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Results: 5

1.
FIGURE 5

FIGURE 5. From: Multifactorial Patterns of Gene Expression in Colonic Epithelial Cells Predict Disease Phenotypes in Experimental Colitis.

Localization of pIgR and IgA in colonic mucosa of mice with experimental colitis: (A) DSS colitis; (B) T cell transfer colitis. Sections of formalin-fixed colons were stained by immunofluorescence for pIgR (green), IgA (red) and nuclei (DAPI; blue). Representative images are shown for each treatment group. Images were captured with a 25X objective.

Aubrey L. Frantz, et al. Inflamm Bowel Dis. 2012 November;18(11):2138-2148.
2.
FIGURE 4

FIGURE 4. From: Multifactorial Patterns of Gene Expression in Colonic Epithelial Cells Predict Disease Phenotypes in Experimental Colitis.

Expression of colonic epithelial biomarkers predicts disease severity in experimental colitis: (A) DSS colitis; (B) T cell transfer colitis. Plots depict linear regression comparing PC1 and PC2 scores for each mouse to body weight at the end of the experiment, colon length (DSS colitis) or the ratio of colon weight to length (T cell transfer colitis), and histological colitis scores. Correlation coefficients (r) and P-values (p) are listed for each regression line.

Aubrey L. Frantz, et al. Inflamm Bowel Dis. 2012 November;18(11):2138-2148.
3.
FIGURE 2

FIGURE 2. From: Multifactorial Patterns of Gene Expression in Colonic Epithelial Cells Predict Disease Phenotypes in Experimental Colitis.

Comparison of biomarker expression in colonic ECs in experimental colitis: (A) DSS colitis; (B) T cell transfer colitis. Colitis was induced as described in Figure 1, and colonic ECs were isolated at the end of the experiments. mRNA levels were measured by qRT-PCR and normalized to β2-microglobulin mRNA. For each model, data from 3 independent experiments were combined and expressed as mean ± SEM (n = 16 for DSS colitis and n = 10 for T cell transfer colitis). Asterisks indicate that the mean for treated mice is different from the mean for control mice (p < 0.05).

Aubrey L. Frantz, et al. Inflamm Bowel Dis. 2012 November;18(11):2138-2148.
4.
FIGURE 3

FIGURE 3. From: Multifactorial Patterns of Gene Expression in Colonic Epithelial Cells Predict Disease Phenotypes in Experimental Colitis.

Multifactorial analysis of biomarker expression patterns in experimental colitis, using data described in Figure 2 for mRNA levels in colonic ECs. (A) Factor analysis was used to reduce data for 5 variables, RelA, A20, pIgR, TNF and MIP-2, into two principal components (PCs). Eigenvalues indicate the proportion of variance among mice in expression of these 5 variables that can be described by PC1 and PC2. Weighted coefficients are listed for each variable comprising PC1 and PC2. (B) PC1 and PC2 scores were calculated for each individual mouse based on the sum of normalized expression levels for each variable multiplied by its corresponding coefficient for PC1 and PC2 (see Materials and Methods). Combined data from 3 independent experiments for each mouse model are expressed as mean ± SEM (n ≤ 14 mice/group for DSS colitis and n = 10 mice/group for T cell transfer colitis). Asterisks indicate that the mean PC values for the indicated comparisons are significantly different (p < 0.05); NS = not significant.

Aubrey L. Frantz, et al. Inflamm Bowel Dis. 2012 November;18(11):2138-2148.
5.
FIGURE 1

FIGURE 1. From: Multifactorial Patterns of Gene Expression in Colonic Epithelial Cells Predict Disease Phenotypes in Experimental Colitis.

Assessment of disease severity in DSS and T cell transfer colitis. (A–C) DSS colitis. Mice were left untreated or administered 2% or 3% DSS in the drinking water for 8 days. (A) Changes in body weight (expressed as a percentage of weight at the beginning of the experiment) and disease activity (see Materials and Methods for an explanation of the disease activity index). Data from 1 of 3 independent experiments with similar results are expressed as mean ± SEM (n = 8). (B) Colon lengths were measured on day 8; data combined from 3 independent experiments are expressed as mean ± SEM (n = 16). (A,B) One asterisk indicates that the mean is significantly different from the mean for control mice, and two asterisks indicate that the mean is significantly different from the mean for both control mice and mice treated with 2% DSS (p < 0.05). (C) Colon histology from representative mice at day 8. Images of formalin-fixed colon tissues stained with hematoxylin and eosin were captured with a 20X objective.
(D–F) T cell transfer colitis. Rag1−/− mice were left untreated (control) or adoptively transferred with 4 × 105 CD4+CD45RBhiCD25 T-effector cells (Teff), with or without 1 × 105 CD4+CD45RBloCD25+ T-regulatory cells (Treg). (D) Changes in body weight; data from 1 of 3 independent experiments with similar results are expressed as mean ± SEM (n = 8). (E) Colons were measured and weighed at 10 weeks post-transfer; data are expressed as mean ± SEM (n = 10). (D, E) An asterisk indicates that the mean is significantly different from the mean for control Rag1−/− mice (p < 0.05). (F) Colon histology from representative mice at 10 weeks post-transfer. Images of formalin-fixed colon tissues stained with hematoxylin and eosin were captured with a 20X objective.

Aubrey L. Frantz, et al. Inflamm Bowel Dis. 2012 November;18(11):2138-2148.

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