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1.
Figure 6

Figure 6. From: Fas-deficient mice have impaired alveolar neutrophil recruitment and decreased expression of anti-KC autoantibody:KC complexes in a model of acute lung injury.

Neutrophil chemotaxis. Chemotaxis towards KC of peripheral blood neutrophils isolated from lpr and B6 mice. Note the increased response of neutrophils from lpr mice at the higher doses of KC. * = p < 0.05, n = 3.

Sucheol Gil, et al. Respir Res. 2012;13(1):91-91.
2.
Figure 9

Figure 9. From: Fas-deficient mice have impaired alveolar neutrophil recruitment and decreased expression of anti-KC autoantibody:KC complexes in a model of acute lung injury.

The detection of anti-KC:KC immune complex is specific. Mouse PMN were purified and incubated with either anti-KC-KC immune complexes (first two columns) or with KC alone (third column). Note that the Peprotech antibody, detected only anti-KC-KC immune complexes, whereas the R&D antibody detected only KC. The FcγRIII was labeled green and KC was labeled red.

Sucheol Gil, et al. Respir Res. 2012;13(1):91-91.
3.
Figure 10

Figure 10. From: Fas-deficient mice have impaired alveolar neutrophil recruitment and decreased expression of anti-KC autoantibody:KC complexes in a model of acute lung injury.

Expression of FcγRIII is lower in lpr mice. C57BL/6 (B6) and Fas-deficient lpr mice received intratracheal installations of E. coli LPS, 15 ng/kg, followed by 4 hrs of mechanical ventilation (MV) with tidal volumes of 10 mL per kilogram, FiO2 of 0.21, PEEP = 3 and respiratory rate = 150 breaths per minute. Note decreased expression of FcγRIII (green) in the lpr mice. Nuclei are labeled blue.

Sucheol Gil, et al. Respir Res. 2012;13(1):91-91.
4.
Figure 7

Figure 7. From: Fas-deficient mice have impaired alveolar neutrophil recruitment and decreased expression of anti-KC autoantibody:KC complexes in a model of acute lung injury.

Soluble KC:antiKC immune complexes in BAL fluid. Soluble anti-KC:KC immune complexes as measured by ELISA in the BAL fluid from B6 and lpr mice allowed to breath spontaneously, or treated with E. coli LPS followed by 4 hours of mechanical ventilation with Tv = 10 cc/kg, FiO2 = 0.21, PEEP = 3. Note that the B6 mice had a significant fold increase in immune complex concentrations as compared with the lpr mice.

Sucheol Gil, et al. Respir Res. 2012;13(1):91-91.
5.
Figure 8

Figure 8. From: Fas-deficient mice have impaired alveolar neutrophil recruitment and decreased expression of anti-KC autoantibody:KC complexes in a model of acute lung injury.

Anti-KC autoantibody:KC complex deposition in the lungs. FcγRIII (green) and KC (red) in tissue sections from B6 (A) and lpr (B) mice treated with intratracheal E. coli LPS, 15 ng/kg, and exposed to 4 hours of mechanical ventilation (MV) at Tv = 10 cc/kg, FiO2 = 0.21, PEEP = 3. FcγRIII is shown in green, KC is shown in red, and Gr1 (staining leukocytes) is shown in pink. Nuclei are shown in blue. There is an increase signal for both KC and FcγRIII in the B6 mice as compared with the lpr mice. Note the colocalization of KC and FcγRIII.

Sucheol Gil, et al. Respir Res. 2012;13(1):91-91.
6.
Figure 2

Figure 2. From: Fas-deficient mice have impaired alveolar neutrophil recruitment and decreased expression of anti-KC autoantibody:KC complexes in a model of acute lung injury.

Permeability response. Total BAL protein (A) and concentrations of the large molecular weight protein alpha macroglobulin (B) in the BAL fluid of C57BL/6 (B6) or Fas-deficient lpr mice that received intratracheal installations of either PBS or LPS, followed by either spontaneous breathing (SB) or four hours of mechanical ventilation (MV). There were no significant differences among any of the groups studied. n = at least 6/group.

