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2.
Figure 6

Figure 6. Expression of miR-151-5p in PCa specimens.. From: Genistein Suppresses Prostate Cancer Growth through Inhibition of Oncogenic MicroRNA-151.

(A) miR-151 expression in clinical samples (BPH, n = 17; PCa, n = 30). **,P<0.0001 (B) Kaplan-Meier survival curve showing overall survival by miR-151a-5p expression (P = 0.0944).

Takeshi Chiyomaru, et al. PLoS One. 2012;7(8):e43812.
3.
Figure 2

Figure 2. Effect of miR-151a-5p knockdown on prostate cancer cell migration and invasion.. From: Genistein Suppresses Prostate Cancer Growth through Inhibition of Oncogenic MicroRNA-151.

(A) Knockdown of miR-151a-5p significantly inhibits cell migration. After transfection (48 hours), a wound was formed by scraping and the wound measured after 24 hours. Representative images of wound healing assay are shown at x200 magnification. **,P<0.0001 (B) Knockdown of miR-151a-5p significantly decreased cell invasion. Representative images of invasion assay are shown at x200 magnification. **,P<0.0001.

Takeshi Chiyomaru, et al. PLoS One. 2012;7(8):e43812.
4.
Figure 4

Figure 4. Luciferase reporter assays using the vectors encoding putative target sites of 3′UTR.. From: Genistein Suppresses Prostate Cancer Growth through Inhibition of Oncogenic MicroRNA-151.

PC-3 cells were transiently transfected with Pre-miR miRNA precursor or Anti-miR miRNA Inhibitor or negative control, followed by transient transfection with wild-type 3′UTR reporter plasmids or mutated 3′UTR plasmids for 24 hours. 3′UTR reporter activity was measured by luciferase assay and normalized to activity of Renilla luciferase. Data are presented as the mean ± SE. *,P<0.05.

Takeshi Chiyomaru, et al. PLoS One. 2012;7(8):e43812.
5.
Figure 1

Figure 1. Expression of miR-151 in PCa cells and effect of genistein treatment.. From: Genistein Suppresses Prostate Cancer Growth through Inhibition of Oncogenic MicroRNA-151.

(A) Expression profile of miR-151 in PCa cell lines (LNCaP, DU145 and PC3) and normal prostate epithelial cells (RWPE-1). Real-time PCR showed that the expression levels of miR-151 were up-regulated in androgen-independent PCa cell lines (DU145 and PC3). miR-151 expression was normalized to RNU48. Data are presented as means ± SE. (B) Expression levels of miR-151 after treatment with genistein (25 µM). miR-151 expression was found to be reduced by 15–45% in genistein treated cells. *,P<0.05.

Takeshi Chiyomaru, et al. PLoS One. 2012;7(8):e43812.
6.
Figure 5

Figure 5. miR-151a-5p targets CASZ1, IL1RAPL1, SOX17, N4BP1 and ARHGDIA genes.. From: Genistein Suppresses Prostate Cancer Growth through Inhibition of Oncogenic MicroRNA-151.

(A) (B) (C) (D) (E) The mRNA levels of five target genes of miR-151a-5p were determined by quantitative real-time PCR analyses after transfection with miR-151a-5p mimics, miR-151a-5p inhibitor and negative control in PCa cell lines (PC3 and DU145). (F) The mRNA levels of five target genes of miR-151a-5p were determined by quantitative real-time PCR analyses after treatment with genistein (25 µM) in PCa cell lines (DU145). (G) The protein levels of target genes of miR-151a-5p were determined by western blot analyses after transfection with miR-151a-5p mimics, miR-151a-5p inhibitor and negative control in PCa cell lines (PC3 and DU145). Data are presented as the mean ± SE. *,P<0.05.

Takeshi Chiyomaru, et al. PLoS One. 2012;7(8):e43812.

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