different ethanol concentrations (% v/v). (A) 2D score plot displaying the space formed by the two first principal components (R2X for PC1 equal to 0.522 and R2X for PC2 equal to 0.166), (B) 2D loading plot displaying the space formed by the two first principal components and presenting the characteristic bins for a set of metabolites and (C) relative abundance of different metabolites in the exo-metabolome of S. cerevisiae BY4741 cells during the exponential phase of growth (OD600 nm = 1.0) in the presence of different ethanol concentrations (0, 2, 4 and 6% v/v). Experimental values are means of four independent experiments with standard deviation error bars. Key: 1 Orotic acid, 2 Succininc acid, 3 2-Oxoglutaric acid, 4 L-leucine, 5 L-methionine, 6 Glucose, 7 L-histidine, 8 Glycerol, 9 Formic acid, 10 Uracil, 11 Acetic acid, 12 Pyruvic acid, 13 Acetoin, 14 Acetaldehyde, 15 Lactic acid, 16 L-alanine and 17 Fumaric acid.

Artur B. Lourenço, et al. PLoS One. 2013;8(2):e55439.
5.
Figure 4

Figure 4. Comparison between the endo-metabolome of BY4741 parental strain and of fps1Δ deletion mutant.. From: Quantitative 1H-NMR-Metabolomics Reveals Extensive Metabolic Reprogramming and the Effect of the Aquaglyceroporin FPS1 in Ethanol-Stressed Yeast Cells.

Yeast cells were harvested during the exponential phase of growth (OD600 nm = 1.0) in the same basal medium supplemented with 0 or 2% v/v of ethanol. (A) 2D score plot displaying the space formed by the two first principal components (R2X for PC1 equal to 0.525 and R2X for PC2 equal to 0.321), (B) 2D loading plot displaying the space formed by the two first principal components (with characteristic bins for a set of metabolites) and (C) relative abundances of different metabolites in the endo-metabolome of the parental strain and the fps1Δ deletion strain during the exponential growth phase (OD600 nm = 1.0) in the presence of different ethanol concentrations (0 and 2% v/v). Experimental values are means of six independent experiments with standard deviation error bars. Key: 1 NAD+, 2 NADP+, 3 L-tyrosine, 4 Fumaric acid, 5 L-phenylalanine, 6 Citric acid, 7 Succininic acid, 8 Glycerol, 9 L-glutamic acid, 10 L-leucine, 11 L-methionine, 12 L-alanine, 13 L-ornithine, 14 L-threonine, 15 L-valine,16 L-glutamine, 17 L-aspartic acid, 18 L-malic acid, 19 L-histidine, 20 L-serine, 21 L-asparagine, 22 L-lysine, 23 Thiamine, 24 NADH and 25 L-proline.

Artur B. Lourenço, et al. PLoS One. 2013;8(2):e55439.
6.
Figure 2

Figure 2. Endo-metabolome analysis of S. cerevisiae BY4741 parental strain under ethanol stress.. From: Quantitative 1H-NMR-Metabolomics Reveals Extensive Metabolic Reprogramming and the Effect of the Aquaglyceroporin FPS1 in Ethanol-Stressed Yeast Cells.

Yeast cells were harvested during the exponential phase of growth (OD600 nm = 1.0) in the same basal medium and supplemented with different ethanol concentrations (% v/v). (A) 2D score plot displaying the space formed by the two first principal components (R2X for PC1 equal to 0.792 and R2X for PC2 equal to 0.129), (B) 2D loading plot displaying the space formed by the two first principal components and presenting the characteristic bins for a set of metabolites and (C) Relative abundance of different metabolites in the endo-metabolome of S. cerevisiae BY4741 cells harvested during the exponential phase of growth (OD600 nm = 1.0) in the presence of different ethanol concentrations (0, 2, 4 and 6% v/v). Each plot displays the variation in the relative abundance of a representative bin in the spectrum for each amino acid. Experimental values are means of six independent experiments with standard deviation error bars. Key: 1 Glycerol, 2 L-arginine, 3 NAD+, 4 NADP+, 5 L-tyrosine, 6 L-histidine, 7 Trehalose, 8 NADH, 9 Fumaric acid, 10 L-proline, 11 L-phenylalanine, 12 L-asparagine, 13 Citric acid, 14 L-serine, 15 Succininic acid, 16 L-aspartic acid, 17 Malic acid, 18 L-glutamine, 19 L-alanine, 20 L-lysine, 21 L-glycine, 22 L-threonine, 23 L-valine, 24 L-methionine, 25 L-leucine, 26 L-glutamic acid and 27 L-ornithine.

Artur B. Lourenço, et al. PLoS One. 2013;8(2):e55439.

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