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1.
Figure 1

Figure 1. From: A Multilocus Sequence Typing System (MLST) reveals a high level of diversity and a genetic component to Entamoeba histolytica virulence.

Amplicon sequencing efficiency for individual samples.A) Number of reads obtained from the Illumina libraries prepared from different sample source x-axis libraries prepared from different sample source; y-axis number of reads (log2 scale) B) Average coverage of the reads when mapped to the concatenated amplicon reference; x-axis libraries prepared from different sample source y-axis average coverage of mapped reads (log2 scale) Line indicates median number of reads.

Carol A Gilchrist, et al. BMC Microbiol. 2012;12:151-151.
2.
Figure 4

Figure 4. From: A Multilocus Sequence Typing System (MLST) reveals a high level of diversity and a genetic component to Entamoeba histolytica virulence.

Amebic culture effect on the EHI_065250 Entamoeba genotype. Distribution of the EHI_065250 SNP at the 10296 location in field isolates or cultured strains established from asymptomatic disease (p = 0.0166). The distribution of the individual SNPs, which were either Reference (Ref), Non-Reference (Non-Ref) or heterologous was shown on the x-axis. The number of samples of with this genotype isolated from patients with asymptomatic disease was shown on the y-axis.

Carol A Gilchrist, et al. BMC Microbiol. 2012;12:151-151.
3.
Figure 2

Figure 2. From: A Multilocus Sequence Typing System (MLST) reveals a high level of diversity and a genetic component to Entamoeba histolytica virulence.

Similarity of E. histolytica diversity in Bangladeshi and whole genome sequenced strains. Shown on the y axis (H) is the calculated heterozygosity and represents sum of the squared allele frequencies was subtracted from 1 on the x axis the loci containing the SNPs genotyped by MSLT(■ value in Bangladesh samples genotyped during this study, (□ value in the sequenced genomes described in Table 1).

Carol A Gilchrist, et al. BMC Microbiol. 2012;12:151-151.
4.
Figure 5

Figure 5. From: A Multilocus Sequence Typing System (MLST) reveals a high level of diversity and a genetic component to Entamoeba histolytica virulence.

SNPs 1&2 in the EHI_080100 locus segregate with disease. Distribution of the SNP1 which was either Reference (□,MS)(Ref), Non-Reference (■ ALA);(Non-Ref) was shown on the x-axis. The number of samples of with this genotype isolated from patients with either amebic liver abscesses diarrhea/(D/D) asymptomatic disease COL was shown on the y-axis. Fisher’s pairwise comparison between asymptomatic and diarrhea/dysentery p = 0.0182 (*); between amebic liver abscess and diarrhea/dysentery samples p = 0.0003; q = 0.0144 (**); Chi-squared contingency analysis of all phenotypes p = 0.002; q = 0.032 (**).

Carol A Gilchrist, et al. BMC Microbiol. 2012;12:151-151.
5.
Figure 6

Figure 6. From: A Multilocus Sequence Typing System (MLST) reveals a high level of diversity and a genetic component to Entamoeba histolytica virulence.

The locationof the SNPs1&2 in EHI_080100 and EHI_065250 genes. Mapping of the informative SNPs within the coding sequences. A) EHI_065250 and B) EHI_080100 genes. Nucleotide position of the amplicon 5’ and 3’ bases are shown and approximate location of the 5’ (green) and 3’ (red) and the positions and number of the targeted SNPs indicated by vertical lines. The bases involved are bracketed in the nucleotide sequence at this region (shown above). The amino acid sequence with changed residues in red is also shown.

Carol A Gilchrist, et al. BMC Microbiol. 2012;12:151-151.
6.
Figure 3

Figure 3. From: A Multilocus Sequence Typing System (MLST) reveals a high level of diversity and a genetic component to Entamoeba histolytica virulence.

Lack of consistent patterns of descent among SNP markers from Bangladeshi E. histolyticaisolates suggests they segregate independently. Consensus phylogeny inferred from 100 bootstrap replicates of polymorphic SNP markers, constructed using the MEGA 5 program and the Maximum Likelihood method based on the Tamura-Nei model and using the sequences shown in Additional file 1: Table 8 [42]. Branches produced in fewer than 50% of the bootstrap phylogenies were collapsed. Sequences from stool have the suffix s; culture c; monthly survey stools begin with MS or CMS, diarrheal DS or CDS, amebic liver abscess samples RUF.

Carol A Gilchrist, et al. BMC Microbiol. 2012;12:151-151.

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