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Results: 3

1.
Figure 1

Figure 1. Effects of serum levels of IL17 and tumor size on HCC early recurrence (A,B,C) and overall survival of the patients (D,E,F).. From: Elevated Pretherapy Serum IL17 in Primary Hepatocellular Carcinoma Patients Correlate to Increased Risk of Early Recurrence after Curative Hepatectomy.

Kaplan-Meier estimate on HCC recurrence based on elevated serum levels of IL17 alone (A), on tumor size alone (B), and on combination of the two factors (C). Kaplan-Meier estimate patient overall survival based on elevated serum levels of IL17 alone (D), on tumor size alone (E), and on combination of the two factors (F).

Jianxiong Wu, et al. PLoS One. 2012;7(12):e50035.
2.
Figure 2

Figure 2. Intracellular IL17 staining in PBMCs and IHL of HCC patients.. From: Elevated Pretherapy Serum IL17 in Primary Hepatocellular Carcinoma Patients Correlate to Increased Risk of Early Recurrence after Curative Hepatectomy.

A: IL17 staining in PBMCs or IHL isolated from one representative of 9 independent HCC patients stimulated with anti-human-CD3 and CD28 (CD3+CD28). As parallel, IHL in 3 of the HCC patients were stimulated with PMA and Ionomycin (PMA+ION) were used to confirm the presence of IL17-producing T cells. Analysis was based on CD3+ gating (gating strategy is provided in Figure S2). B: Percentage of IL-17+ cells in the indicated cell populations. Each dot represents one of HCC patient. PBMCs: peripheral blood monoculear cells; IHL: intrahepatic lymphocytes. PMA: phorbol 12-myristate 13-acetate, ION: Ionomycin. **indicates P<0.001.

Jianxiong Wu, et al. PLoS One. 2012;7(12):e50035.
3.
Figure 3

Figure 3. Proliferation of hepatocellular carcinoma cells in presence of activated IL17-producing T cells.. From: Elevated Pretherapy Serum IL17 in Primary Hepatocellular Carcinoma Patients Correlate to Increased Risk of Early Recurrence after Curative Hepatectomy.

A: Diagram of the co-culture experiments. B, C: The proliferation of HCC cells, QGY-7703 or Hep3B cell lines, was determined using a CCK8 reagents after being co-cultured with IL17-producing T cells for 48 h. PBMCs were isolated from the HCC patients and cultured with recombinant human IL-23 in the presence of plate-bound anti-human CD3 and anti-human CD28 for 7–10 days. IL17 production was confirmed by intracellular staining (Figure S3). The proliferation of HCC cells (ctrl) containing anti-human CD3 and anti-CD28 antibodies in the medium but without adding cultured T cells in the upper chambers was used as control. All the antibodies used here were 5 µg/ml. Letter “c” represents isotype control. The upper chambers contained T cells were removed before CCK8 reagents were added. Triplicates were performed for each of the treatment. *: P<0.05. Data shown is representative one from 3 independent experiments.

Jianxiong Wu, et al. PLoS One. 2012;7(12):e50035.

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