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1.
Figure 2

Figure 2. From: A prevalent alpha-proteobacterium Paracoccus sp. in a population of the Cayenne ticks (Amblyomma cajennense) from Rio de Janeiro, Brazil.

Agarose gel of 16S rDNA PCR products digested with HinfI. PCR reactions were achieved using the Rickettsia spp. specific primer set, as described in the text. Lanes include amplicon digestions for Rickettsia sp. A45 (Lane 1) and Paracoccus sp-Cayenne (Lane 2), as well as the tick-total DNA samples Cav22F and Cav49F (Lanes 3–4). The non-digested PCR product for Cav49F was run in parallel (Lane 5). The molecular marker is presented in base pairs (bp). The expected bands for the Rickettsia sample include the 195 bp, 117 bp and 124 bp bands, the last two appearing as a single band in the gels. The Paracoccus sp-Cayenne expected profile includes the bands of 297 bp and 100 bp, as well as the faint band of 25 bp, not observed in the gels.

Erik Machado-Ferreira, et al. Genet Mol Biol. 2012 December;35(4):862-867.
2.
Figure 1

Figure 1. From: A prevalent alpha-proteobacterium Paracoccus sp. in a population of the Cayenne ticks (Amblyomma cajennense) from Rio de Janeiro, Brazil.

Phylogenetic inferences by Neighbour-Joining (NJ) with Kimura 2-parameter. GenBank accession numbers are presented in parenthesis. A) 16S rDNA gene sequence alignment of Paracoccus sp-Cayenne and Rickettsia sp. A45, P. yeeii I (AY014178), P. yeeii II (DQ857285), P. yeeii III (AY014179), P. yeeii IV (AY014179), P. carotinifaciens (AB006899), P. marcusii I (DQ298023), P. marcusii II (DQ298022), Paracoccus sp. SW-3 (FJ593906), P. seriniphilus (AJ428275), P. pantotrophus (AB098590), P. denitrificans (CP000490), P. marinus (AB681212), P. sulforoxidans (JQ291588), P. alcaliphilus (AJ294415), Paracoccus SA5 (AY864654), Paracoccus sp. JLT1289 (EU650196), P. homiensis (NR_043733), Paracoccus sp. RA19 (AJ507806), R. peacockii (DQ062433), R. honei (U17645), R. rickettsii (U11021), R. raoultii (DQ365809), R. amblyommii (U11012), R. felis (CP000053), R. typhi (AE017197) and E. coli (AY84014). B) htrA gene sequence alignment of Rickettsia sp. A45, R. rickettsii (DQ176856), R. peacockii (AF260571), R. honei (AF060704), R. raoultii (EF392727), R. amblyommii I (DQ517291), R. amblyommii II (AY375162), R. felis (CP000053) and R. typhi (AE017197). C) ompA sequence aligment of Rickettsia sp. A45, R. amblyommii I (AY062007), R. amblyommii II (EF194096), R. raoultii (DQ365799), R. honei (AF018075) and R. rickettsii (DQ002504). Sequences were aligned using the CLUSTAL W 2.0 program, and phylogenetic inferences obtained using MEGA 5 software. Internal node supports were calculated using bootstrap with 1000 replicas. Bootstrap values below 80% are not presented.

Erik Machado-Ferreira, et al. Genet Mol Biol. 2012 December;35(4):862-867.

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