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Results: 4

1.
Fig 2

Fig 2. From: Interlaboratory Standardization of the Sandwich Enzyme-Linked Immunosorbent Assay Designed for MATS, a Rapid, Reproducible Method for Estimating the Strain Coverage of Investigational Vaccines.

Comparison RPs at two temperatures for bacterial lysate preparation. For the five laboratories (A to E) that assayed the strains at both temperatures, the predicted RPs were derived for each laboratory, strain, antigen, and temperature combination (n = 75 pairs). The line of identity represents perfect agreement (intercept = 0, slope = 1).

Brian D. Plikaytis, et al. Clin Vaccine Immunol. 2012 October;19(10):1609-1617.
2.
Fig 3

Fig 3. From: Interlaboratory Standardization of the Sandwich Enzyme-Linked Immunosorbent Assay Designed for MATS, a Rapid, Reproducible Method for Estimating the Strain Coverage of Investigational Vaccines.

Scatter plots of pairwise comparisons of RP values derived from ANOVA models, adjusting for laboratory (A to G), antigen, and lysate preparation temperature, from the replicate RPs submitted for analysis. The data are combined over antigens and plotted on a log10 scale. Predicted RPs were derived from ANOVA random-effects models. The number of strains in common between any two laboratories is listed in Table 2. The solid line indicates perfect agreement (intercept = 0, slope = 1).

Brian D. Plikaytis, et al. Clin Vaccine Immunol. 2012 October;19(10):1609-1617.
3.
Fig 1

Fig 1. From: Interlaboratory Standardization of the Sandwich Enzyme-Linked Immunosorbent Assay Designed for MATS, a Rapid, Reproducible Method for Estimating the Strain Coverage of Investigational Vaccines.

Reference MATS strains and robustness of positive bactericidal thresholds (PBTs) for fHbp (A), NHBA (B), and NadA (C). Box plots show the median and interquartile ranges of RPs collected during the present study, combined over laboratories A to G. Vertical lines extend to the most extreme observation that is <1.5 × the interquartile distance (75th to 25th percentiles). Each box represents all replicate assay data for each strain and antigen combined over all laboratories. Dashed and solid horizontal lines indicate PBT and 95% CIs derived according to equation 1.

Brian D. Plikaytis, et al. Clin Vaccine Immunol. 2012 October;19(10):1609-1617.
4.
Fig 4

Fig 4. From: Interlaboratory Standardization of the Sandwich Enzyme-Linked Immunosorbent Assay Designed for MATS, a Rapid, Reproducible Method for Estimating the Strain Coverage of Investigational Vaccines.

(A) Box plots by antigen and laboratory (A to G) for the fold difference between the consensus and observed RPs. Consensus RPs were estimated for each strain and antigen using a random-effects ANOVA model. The box is defined by the 25th and 75th percentiles of the distribution; the horizontal line in the box represents the median (50th percentile) and the asterisks (*) signify the means. Vertical lines extend to the most extreme observation that is <1.5 × the interquartile distance (75th to 25th percentiles). Diamonds and small boxes (♢, □) correspond to moderate and severe outlying assay values, respectively. Each box represents the distribution of all replicate data for all strains within a laboratory and antigen. (B) Plots of within-laboratory CVs by antigen derived using random-effects ANOVA models. (C) Plots of within-strain CVs by antigen using random-effects ANOVA models. For fHbp, NadA, and NHBA, the strains are plotted on the x axis using consensus RPs derived from ANOVA models.

Brian D. Plikaytis, et al. Clin Vaccine Immunol. 2012 October;19(10):1609-1617.

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