Results: 5

1.
Figure 1

Figure 1. Intracellular pathogens or IL-12 restore lymphoid CD8α+ cDCs and tissue-resident CD103+ cDCs in Batf3−/− mice. From: Compensatory dendritic cell development mediated by BATF-IRF interactions.

a, Wild type (WT) and Batf3−/− (BATF3 KO) 129SvEv mice were uninfected (CTL) or infected with Mtb, and spleens harvested and analyzed by FACS at the indicated time. Histograms for indicated markers are gated as autofluorescentMHCIIhighCD11c+ cells. Numbers are percent of cells in the gate. b, Serum IL-12 was measured from individual mice (a) at the indicated time. c, Wild type (WT) and Batf3−/− (BATF3 KO) 129SvEv mice were treated with vehicle (PBS) or IL-12 (IL12) and analyzed by FACS after 3 days as in (a).

Roxane Tussiwand, et al. Nature. 2012 October 25;490(7421):502-507.
2.
Figure 3

Figure 3. Batf compensates for CD8α+ cDC development in Batf3−/− mice. From: Compensatory dendritic cell development mediated by BATF-IRF interactions.

a, Wild type (WT), or Batf3−/− (BATF3KO) BM cells were infected with GFP-RV33 (Empty) or retrovirus expressing the indicated cDNA and cultured with Flt3L7. Histograms for the indicated markers are for B220CD11c+ cells on day 10. Numbers are the percent of cells in the gate. b, Inguinal lymph nodes from WT, Batf3−/− (BATF3 KO) or Batf−/−Batf3−/− mice (BATF1/3 DKO) on 129SvEv or C57BL/6 backgrounds were analyzed by FACS. Shown are histograms for DEC205 and CD8α. c, CD4 T cells of the indicated genotype were differentiated twice under TH2 conditions5 and analyzed by FACS for intracellular IL-10.

Roxane Tussiwand, et al. Nature. 2012 October 25;490(7421):502-507.
3.
Figure 2

Figure 2. IL-12-induced CD8α+ cDCs in Batf3−/− mice can cross-present and mediate tumor rejection. From: Compensatory dendritic cell development mediated by BATF-IRF interactions.

a, From mice in Fig. 1c, DCs were purified by sorting as CD3DX5MHCII+CD11c+Sirp-αCD24+DEC205+ DCs (CD8DC) and CD3DX5MHCII+CD11c+Sirp-α+CD24DEC205 DCs (CD4DC) and assayed for cross-presentation7. OT-I proliferation in response to cDCs mixed with the indicated number of MHC class I-deficient ovalbumin (Ova)-loaded splenocytes is shown. b, Wild type (WT) or Batf3−/− (BATF3 KO) mice treated with vehicle (PBS) or with IL-12 (IL12) were inoculated with 1×106 H31m1 fibrosarcomas. Tumor size in individual mice is shown. c, Mice in (b) were analyzed by FACS 11 days after H31m1 inoculation for CD8 T cell infiltration into tumors7.

Roxane Tussiwand, et al. Nature. 2012 October 25;490(7421):502-507.
4.
Figure 4

Figure 4. Batf2 compensates for Batf3 in CD8α+ and CD103+ cDC development during T. gondii infection. From: Compensatory dendritic cell development mediated by BATF-IRF interactions.

a, Wild-type (WT) and Batf2−/− (BATF2KO) mice were infected with T. gondii and monitored for survival. n=29 for WT (dashed line) and Batf2−/− (solid line) mice. b, Shown are percentages of lung CD103+ DCs of total CD45.2+ cells for uninfected and infected (T. gondii) mice on day 10. n=5 from one of three experiments. c, WT or Batf3−/− (BATF3KO) BM cells were infected with the indicated retrovirus, cultured with Flt3L and analyzed by FACS on day 10. d, Groups of 5 mice each of the indicated genotypes were treated with vehicle (PBS) or IL-12 (IL12) and analyzed by FACS after 3 days. Shown are percentages of CD8α+ cDCs as a total of splenic cDCs.

Roxane Tussiwand, et al. Nature. 2012 October 25;490(7421):502-507.
5.
Figure 5

Figure 5. BATF leucine zipper interactions with non-AP-1 factors mediate lineage-specific actions. From: Compensatory dendritic cell development mediated by BATF-IRF interactions.

a, Structures of chimeric proteins are shown below a diagram of c-Fos. DNA binding domain (DB), hinge (H), leucine zipper (LZ), amino- (5′) and carboxy-terminus (3′). Flt3L-treated WT or Batf3−/− (BATF3 KO) BM infected with the indicated retrovirus were analyzed after 10 days. b, 293FT cells expressing both Batf and Irf4 (upper panel) or Batf and Irf4 as indicated (lower panel) were analyzed by EMSA with the indicated probes and antibodies c, 293FT cells expressing Irf4 (+) and the indicated Batf chimera were analyzed by EMSA with the AICE1 probe. d, B cells were analyzed by EMSA with the indicated probe and competitor oligonucleotides (comp). e, Batf3−/− (BATF3 KO) BM infected with the indicated Batf retroviruses s encoding Batf were analyzed as in (a). f, 293FT cells expressing the indicated Batf mutants were analyzed by EMSA with the AICE1 probe.

Roxane Tussiwand, et al. Nature. 2012 October 25;490(7421):502-507.

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