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1.
FIG. 4.

FIG. 4. From: Selective Targeting of the Repressive Transcription Factors YY1 and cMyc to Disrupt Quiescent Human Immunodeficiency Viruses.

Histone deacetylase (HDAC) occupancy of the HIV-1 promoter is not affected by depletion of cMyc or YY1. (a) HDAC1, (b) HDAC2, and (c) HDAC3 occupancy of the HIV-1 promoter was not significantly altered after depletion of cMyc or YY1 from 2D10 cells, as determined using a ChIP assay. (d) HDAC1, (e) HDAC2, and (f) HDAC3 occupancy of HIV-1 LTR did not change significantly after depletion of cMyc or YY1 from J89 cells as measured by ChIP assay.

Kirston Barton, et al. AIDS Res Hum Retroviruses. 2013 February;29(2):289-298.
2.
FIG. 1.

FIG. 1. From: Selective Targeting of the Repressive Transcription Factors YY1 and cMyc to Disrupt Quiescent Human Immunodeficiency Viruses.

Depletion of cMyc or YY1 does not affect Jurkat cell viability. Lentiviral shRNAs specific to cMyc, YY1, or cMyc and YY1 significantly deplete the protein levels of cMyc (a), YY1 (b), or cMyc and YY1 (c) in 2D10 cells as determined by western blot analysis. (d) Depletion of cMyc, YY1, or cMyc and YY1 does not affect the viability of 2D10 cells 72 h after transduction of short hairpin RNAs (shRNAs).

Kirston Barton, et al. AIDS Res Hum Retroviruses. 2013 February;29(2):289-298.
3.
FIG. 5.

FIG. 5. From: Selective Targeting of the Repressive Transcription Factors YY1 and cMyc to Disrupt Quiescent Human Immunodeficiency Viruses.

Histone 3 and acetylated histone 3 levels on the HIV-1 promoter did not significantly change following depletion of cMyc or YY1 in Jurkat cells. (a, c) Histone 3 occupancy of the HIV-1 promoter in 2D10 (a) and J89 (c) cells did not significantly change following depletion of cMyc or YY1. (b) The levels of histone 3 acetylation significantly increased after depletion of cMyc in 2D10 cells. However, the level of acetylated histone 3 did not change following knockdown of cMyc or YY1 in J89 cells (d) or after depletion of YY1 in 2D10 cells (b). *p-value of less than 0.05.

Kirston Barton, et al. AIDS Res Hum Retroviruses. 2013 February;29(2):289-298.
4.
FIG. 6.

FIG. 6. From: Selective Targeting of the Repressive Transcription Factors YY1 and cMyc to Disrupt Quiescent Human Immunodeficiency Viruses.

Concurrent knockdown of cMyc and YY1 did not significantly affect HDAC1, 2, or 3 recruitment to the HIV-1 promoter or the levels of histone 3. Depletion of cMyc and YY1 from 2D10 cells did not affect the levels of HDAC1 (a), HDAC2 (b), HDAC3 (c), histone 3 (d), or acetylated histone 3 (e) at the HIV-1 LTR. Similarly, no changes in HDAC occupancy or histone 3 occupancy or acetylation was observed at the HIV-1 LTR following depletion of cMyc and YY1 in J89 cells (f–j) as measured by ChIP.

Kirston Barton, et al. AIDS Res Hum Retroviruses. 2013 February;29(2):289-298.
5.
FIG. 3.

FIG. 3. From: Selective Targeting of the Repressive Transcription Factors YY1 and cMyc to Disrupt Quiescent Human Immunodeficiency Viruses.

Depletion of YY1 significantly increases HIV-1 expression in 2D10 cells. (a) GFP mRNA expression from the HIV-1 LTR significantly increased after depletion of YY1 from 2D10 cells. No changes were observed in GFP mRNA expression levels following depletion of cMyc or cMyc and YY1 from 2D10 cells. (b) The percentage of 2D10 cells expressing the GFP protein, as determined by flow cytometry, significantly increased after depletion of YY1, but not after depletion of cMyc or cMyc and YY1. (c) Expression of gag mRNA was not significantly affected by knockdown of cMyc, YY1, or both in J89 cells. (d) The percentage of J89 cells expressing the GFP protein, as determined using flow cytometry, did not significantly increase after knockdown of cMyc, YY1, or cMyc and YY1. *p-value of less than 0.05.

Kirston Barton, et al. AIDS Res Hum Retroviruses. 2013 February;29(2):289-298.
6.
FIG. 2.

FIG. 2. From: Selective Targeting of the Repressive Transcription Factors YY1 and cMyc to Disrupt Quiescent Human Immunodeficiency Viruses.

Depletion of cMyc, YY1, or cMyc and YY1 does not significantly affect expression of HDAC1, HDAC2, or HDAC3 in 2D10 cells. (a) HDAC1, HDAC2, and HDAC3 protein levels remain stable in 2D10 cells after depletion of cMyc, YY1, or cMyc and YY1. (b) HDAC1, (c) HDAC2, or (d) HDAC3 mRNA expression levels are not significantly affected by the depletion of cMyc, YY1, or cMyc and YY1 in 2D10 cells as measured by the reverse transcription quantitative polymerase chain reaction (PCR) assay (RT-qPCR).

Kirston Barton, et al. AIDS Res Hum Retroviruses. 2013 February;29(2):289-298.
7.
FIG. 7.

FIG. 7. From: Selective Targeting of the Repressive Transcription Factors YY1 and cMyc to Disrupt Quiescent Human Immunodeficiency Viruses.

Targeting YY1 and HDAC activity significantly increases expression from the HIV-1 LTR. Depletion of YY1 in conjunction with the addition of 500 nM SAHA for 18 h resulted in a significant increase in the percentage of 2D10 cells expressing GFP as compared to cells that were transduced with the vector control and treated with SAHA as measured using flow cytometry. Depletion of cMyc or cMyc and YY1 in combination with SAHA did not significantly increase the percentage of 2D10 cells expressing GFP. *p-value of less than 0.05.

Kirston Barton, et al. AIDS Res Hum Retroviruses. 2013 February;29(2):289-298.

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