Display Settings:

Items per page
We are sorry, but NCBI web applications do not support your browser and may not function properly. More information

Results: 6

1.
FIGURE 2.

FIGURE 2. From: Structural Basis of Rev1-mediated Assembly of a Quaternary Vertebrate Translesion Polymerase Complex Consisting of Rev1, Heterodimeric Polymerase (Pol) ?, and Pol ?.

Structure of the quaternary Rev1 CTD-Rev3/7-Pol κ RIR complex. Ribbon diagrams of the complex are shown in stereo view and are colored with the Rev1 CTD in green, Rev3 in yellow, Rev7 in purple, and the Pol κ RIR in cyan.

Jessica Wojtaszek, et al. J Biol Chem. 2012 September 28;287(40):33836-33846.
2.
FIGURE 3.

FIGURE 3. From: Structural Basis of Rev1-mediated Assembly of a Quaternary Vertebrate Translesion Polymerase Complex Consisting of Rev1, Heterodimeric Polymerase (Pol) ?, and Pol ?.

The Rev1 CTD-Pol κ RIR interface. A, structure of the Rev1 CTD-Rev3/7-Pol κ RIR complex superimposed with the NMR ensemble of the free Rev1 CTD (PDB ID: 2LSG). Components of the quaternary complex are colored, with the Rev1 CTD in green, Pol κ RIR in cyan, Rev7 in purple, and Rev3 in yellow. The NMR ensemble of the free Rev1 CTD is shown in Cα traces and colored gray. B, hydrophobic interactions between the Rev1 CTD and Pol κ RIR. C, hydrophilic interactions between the Rev1 CTD and Pol κ RIR.

Jessica Wojtaszek, et al. J Biol Chem. 2012 September 28;287(40):33836-33846.
3.
FIGURE 4.

FIGURE 4. From: Structural Basis of Rev1-mediated Assembly of a Quaternary Vertebrate Translesion Polymerase Complex Consisting of Rev1, Heterodimeric Polymerase (Pol) ?, and Pol ?.

The Rev3/7 interface. A, overall structure of the Rev3/7 complex in the quaternary Rev1 CTD-Rev3/7-Pol κ RIR complex. Rev3 and Rev7 are shown in a ribbon diagram, and the Rev1 CTD and Pol κ RIR are shown in Cα traces. B, Rev3-Rev7 binding interface encompassing the N-terminal α1 helix and the β1 strand of Rev3. C, Rev3-Rev7 interface encompassing the C-terminal α2 helix and the loop connecting the β1 strand and the α2 helix of Rev3.

Jessica Wojtaszek, et al. J Biol Chem. 2012 September 28;287(40):33836-33846.
4.
FIGURE 1.

FIGURE 1. From: Structural Basis of Rev1-mediated Assembly of a Quaternary Vertebrate Translesion Polymerase Complex Consisting of Rev1, Heterodimeric Polymerase (Pol) ?, and Pol ?.

Formation of a quaternary complex consisting of the Rev1 CTD, Rev3/7, and Pol κ RIR. A, 1H-15N HSQC spectra of the free Rev1 CTD (black) and the Rev1 CTD in the presence of an equal molar ratio of either the GB1-Pol κ RIR fusion protein (blue), Rev3/7 (red), or both (purple). B, FPLC traces of the Rev1 CTD-Rev3/7-Pol κ RIR complex (purple), the Rev3/7 complex (light orange), and the Rev1 CTD-Pol κ RIR complex (blue) separated using a HiPrep 26/60 Sephacryl S-200 HR column (GE Healthcare). The elution volumes of known protein standards are labeled.

Jessica Wojtaszek, et al. J Biol Chem. 2012 September 28;287(40):33836-33846.
5.
FIGURE 5.

FIGURE 5. From: Structural Basis of Rev1-mediated Assembly of a Quaternary Vertebrate Translesion Polymerase Complex Consisting of Rev1, Heterodimeric Polymerase (Pol) ?, and Pol ?.

The Rev1 CTD-Rev7 interface. A, hydrophobic interactions between the Rev1 CTD and Rev7. Rev7 residues are labeled in yellow, and Rev1 residues are labeled in green. B, opposite view of A, illustrating the central hydrophobic pocket of the Rev1 CTD that accommodates Pro-188 and Leu-186 of Rev7. Rev7 residues are labeled in purple, and Rev1 residues are labeled in yellow. C and D, hydrophilic interactions between the Rev1 CTD and Rev7, with C showing interactions centered at the α2-α3 loop of the Rev1 CTD and D showing interactions centered at the C-terminal tail of the Rev1 CTD. E, sequence alignment of the Rev1 CTD and Rev7 from mouse (Mus musculus; Mm), chicken (Gallus gallus; Gg), and yeast (S. cerevisiae; Sc). Conserved hydrophobic residues are colored yellow. Hydrophobic residues important for the Rev1-Rev7 interaction are boxed in red.

Jessica Wojtaszek, et al. J Biol Chem. 2012 September 28;287(40):33836-33846.
6.
FIGURE 6.

FIGURE 6. From: Structural Basis of Rev1-mediated Assembly of a Quaternary Vertebrate Translesion Polymerase Complex Consisting of Rev1, Heterodimeric Polymerase (Pol) ?, and Pol ?.

Rev1 is critical for DNA damage tolerance in vertebrate cells. A, wild-type chicken DT40 cells (DT40) and Rev1 deletion chicken DT40 cells (Rev1) harboring full-length wild-type Rev1 (Rev1+WT) or the K1199E mutation (Rev1+K1199E) were treated with the indicated doses of cisplatin (cis-diammineplatinum(II) dichloride), and cell viability was measured 72 h later. B, immunoblot analysis of lysates prepared from Rev1 deletion chicken DT40 cells (−), cells expressing full-length GFP-tagged wild-type Rev1 (Wild-type), and the K1199E mutation (K1199E) probed with an anti-GFP antibody. The same blot was probed with an anti-β-actin antibody as a loading control. Error bars represent standard deviation from three independent experiments.

Jessica Wojtaszek, et al. J Biol Chem. 2012 September 28;287(40):33836-33846.

Display Settings:

Items per page

Supplemental Content

Recent activity

Your browsing activity is temporarily unavailable.

Write to the Help Desk