Results: 4

1.
Figure 1

Figure 1. From: Low-level HIV infection of hepatocytes.

Detection of integrated HIV DNA in hepatocytes. Huh7.5 (A), Huh7.5JFH1(B), or Jurkat (D) cells were incubated in the presence of 0, 1, 10, 100, or 1000 uM of the integrase inhibitor raltegravir for one hour prior to and during infection with DNase-treated HIVNL4-3. Huh7.5 (C) cells were incubated in the presence of raltegravir for one hour prior to and during infection with DNase-treated HIVYK-JRCSF. After 24 hours, integrated HIV DNA was quantified in cell lysates by nested real-time PCR. Error bars represent the standard deviation between duplicates.

Ling Kong, et al. Virol J. 2012;9:157-157.
2.
Figure 3

Figure 3. From: Low-level HIV infection of hepatocytes.

Infectious HIV production in hepatocytes. TZM-bl indicator cells were incubated with culture supernatants collected at day 3 post-infection from Huh7.5 or Huh7.5JHF1 cells (A) or Jurkat cells (B) infected with HIVNL4-3 or 100 ng AT-treated HIVNL4-3and tested for β-galactosidase activity. The mean number of positive TZM-bl cells per well is shown with the error bars representing the standard deviation between duplicates. β-gal expression was also evaluated in the presence of 100 uM AZT for one hour prior to and during infection.

Ling Kong, et al. Virol J. 2012;9:157-157.
3.
Figure 4

Figure 4. From: Low-level HIV infection of hepatocytes.

HIV infection of primary hepatocytes. (A) Primary human hepatocytes were incubated with DNase-treated HIVNL4-3 for two hours. After 24 hours, integrated HIV DNA was quantified in cell lysates by nested real-time PCR. (B)TZM-bl indicator cells were incubated with culture supernatants collected at day 3 post-infection from primary human hepatocytes infected with HIVNL4-3 or 100 ng AT-2 treated HIVNL4-3 and tested for β-galactosidase activity. The mean number of positive TZM-bl cells per well is shown with the error bars representing the standard deviation between duplicates. β-gal expression was also evaluated in the presence of 100 uM AZT for one hour prior to and during infection.

Ling Kong, et al. Virol J. 2012;9:157-157.
4.
Figure 2

Figure 2. From: Low-level HIV infection of hepatocytes.

HIV protein expression in hepatocytes. (A) HIV p24 protein levels were quantified by ELISA in culture supernatants from Huh7.5 cells infected with HIVNL4-3 at days 1, 3, 5, and 7 post-infection. Infection was also performed in the presence of 100 uM AZT for one hour prior to and during infection. Error bars represent the standard deviation between duplicates. (B) Huh7.5JFH1 cells were incubated with HIVNL4-3. At days 1, 3, 5, and 7 post-infection, cells were harvested, lysed, and subjected to Western Blot analysis using a rabbit monoclonal Ab (Epitomics) as the primary Ab and a rabbit polyclonal Ab to mouse IgG from (Abcam) as the secondary Ab. As a loading control, GAPDH was detected using a rabbit polyclonal Ab (Santa Cruz Biotechnology).

Ling Kong, et al. Virol J. 2012;9:157-157.

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