Results: 2

1.
Figure 2 

Figure 2 . From: Sister Chromatids Segregate at Mitosis Without Mother-Daughter Bias in Saccharomyces cerevisiae.

There is no mother–daughter bias in the segregation of Watson and Crick-containing chromatids during mitosis. (A) The labeling protocol is shown with the BrdU-containing strands of DNA in red and the unlabeled DNA in black. The original Watson (W) and Crick (C) strands are indicated, as are centromeres (circles). (B) Normalized mean raw intensities for individual genes on the Watson strand (red) or the Crick strand (blue) of chromosome 5 vs. the position along the chromosome. (C) The log2 ratios of intensities for every gene on the Watson strand (red) and the Crick strand (blue) vs. the position along the chromosome. (D) Q-Q plot of the log2 ratio for the Watson strand of chromosome 5. (E) Plot of the distribution of the intensities of log2 ratios for the Watson strand (red) and the Crick strand (blue) for chromosome 5.

Brice E. Keyes, et al. Genetics. 2012 December;192(4):1553-1557.
2.
Figure 1 

Figure 1 . From: Sister Chromatids Segregate at Mitosis Without Mother-Daughter Bias in Saccharomyces cerevisiae.

Watson and Crick-containing chromatids are not exclusively segregated to mother or daughter cells. (A) The labeling protocol is shown with the BrdU-containing strands of DNA in red and the unlabeled DNA in black. The original Watson (W) and Crick (C) strands are indicated, as are centromeres (circles). Cells are labeled with BrdU in the first cell cycle, and mothers and daughters are separated and grown for a second cell cycle in the presence of unlabeled thymidine (TdR). Mothers derived from the first mother (MM) are purified from the daughters (MD) and similarly for the mothers derived from the first daughter (DM) and the corresponding daughter (DD) after the second cell cycle. (B) Short and long exposures of the Southwestern blot to detect BrdU. All experiments were performed in strain CVY63 MATa ade2-1 trp1-1 can1-100 leu2-3,112, his3-11,15 bar1::hisG LEU2:BrdU-inc, which is isogenic with W303a and was kindly supplied by Oscar Aparicio. All methods are in File S1.

Brice E. Keyes, et al. Genetics. 2012 December;192(4):1553-1557.

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