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Results: 8

1.
Fig. 4

Fig. 4. From: Reflectance confocal microscopy of optical phantoms.

Calibration of confocal reflectance measurement at 488 nm wavelength, using glass-water(gel) interface. Subsequent measurements of phantom are multiplied by calib (1.204x10-4 V-1) such that a measurement of a glass-water interface will yield Rglass-water = 0.00427 and measurement of a mirror will yield Rglass-water = 1.

Steven L. Jacques, et al. Biomed Opt Express. 2012 June 1;3(6):1162-1172.
2.
Fig. 8

Fig. 8. From: Reflectance confocal microscopy of optical phantoms.

Experimental attenuation vs reflectivity for phantoms at 488 nm wavelength, based on exponential fits of axial profiles (red lines in Fig. 7). The experimental measurement on polystyrene microspheres and the prediction of Mie theory are closely aligned.

Steven L. Jacques, et al. Biomed Opt Express. 2012 June 1;3(6):1162-1172.
3.
Fig. 2

Fig. 2. From: Reflectance confocal microscopy of optical phantoms.

Schematic of the reflectance confocal scanning laser microscope (rCSLM). Laser light is delivered to a focal volume within the phantom, and reflectance from this focus is returned through a pinhole to a detector. The sample stage moves the phantom up and down in order to scan the focus within the phantom.

Steven L. Jacques, et al. Biomed Opt Express. 2012 June 1;3(6):1162-1172.
4.
Fig. 1

Fig. 1. From: Reflectance confocal microscopy of optical phantoms.

The phantoms. (A) Hard BiomimicTM polyurethane phantom, INO, Inc., Canada. (B) Soft BiomimicTM polyurethane phantom, INO. (C) SpectralonTM, Pro-Lite Technology, Inc. Not shown is the gel phantom composed of 1% volume fraction 100-nm-dia. polystyrene microspheres in aqueous agarose gel. (Photos A,B from Ref. 10.)

Steven L. Jacques, et al. Biomed Opt Express. 2012 June 1;3(6):1162-1172.
5.
Fig. 3

Fig. 3. From: Reflectance confocal microscopy of optical phantoms.

Side-view confocal reflectance image in volts, image(z,x) at y = middle of 3D image. The images show log10(voltage), where voltage encodes the reflectance. The color code is black = 0.01 V and white = 10 V. The axial stepsize, Δz, was 1 μm. The microspheres have a glass/gel interface, while others have a water/phantom interface.

Steven L. Jacques, et al. Biomed Opt Express. 2012 June 1;3(6):1162-1172.
6.
Fig. 7

Fig. 7. From: Reflectance confocal microscopy of optical phantoms.

Sampling of 15 axial profiles for the phantoms. The polyurethane phantoms have a baseline of low reflectance at the noise level of the measurement, but there are occasional spikes of reflectance from local strong reflectors. Bold red lines are exponential fits to the data, which is extrapolated to the front surface, indicated by red symbol.

Steven L. Jacques, et al. Biomed Opt Express. 2012 June 1;3(6):1162-1172.
7.
Fig. 6

Fig. 6. From: Reflectance confocal microscopy of optical phantoms.

Calibration grid with experimental data (red circle) and the Mie theory prediction (black diamond) using Eqs. (3), (4) for the polystyrene microsphere gel of Fig. 5. Mie theory predicts μs = 58.2 cm-1, g = 0.129, and Eqs. (3), (4) predict μMIE, = 131 cm‑1, ρMIE = 8.2x10-4. The experiment presents μ = 130 cm‑1, ρ = 9.2x10-4, and μs = 57.7 cm-1, g = 0.072.

Steven L. Jacques, et al. Biomed Opt Express. 2012 June 1;3(6):1162-1172.
8.
Fig. 5

Fig. 5. From: Reflectance confocal microscopy of optical phantoms.

Axial profile of confocal reflectance versus depth of focus, R(zf). The reflectance from the glass-gel interface was assumed to match the specular reflectance of a glass-water interface, Rglass-water = 0.00427, and was used to scale the laboratory counts. Hence, R(zf) is calibrated such that reflectance from a mirror in the focus yields R = 1. The exponential red line is R(zf) = ρ exp(–μ zf). The value ρ is the extrapolated red line value at zf = 0. The value μ is specified by the slope.

Steven L. Jacques, et al. Biomed Opt Express. 2012 June 1;3(6):1162-1172.

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