Display Settings:

Items per page
We are sorry, but NCBI web applications do not support your browser and may not function properly. More information

Results: 7

1.
Figure 6 

Figure 6 . From: Genetic Variation in Saccharomyces cerevisiae: Circuit Diversification in a Signal Transduction Network.

The Rpi1pS288c protein is hyperphosphorylated. Shown is Western blot analysis of Rpi1p phosphorylation state in strains expressing either 3× flag-tagged RPI1S288c or RPI1Sigma. Samples were treated with either buffer or λ-phosphatase.

Brian L. Chin, et al. Genetics. 2012 December;192(4):1523-1532.
2.
Figure 3 

Figure 3 . From: Genetic Variation in Saccharomyces cerevisiae: Circuit Diversification in a Signal Transduction Network.

RPI1 alleles vary in the number of intragenic repeats. The S288c and Sigma alleles of RPI1 have intragenic repeats, but the repeat lengths differ between the two strains. The schematic illustrates the alignment of the two alleles. The boxes represent individual repeat elements and arrowheads represent locations of SNPs. Open areas represent the shortened repeat length in that allele.

Brian L. Chin, et al. Genetics. 2012 December;192(4):1523-1532.
3.
Figure 5 

Figure 5 . From: Genetic Variation in Saccharomyces cerevisiae: Circuit Diversification in a Signal Transduction Network.

RPI1S288c shows strain-independent localization to the MIT1 and FLO11 promoters. (A and B) Localization of Rpi1p using FLAG-tagged alleles in Sigma and S288c assayed by ChIP followed by qPCR for enrichment at (A) −1.3 kb in the FLO11 promoter and (B) −1 kb in the MIT1 promoter. Data were normalized to ACT1 and are expressed as the mean fold enrichment ± SD. *P < 0.01 compared to untagged.

Brian L. Chin, et al. Genetics. 2012 December;192(4):1523-1532.
4.
Figure 2 

Figure 2 . From: Genetic Variation in Saccharomyces cerevisiae: Circuit Diversification in a Signal Transduction Network.

S288c with FLO11prSigma::FLO11 is fMAPK independent. Agar adhesion assays were performed on S288c strains (right half of the plate) or Sigma strains (left half of the plate) in the FLO11 promoter swap experiment (see text). The same plate is shown before (left) and after (right) washing. Strains with their endogenous FLO11 promoter are labeled with their relevant genotype. Strains carrying a swapped FLO11 promoter are labeled numerically: (1) S288c FLO11prSigma::FLO11; (2) S288c FLO11prSigma::FLO11, tec1Δ; (3) Sigma FLO11prS288c::FLO11, tec1Δ; and (4) Sigma FLO11prS288c::FLO11.

Brian L. Chin, et al. Genetics. 2012 December;192(4):1523-1532.
5.
Figure 7 

Figure 7 . From: Genetic Variation in Saccharomyces cerevisiae: Circuit Diversification in a Signal Transduction Network.

Many S288c genes differ from Sigma genes due to changes in intragenic tandem repeats. Twenty-four of the 107 genes predicted to differ between S288c and Sigma in the length of internal repeats were examined by PCR. Twenty-two of these genes had the predicted size difference. Five genes are shown and the results for the other genes are shown in Figure S7. PGD1 and SPT8 have two repeat regions that both change in size. For each pair the left sample is the S288c product and the right sample is the Sigma product.

Brian L. Chin, et al. Genetics. 2012 December;192(4):1523-1532.
6.
Figure 1 

Figure 1 . From: Genetic Variation in Saccharomyces cerevisiae: Circuit Diversification in a Signal Transduction Network.

The fMAPK pathway is not required for FLO11 expression in S288c. (A) Adhesion assays performed on S288c strains (right half of the plate) or Sigma strains (left half of the plate). The same plate is shown before (top) and after (bottom) washing. (B) Pseudohyphal growth on SLAD media for diploid Sigma, S288c, or Sigma/S288c hybrids. (C) qPCR assay of FLO11 transcript levels was performed on Sigma and S288c strains that were WT or tec1Δ. Mean FLO11 levels normalized to ACT1 levels are presented ± SD. *P < 0.01 compared to WT.

Brian L. Chin, et al. Genetics. 2012 December;192(4):1523-1532.
7.
Figure 4 

Figure 4 . From: Genetic Variation in Saccharomyces cerevisiae: Circuit Diversification in a Signal Transduction Network.

RPI1S288c can partially bypass the fMAPK pathway for agar adhesion and FLO11 expression. (A) Agar adhesion of S288c and Sigma strains carrying reciprocal allele swaps of RPI1. The top row shows adhesion assays performed on S288c strains grown on YPD and the bottom row shows adhesion assays performed on Sigma strains grown on synthetic media (see Materials and Methods). The same plates are shown before and after washing. (B and C) qPCR assay of FLO11 transcript levels performed on (B) S288c strains grown in synthetic media and (C) Sigma strains grown on YPD. Mean FLO11 levels normalized to ACT1 levels are presented ± SD. **P < 0.01. Strains with their endogenous RPI1 allele are labeled with their relevant genotype. Strains carrying a swapped RPI1 allele are labeled numerically: (1) S288c RPI1Sigma; (2) S288c RPI1Sigma, tec1Δ; (3) Sigma RPI1S288c; and (4) Sigma RPI1S288c, tec1Δ.

Brian L. Chin, et al. Genetics. 2012 December;192(4):1523-1532.

Display Settings:

Items per page

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk