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Results: 5

1.
Fig. 1

Fig. 1. From: Integrin ?v?3-Targeted IRDye 800CW Near-Infrared Imaging of Glioblastoma.

Expression of integrin β3 (red) in the RCAS-PDGF (A), U-87 MG (B) and TS543 (C) glioblastomas by immunofluorescence co-staining. CD31 was used as a marker of endothelial cells. HA-tag (A), EGFP (B) and human-specific vimentin (C) were used as tumor cell markers for the RCAS-PDGF, U-87 MG and TS543 glioblastomas, respectively. DAPI staining shows nuclei (blue). White scale bars in Panel B and C, 20 μm. Expression of integrin β3 in the cultured U-87 MG and TS543 tumor cells was confirmed by Western blotting (D). β-actin was used as a loading control.

Ruimin Huang, et al. Clin Cancer Res. ;18(20):5731-5740.
2.
Fig. 3

Fig. 3. From: Integrin ?v?3-Targeted IRDye 800CW Near-Infrared Imaging of Glioblastoma.

A. Non-invasive dynamic NIRF in vivo imaging of the RCAS-PDGF (upper panel), U-87 MG (middle panel) and TS543 (lower panel) glioblastoma-bearing mice. IRDye 800CW-RGD alone, IRDye 800CW-RAD alone (nonspecific peptide control) and IRDye 800CW-RGD plus cRGD peptide (blocking assay) were injected intravenously into glioblastoma-bearing mice or into non-tumor control mice from the same litter. ROIs on the photograph indicated the areas shown in the NIRF images at different time points after injection. B. NIRF-800 nm signal intensity from the ROIs was quantified. Error bars, SEM; *, p<0.01.

Ruimin Huang, et al. Clin Cancer Res. ;18(20):5731-5740.
3.
Fig. 5

Fig. 5. From: Integrin ?v?3-Targeted IRDye 800CW Near-Infrared Imaging of Glioblastoma.

Fluorescence-guided, step-wise resection of a RCAS-PDGF glioblastoma using IRDye 800CW-RGD peptide NIRF imaging. A representative glioblastoma-bearing brain was collected 48 hours after IRDye 800CW-RGD injection. The tumor (indicated by the black arrow heads) was exposed and a two-step resection was performed to remove the glioblastoma tissue as illustrated in the photographs (A). Corresponding white-light and NIRF-800 overlay images were obtained using a Pearl Impulse Imager (B). The dissected tumor (C) and the remaining resection bed in the brain (D) were imaged by an Odyssey Imager and analyzed by H&E staining, respectively. Of note, the small red box in the bottom figure of Panel B indicates residual fluorescence in the resection bed, which corresponded to a microscopic tumor focus as demonstrated by H&E staining (D, small red box and magnified view).

Ruimin Huang, et al. Clin Cancer Res. ;18(20):5731-5740.
4.
Fig. 4

Fig. 4. From: Integrin ?v?3-Targeted IRDye 800CW Near-Infrared Imaging of Glioblastoma.

NIRF imaging of the RCAS-PDGF (A&D), U-87 MG (B&E) and TS543 (C&F) glioblastomas ex vivo. Mice brains were dissected 48 hours after injection of IRDye 800CW-RGD alone, IRDye 800CW-RAD alone (nonspecific peptide control) and IRDye 800CW-RGD plus cRGD peptide (blocking assay). Whole brains (A-C) and sliced brains (D-F) were imaged by a Pearl Impulse Imager and an Odyssey Imager, respectively. Tumor areas are indicated by the red arrow heads in the photograph. The black-background in the NIRF-800 columns (A-C) and rows (D-F) was converted to a white-background to facilitate visualization of the fluorescence emissions. Fluorescence at 800 nm from the tumor area and the corresponding normal brain area was quantified (right panel in D-F); error bars indicate SEM. H&E staining was used to confirm the presence of a glioma. Blue scale bars in the inserts, 50 μm.

Ruimin Huang, et al. Clin Cancer Res. ;18(20):5731-5740.
5.
Fig. 2

Fig. 2. From: Integrin ?v?3-Targeted IRDye 800CW Near-Infrared Imaging of Glioblastoma.

A. Specific binding to integrin receptors by IRDye 800CW-labeled RGD and RAD peptides (green). Immunofluorescence staining using the integrin β3 antibody is shown in red, and DAPI staining of nuclei in blue. Four panels are shown: IRDye 800CW-RGD peptide alone, unlabeled RGD peptide (cRGD) alone, IRDye 800CW-RGD plus cRGD peptides (IRDye 800CW-RGD+cRGD) in a blocking assay, and IRDye 800CW-RAD peptide (nonspecific peptide control) alone. B.In vivo NIRF imaging of normal nude mice after intravenous injection of IRDye 800CW-RGD probe with excitation/emission filters for 700 nm and 800 nm, respectively, to assess background fluorescence. Note the markedly lower autofluorescence in 800 nm compared to 700 nm. C. Quantification of normalized fluorescence intensity from whole body images at 700 nm and 800 nm (ROIs indicated in panel B). Error bars indicated standard error of the mean (SEM).

Ruimin Huang, et al. Clin Cancer Res. ;18(20):5731-5740.

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