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1.
Fig. 4.

Fig. 4. From: The Thyroid Axis Is Regulated by NCoR1 via Its Actions in the Pituitary.

Circulating TH and TSH levels and pituitary expression of TSH subunit mRNA in P-ΔID mice. A, TT4 and total T3 (TT3) levels in control and P-ΔID female (n = 9–11) and male (n = 7–8) mice. B, Plasma TSH levels in female control and P-ΔID mice (n = 9–11). C, Expression of TSH subunit mRNA was measured in the pituitaries of mice of indicated sex and genotype by Q-PCR. The mRNA levels are calculated relative to the control group. Data are presented as mean ± sem; *, P < 0.05 as determined by unpaired t test.

Ricardo H. Costa-e-Sousa, et al. Endocrinology. 2012 October;153(10):5049-5057.
2.
Fig. 3.

Fig. 3. From: The Thyroid Axis Is Regulated by NCoR1 via Its Actions in the Pituitary.

Assessment of localization and efficiency of Cga-Cre-mediated recombination in the pituitary. A, Cga-Cre mice were crossed to a reporter GFP strain that expresses GFP protein in all cells in which Cre activity has been present. Horizontal pituitary sections from resulting Cga-GFP animals were stained with anti-GPF and anti-TSHβ antibodies. Obtained images were merged to assess the colocalization of the Cre-recombinase and TSHβ expression. Original magnification ×40. B, Whole-cell lysates were isolated from pituitaries of female and male control and P-ΔID mice. Protein (20 μg) isolated from two pooled pituitaries were subjected to Western analysis using anti-NCoR1 antibody.

Ricardo H. Costa-e-Sousa, et al. Endocrinology. 2012 October;153(10):5049-5057.
3.
Fig. 6.

Fig. 6. From: The Thyroid Axis Is Regulated by NCoR1 via Its Actions in the Pituitary.

Assessment of possible indirect mechanisms of the regulation of TSH levels in the P-ΔID animals. A, Trhr mRNA levels in euthyroid and hypothyroid (21 d and 63 d of low-iodine/PTU diet) control and P-ΔID mice. B, Animals with indicated genotypes were given 10 μg/kg of recombinant TRH ip. Plasma TSH levels were measured before and 15 and 60 min after the injection (n = 9). C, Dio2 and Dio3 mRNA expression in euthyroid and hypothyroid control and P-ΔID mice. All mRNA expression levels (A, C, and D) were quantified by Q-PCR and are presented relative to euthyroid control group (set to 1). All values are presented as mean ± sem; **, P < 0.01; ***, P < 0.001 as determined by two-way repeated measures ANOVA followed by Bonferroni posttest.

Ricardo H. Costa-e-Sousa, et al. Endocrinology. 2012 October;153(10):5049-5057.
4.
Fig. 1.

Fig. 1. From: The Thyroid Axis Is Regulated by NCoR1 via Its Actions in the Pituitary.

Efficiency of tamoxifen-induced UBC-Cre-mediated recombination in the pituitary and liver of UBC-ΔID female mice. A, NCoR1 mRNA levels were measured to assess the recombination efficiency in the pituitary and liver of UBC-ΔID mice 36 d after tamoxifen treatment using Q-PCR to estimate relative amounts of NCoR1 and NCoRΔID. Shown are data for all animals in the group including those that were not included in the final analysis (with expression levels of NCoRΔID < 50%). Note that NCoRΔID expression is estimated by subtracting WT from total NCoR expression (n = 6–8 animals per group). B, Total protein lysates were isolated from livers of female mice with indicated genotypes 36 d after tamoxifen treatment. The UBC-NCoRlox/lox mice are representative of those with different amounts of recombination. Protein (30 μg) was loaded per lane and subjected to Western analysis using anti-NCoR1 antibody and anti-RNA Polymerase II antibody (loading control). ***, P < 0.001 as determined by unpaired t test.

Ricardo H. Costa-e-Sousa, et al. Endocrinology. 2012 October;153(10):5049-5057.
5.
Fig. 5.

Fig. 5. From: The Thyroid Axis Is Regulated by NCoR1 via Its Actions in the Pituitary.

Plasma TSH levels and pituitary expression of TSH subunit mRNA in P-ΔID mice during induction of hypothyroidism and T3 replacement. A, Female control and P-ΔID mice were put on low iodine/PTU diet to induce hypothyroidism. Circulating TSH was measured at indicated time points after the start of the diet (n = 6). B and C, Pituitary expression of αGSU (B) and TSHβ (C) mRNA after 21 d and 63 d of low-iodine/PTU diet in female control and P-ΔID animals was measured by Q-PCR. The mRNA levels are calculated relative to the control group at baseline (chow diet). D, Plasma TSH concentration was measured in the indicated groups of female mice after 21 d of low-iodine/PTU diet, and 2- to 4-d periods of treatment with increasing concentrations of T3 as shown (n = 7–8). All values are presented as mean ± sem; *, P < 0.05; **, P < 0.01; ***, P < 0.001 as determined by two-way repeated measures ANOVA (A and D) or unpaired t test (B and C).

Ricardo H. Costa-e-Sousa, et al. Endocrinology. 2012 October;153(10):5049-5057.
6.
Fig. 2.

Fig. 2. From: The Thyroid Axis Is Regulated by NCoR1 via Its Actions in the Pituitary.

Circulating TH levels and pituitary mRNA expression in UBC-ΔID mice after tamoxifen treatment. A, Serum TT4 levels were measured in control and UBC-ΔID at the indicated time points after administration of tamoxifen. B, TT4 levels positively correlate with the expression of WT NCoR1 mRNA 36 d after tamoxifen treatment. C–E, Concentrations of circulating free T4 (FT4) (panel C) and total T3 (D), as well as mRNA expression levels of TSH subunits, Trhr and Dio2 (E) in the pituitary of control and UBC-ΔID mice were measured on d 36 after tamoxifen treatment. mRNA levels were quantified by Q-PCR and calculated relative to the control group. F, Expression of known TH targets Thrsp, Dio1, and Fasn in the liver of UBC-ΔID mice 36 d after tamoxifen treatment. mRNA expression levels were quantified by Q-PCR and presented relative to control group. All data presented are from the same cohort of mice (n = 6 animals per group). Data are presented as mean ± sem; **, P < 0.01; ***, P < 0.001 as determined by two-way repeated measures ANOVA (panel A) or unpaired t test (panels C–F).

Ricardo H. Costa-e-Sousa, et al. Endocrinology. 2012 October;153(10):5049-5057.

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