Sucheol Gil, et al. Respir Res. 2012;13(1):91-91.
7.
Figure 5

Figure 5. From: Fas-deficient mice have impaired alveolar neutrophil recruitment and decreased expression of anti-KC autoantibody:KC complexes in a model of acute lung injury.

Distal Organ Injury. Creatinine (A), alanine aminotransferase (ALT) (B) and total bilirubin (C) in the serum of C57BL/6 (B6) or Fas-deficient lpr mice exposed to either intratracheal instillations of PBS followed by spontaneous breathing (SB), or intratracheal instillations of LPS, 15 ng/kg, followed by 4 hr of mechanical ventilation (MV) with Tv = 10 cc/kg, FiO2 = 0.21% and PEEP = 3. At the times tested, the combination of MV + LPS resulted in an increase in creatinine, but not ALT or bilirrubin. There was no difference in the creatinine concentrations of the B6 or lpr mice. n = at least 6/group.

Sucheol Gil, et al. Respir Res. 2012;13(1):91-91.
8.
Figure 4

Figure 4. From: Fas-deficient mice have impaired alveolar neutrophil recruitment and decreased expression of anti-KC autoantibody:KC complexes in a model of acute lung injury.

Tissue respons. C57BL/6 or Fas-deficient lpr mice were exposed to either intratracheal installations of PBS followed by spontaneous breathing or to intratracheal installation of LPS, 15 ng/kg, followed by four hours of mechanical ventilation (MV) with tidal volumes of 10 mL per kilogram. Lung tissue sections were stained with hematoxylin and eosin to demonstrate the pattern of injury. The instillate contained 2.5% colloidal carbon to identify the instilled areas. Spontaneously breathing mice instilled with PBS showed normal lung architecture, regardless of strain (A, C). The B6 mice exposed to MV and LPS showed intra-alveolar and interstitial neutrophilic infiltrates, as well as thickening of the alveolar walls and occasional fibrin deposition (B).

Sucheol Gil, et al. Respir Res. 2012;13(1):91-91.
9.
Figure 3

Figure 3. From: Fas-deficient mice have impaired alveolar neutrophil recruitment and decreased expression of anti-KC autoantibody:KC complexes in a model of acute lung injury.

Apoptotic response. Caspase-3 activity (A) and Poly ADP ribose polymerase (PARP) (B) in the lungs of C57BL/6 (B6) or Fas-deficient LPR mice treated with intratracheal installations of either PBS or E. coli LPS, 15 ng/kg, followed by either spontaneous breathing (SP) or four hours of mechanical ventilation (MV) with tidal volumes of 10 mL per kilogram. Caspase-3 activity was significantly higher in the lpr mice exposed to MV + LPS. n = at least 6/group. Double-labeling for TUNEL (green) and cytokeratin (red) reveals that the TUNEL positive cells are located in the alveolar wall, but most of them are cytokeratin negative.

Sucheol Gil, et al. Respir Res. 2012;13(1):91-91.
10.
Figure 1

Figure 1. From: Fas-deficient mice have impaired alveolar neutrophil recruitment and decreased expression of anti-KC autoantibody:KC complexes in a model of acute lung injury.

Inflammatory response. C57BL/6 (B6) and Fas-deficient lpr mice received intratracheal installations of either PBS followed by four hours of spontaneous breathing (SB), or E. coli LPS, 15 ng/kg, followed by 4 hrs of mechanical ventilation (MV) with tidal volumes of 10 mL per kilogram, FiO2 of 0.21, PEEP = 3 and respiratory rate = 150 breaths per minute. In response to the combination of MV and LPS, the B6 mice showed significantly more total neutrophils (PMN) in the BAL fluid than the lpr mice (A). A similar pattern was seen for the lung MPO activity, which is a measure of the total neutrophil content in the lung (B). MV + LPS was associated with increases in the lung homogenate concentrations of the cytokinesTNF-α, MIP-2, and KC, and these similar in the B6 and the lpr mice; MV + LPS had no effect on IL-1β (C-F). n = at least 6/group.

Sucheol Gil, et al. Respir Res. 2012;13(1):91-91.

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