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Results: 4

1.
Fig. 2

Fig. 2. From: Circadian genes Period 1 and Period 2 in the nucleus accumbens regulate anxiety-related behavior.

Mice with mutations in the Per genes have a normal locomotor response to novelty. Locomotor activity of (A) mPer1 (n = 5), (B) mPer2 (n = 14), and (C) mPer1;mPer2 (n = 9) mutant mice was not significantly different from WT littermates (n = 20) by two-way ANOVA.

Sade Spencer, et al. Eur J Neurosci. ;37(2):242-250.
2.
Fig. 3

Fig. 3. From: Circadian genes Period 1 and Period 2 in the nucleus accumbens regulate anxiety-related behavior.

Chronic stress leads to a decrease in mPer1 and mPer2 expression in the NAc. WT mice were subjected to 10 days of social defeat stress followed by 28 days of vehicle (veh) (n = 8) or fluoxetine (n = 6) treatment (pellets implanted s.c.). Control mice were only subjected to the 28 days of vehicle (n = 7) or fluoxetine pellets (n = 6), but no stress. mPer1 and mPer2 mRNA expression was significantly decreased in the NAc following chronic stress (*p<0.05) (A and B). Fluoxetine treatment following social defeat stress resulted in expression levels that were not different from control animals.

Sade Spencer, et al. Eur J Neurosci. ;37(2):242-250.
3.
Fig. 1

Fig. 1. From: Circadian genes Period 1 and Period 2 in the nucleus accumbens regulate anxiety-related behavior.

mPer1;mPer2 mutant mice display increased anxiety-related behavior. (A) mPer1;mPer2 mutant mice spent less time in the lighted compartment of the dark/light box. *P < 0.05, one-way ANOVA followed by Bonferroni post-hoc test (n = 25, WT; n = 7, mPer1; n = 12, mPer2; n = 11, mPer1/2). (B) mPer1;mPer2 mutant mice had less locomotor activity in the light as measured by beam breaks in the lit compartment of a dark/light box compared with WT mice. *P < 0.05, one-way ANOVA followed by Bonferroni post-hoc test (n = 20, WT; n = 8, mPer1; n = 14, mPer2; n = 9, mPer1/2). (C) mPer2 and mPer1;mPer2 mutant mice spent less time in the open arms of the EPM. *P < 0.05, one-way ANOVA followed by Bonferroni post-hoc test (n = 25, WT; n = 7, mPer1; n = 12, mPer2; n = 11, mPer1/2). (D) Open arm entry frequency in the EPM (n = 20, WT; n = 8, mPer1; n = 14, mPer2; n = 9, mPer1/2).

Sade Spencer, et al. Eur J Neurosci. ;37(2):242-250.
4.
Fig. 4

Fig. 4. From: Circadian genes Period 1 and Period 2 in the nucleus accumbens regulate anxiety-related behavior.

Knockdown of mPer1 and mPer2 in the NAc increases anxiety. Mice were infused with the AAV-mPer1;mPer2 shRNA (sh) or AAV-scrambled control shRNA (Scr) into the NAc and behavior was measured 2 weeks later. (A) Representative image showing viral expression in the NAc and DAPI staining of the same slice. Scale bar, 500 µm. (B) AAV-mPer1;mPer2 infusion leads to a significant knockdown of mPer1 and mPer2 expression in the NAc (n = 6, Scr-infected mice; n = 5–6 mPer1;mPer2 shRNA-infected mice with two biological replicates of each). (C)AAV-mPer1;mPer2 shRNA NAc-infected mice (n = 10) had fewer open arm entries in the EPM than controls (n = 11) (P < 0.05, t-test). (D) AAV-mPer1;mPer2 shRNA NAc-infected mice spent less time in the center of the open field (*P<0.05, t-test).

Sade Spencer, et al. Eur J Neurosci. ;37(2):242-250.

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