Results: 4

1.
Figure 3

Figure 3. From: A Quantitative Analysis of the Fate of Au Nanocages after Uptake by U87-MG Tumor Cells under Both in vitro and in vivo Conditions.

a–d) Time-lapse photographs showing a tumor growing on the dorsal side of a nude mouse after subcutaneous injection of U87-MG cells containing the AuNCs. The tumor was non-invasively monitored using PAM up to 6 days post injection. e–l) Time-lapse in vivo PAM MAP images of the tumor growing in the same nude mouse. The cells initially had ca. 4 × 103 AuNCs per cell. MAP stands for “maximum amplitude projection”.

Eun Chul Cho, et al. Angew Chem Int Ed Engl. ;52(4):1152-1155.
2.
Figure 4

Figure 4. From: A Quantitative Analysis of the Fate of Au Nanocages after Uptake by U87-MG Tumor Cells under Both in vitro and in vivo Conditions.

a) Calibration curve for photoacoustic amplitude (PA) as a function of the number of AuNCs in U87-MG cells. Four sets of cells bearing different numbers of nanocages per cell were injected subcutaneously into the dorsal side of a nude mouse, and the photoacoustic amplitudes were obtained in vivo immediately after injection of the cells. The amplitudes were linearly proportional to the number of nanocages in the cells. b) The change in photoacoustic amplitude of the tumor bearing AuNCs growing in a nude mouse over time (t). c) Total number of AuNCs (Ntotal) estimated from PAM data as a function of tumor growth time (t) by multiplying the number of AuNCs per cell (calculated from the calibration curve) and the number of cells at each time point.

Eun Chul Cho, et al. Angew Chem Int Ed Engl. ;52(4):1152-1155.
3.
Figure 1

Figure 1. From: A Quantitative Analysis of the Fate of Au Nanocages after Uptake by U87-MG Tumor Cells under Both in vitro and in vivo Conditions.

a) A schematic illustration of the major objective of this study: to quantitatively analyze the distributions of AuNCs in U87-MG brain tumor cells during their proliferation in vitro and in vivo. b) A typical TEM image of the AuNCs used for our experiments. c) A schematic of the c(RGDyK)-labeled AuNCs. Abbreviations: PEG, poly(ethylene glycol); X, cyclic RGD that can specifically bind to the integrin receptor on U87-MG cells. d) The number of AuNCs taken up by the U87-MG cells (N) as a function of the concentration of AuNCs (CI) incubated with the cells. The numbers were calculated from UV-vis spectra of the supernatants with reference to a calibration curve.

Eun Chul Cho, et al. Angew Chem Int Ed Engl. ;52(4):1152-1155.
4.
Figure 2

Figure 2. From: A Quantitative Analysis of the Fate of Au Nanocages after Uptake by U87-MG Tumor Cells under Both in vitro and in vivo Conditions.

a, b) Superimposed two-photon and phase contrast images showing (a) a U87-MG mother cell and (b) its two daughter cells after 2 days of culture. The photoluminescence from the cyclic RGD-labeled AuNCs is shown in green. c) A calibration curve for the photoluminescence intensity (IFL) as a function of the number of AuNCs per cell (N) taken up by the cells as obtained from the TPM images. The photoluminescence intensity and N had a linear correlation. d) The N in mother cells and their corresponding daughter cells after division. The data was obtained by averaging the intensities of 14 mother cells and their corresponding daughter cells (see Figure S2), respectively, and the intensities were converted to the number of AuNCs according to the calibration curve. The p value was obtained from analysis of variance (ANOVA).

Eun Chul Cho, et al. Angew Chem Int Ed Engl. ;52(4):1152-1155.

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