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Results: 6

1.
Fig. 1

Fig. 1. From: Sex Specific Expression of Estrogen Receptors ? and ? and Kiss1 in the Postnatal Rat Amygdala.

Representative NISSL-stained sections from PND 2 females depicting the anatomical landmarks used to identify each brain region of interest (circled with a solid black solid) and the corresponding sampling template created to define the area of interest (ROI, white dashed line) and quantify the autoradiographic signal within that brain area. An ROI was created for each age and brain area, and then used for all sections, regardless of sex, within that age group. (A) Representative sections containing a midlevel section of the MePD and PLCo, and (B) the AHi and PMCo were obtained from animals in our existing colony to help identify anatomical landmarks in the hybridized tissue used for the present studies. For abbreviations see list. Scale bar = 500 µm.

Jinyan Cao, et al. J Comp Neurol. 2013 February 1;521(2):465-478.
2.
Fig. 6

Fig. 6. From: Sex Specific Expression of Estrogen Receptors ? and ? and Kiss1 in the Postnatal Rat Amygdala.

Representative micrographs showing the deposition of silver grain labeling for Kiss1mRNA in the postnatal mediobasal hypothalamus and amygdala of males (A, C, E) and females (B, D, F). No obvious silver grain clusters were found in the MePD at any age examined, including PND 0 (A and C) and PND 19 (B and D) Although some sections suggested the presence of faint clusters (C and D), their density did not rise above background. Distinct clusters of Kiss1 signal were observed in the ARC on all days examined, however, including PND0 (E) and PND 19 (F) within in the same coronal sections, confirming the success of the labeling. 3V = third ventricle; scale bar = 500 µm for A and B, 50 µm for C to F. Arrow head indicates a Kiss1 silver grain cluster.

Jinyan Cao, et al. J Comp Neurol. 2013 February 1;521(2):465-478.
3.
Fig. 3

Fig. 3. From: Sex Specific Expression of Estrogen Receptors ? and ? and Kiss1 in the Postnatal Rat Amygdala.

Representative autoradiographs depicting ERα mRNA signal in the postnatal rat MePD and PLCo of both sexes (A to C, females on the left side of each panel, males on the right) and silver grain deposits (D to F). Within the MePD and PLCo, labeling was intense in both sexes at birth (A), declined through PND 4 (B), and then returned to PND 0 levels by PND 19 (C) in both sexes (G-H). (D-E) Silver grain deposition confirmed the location of mRNA signal. ERα silver grain deposition is shown in the PND 0 female MePD (D), the PND 4 female MePD (E) and the PND 0 female CoAPL (F). Significant differences in expression compared with PND 0 levels are represented by *P ≤ 0.05 and **P ≤ 0.01 for the females, and #P ≤ 0.05 for the males. The sample size for each group is shown, and the data points represent mean ± SEM. For abbreviations, see list. Scale bar = 1000 µm for A to C, and 50µm for D to F.

Jinyan Cao, et al. J Comp Neurol. 2013 February 1;521(2):465-478.
4.
Fig. 2

Fig. 2. From: Sex Specific Expression of Estrogen Receptors ? and ? and Kiss1 in the Postnatal Rat Amygdala.

Representative autoradiographic images from PND 0 female rats depicting ERα and ERβ mRNA signal in the anterior portion of the neonatal amygdala, and representative images of emulsion dipped slides depicting silver grain deposition in two consecutive sections labeled for ERα (Figure 2 A to F) and ERβ (Figure 2 H to M). ERα silver grain clusters were detected in the MeAD (B), MeAV (C), Ce (D), BA (E) and PLCo (F), but diffusely distributed. ERα signal was also robust in ARC and VMNvl in the same section confirming the specificity and quality of the labeling (A). ERβ signal (H) was readily detectable in the MeAD (I), MeAV (J), Ce (K), BA (L) and Cortex (Cx, M). ERβ mRNA silver grain clusters were densely packed compared to ERα labeling in the same subregions. ERβ signal was also detected in the PVN and VMNvl in the same section (H) confirming the specificity and quality of the labeling. For abbreviations, see list. Scale bar = 500 µm for A and H, and 50µm for B to F and I to M. Arrow heads indicate the silver grain clusters.

Jinyan Cao, et al. J Comp Neurol. 2013 February 1;521(2):465-478.
5.
Fig. 4

Fig. 4. From: Sex Specific Expression of Estrogen Receptors ? and ? and Kiss1 in the Postnatal Rat Amygdala.

Autoradiographs depicting ERα mRNA labeling in the PMCo and AHi of postnatal male and female rats (A–C, females on the left side of each panel, males on the right) on PND 0 (A), PND 4 (B) and PND 7 (C). Representative hemotoxylin-counter stained sections depicting deposition of silver grain labeling for ERα in the PMCo and AHi of PND 0 females (D) and males (E). Expression of ERα mRNA in the PMCo (F) was not dimorphic and declined rapidly in both sexes between PNDs 0 and 2, then remained low through PND 19. (G) ERα expression in the AHi on PND 0 was sexually dimorphic with higher levels in males. Male levels then declined, thus eliminating the sex difference by PND 2. Significant differences in gene expression compared to PND 0 levels are represented by ##P ≤ 0.01 for the males. The significant sex difference is represented by †p ≤ 0.05. The graphs depict mean ± SEM and the sample size is provided for each age. For abbreviations, see list. Scale bar = 1000 µm for A to C, and 100µm for D and E.

Jinyan Cao, et al. J Comp Neurol. 2013 February 1;521(2):465-478.
6.
Fig. 5

Fig. 5. From: Sex Specific Expression of Estrogen Receptors ? and ? and Kiss1 in the Postnatal Rat Amygdala.

Autoradiographs showing ERβ mRNA expression in the postnatal MePD of both sexes (A–E, females on the left side of each panel, males on the right). The pattern of autoradiographic labeling was confirmed by observing silver grain deposition on the emulsion-dipped, counterstained slides (F-K), and the labeling appeared as tight clusters over the counterstained nuclei. ERβ expression was robust and sexually dimorphic at birth with levels higher in females (left panel of A, and F and L) than males (right panel of A, and I and L). This sex difference was lost on PND 2 with decreasing signal in females (B). By PND 4 the sex difference reemerged but was reversed with males having more intense ERβ signal than females (C and L). This difference persisted through PND 7 (D, G, J and L) and PND 19 (E, H, K and L). Temporal expression patterns were sex specific, with signal generally decreasing with age in females, but remaining levels in males (L). Significant differences in expression compared to PND 0 levels are represented by **P ≤ 0.001 for the females. Significant sex differences are represented by †P ≤ 0.05, and ††P ≤ 0.01.The sample size for each age is indicated, and the data points represent mean ± SEM. For abbreviations, see list. Scale bar = 1000 µm for A to E, and 100 µm for F to K.

Jinyan Cao, et al. J Comp Neurol. 2013 February 1;521(2):465-478.

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