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Figure 3

Figure 3. Mut-AAV-GFP transfection in the hair cells of neonatal mice 14 days postinoculation.. From: Inner Ear Gene Transfection in Neonatal Mice Using Adeno-Associated Viral Vector: A Comparison of Two Approaches.

A and B: Negative transfection in the hair cells of the collagenase 30 mg/mL group. C–E: Transfection in hair cells at different magnitudes in the collagenase 60 mg/mL subgroup, D: Apical turn, E: Basal turn. F–H: Transfection in hair cells viewed at different magnitudes in the RWM-puncture group. G: Apical turn, H: Basal turn. I: Sparse IHC transfection (arrow) seen in an ear contralateral to that receiving virus via RWM puncture. Scale bars = 50 µm. RWM: round window membrane.

Li Xia, et al. PLoS One. 2012;7(8):e43218.
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Figure 1

Figure 1. Views of surgical areas showing the conditions of RWM after different treatments.. From: Inner Ear Gene Transfection in Neonatal Mice Using Adeno-Associated Viral Vector: A Comparison of Two Approaches.

The insert of each image performed RWM in vitro correspondingly. A: control RWM freshly exposed. The RWM was partially covered by a piece of bone, which was routinely removed during surgery. B: Punctured RWM. The punctured point was covered by a small amount of fluid. C: RWM treated with 30 mg/mL collagenase I, revealing intact RWM with slight congestion. D: RWM treated with 60 mg/mL collagenase I. RWM was intact, but apparent congestion was observed in the RWM and nearby soft tissues, and a scar was seen in the middle of RWM in vitro. E: RWM treated with 90 mg/mL collagenase I, resulting in a large perforation of the RWM, indicated by the crosses. Arrow, RWM; arrowhead, stapedial artery; square, wall of the bulla; circle, lower edge of the round window niche; triangle, the perforation observed in RWM. RWM: round window membrane.

Li Xia, et al. PLoS One. 2012;7(8):e43218.
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Figure 5

Figure 5. Cross-sectional view of the basal turn of cochlea showing cochlear transfection. In the trans-RWM subgroup of RWM treatment with 60 mg/mL collagenase.. From: Inner Ear Gene Transfection in Neonatal Mice Using Adeno-Associated Viral Vector: A Comparison of Two Approaches.

A–C: Strong GFP signals were observed in the IHCs, Claudius cells, membrana tectoria, spiral limbus, and spiral ligament; GFP staining was weak in the OHC (A and B). Few GFP-positive type II SGNs were found (arrows in C), and no staining was observed in type I SGNs. RWM-puncture group D–F: the relative transfection across different cell types and locations was similar to the trans-RWM group, but the GFP signal was generally stronger in the RWM-puncture group. GFP-positive type II SGNs (arrows in F). Arrowhead: type I SGNs. ScV: scala vestibule; ScT: scala tympani; ScM: scala media; OHC: outer hair cell; IHC: inner hair cell; SGNs: spiral ganglion neurons; CC: Claudius cell; DC: Deiters cell; SL: spiral ligament; SV: stria vascularis; MT: membrana tectoria; RM: Reissner's membrane; SPL: spiral limbus; NF: nerve fibers. Scale bars = 50 µm.

Li Xia, et al. PLoS One. 2012;7(8):e43218.

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