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Results: 7

1.
Figure 2

Figure 2. From: Small interfering RNA-mediated downregulation of ?-catenin inhibits invasion and migration of colon cancer cells in vitro.

Protein expression of β-catenin in LoVo cells following β-catenin siRNA transfection. LoVo cells were transfected with β-catenin or scrambled siRNA (100 nM). At 48 h after transfection, the protein expression of β-catenin was measured by Western blot assay.

Jianjun Han, et al. Med Sci Monit. 2012;18(7):BR273-BR280.
2.
Figure 1

Figure 1. From: Small interfering RNA-mediated downregulation of ?-catenin inhibits invasion and migration of colon cancer cells in vitro.

mRNA expression of β-catenin in LoVo cells following β-catenin siRNA transfection. LoVo cells were transfected with 100 nM siRNA targeting β-catenin or scrambled siRNA. At 48 h after transfection, mRNA expression of β-catenin was measured by RT-PCR.

Jianjun Han, et al. Med Sci Monit. 2012;18(7):BR273-BR280.
3.
Figure 6

Figure 6. From: Small interfering RNA-mediated downregulation of ?-catenin inhibits invasion and migration of colon cancer cells in vitro.

LoVo cell migration following β-catenin siRNA transfection. Following β-catenin or scrambled siRNA transfection, representative photomicrographs (100×) of LoVo cell migration were taken at 0, 24 and 48 h after wounding. The migration activity was different between LoVo cells with β-catenin siRNA transfection and those with scrambled siRNA transfection.

Jianjun Han, et al. Med Sci Monit. 2012;18(7):BR273-BR280.
4.
Figure 5

Figure 5. From: Small interfering RNA-mediated downregulation of ?-catenin inhibits invasion and migration of colon cancer cells in vitro.

LoVo cell adhesion following β-catenin siRNA transfection. (A) The homogeneity adhesion between LoVo cells transfected with β-catenin siRNA increased significantly as compared to cells with scrambled siRNA transfection; (B) The heterogenicity adhesion of LoVo cells to fibronectin declined significantly after β-catenin siRNA transfection. (* P<0.01 vs. scrambled siRNA, ** P<0.001 vs. scrambled siRNA, n=6).

Jianjun Han, et al. Med Sci Monit. 2012;18(7):BR273-BR280.
5.
Figure 3

Figure 3. From: Small interfering RNA-mediated downregulation of ?-catenin inhibits invasion and migration of colon cancer cells in vitro.

Effects of β-catenin down-regulation on E-cadherin and MMP-7 expressions in LoVo cells. LoVo cells were transfected with β-catenin or scrambled siRNA (100 nM). At 48 h after transfection, the protein expressions of E-cadherin and MMP-7 were deterimined by Western blot assay.

Jianjun Han, et al. Med Sci Monit. 2012;18(7):BR273-BR280.
6.
Figure 4

Figure 4. From: Small interfering RNA-mediated downregulation of ?-catenin inhibits invasion and migration of colon cancer cells in vitro.

(A) CD44v6 expression in LoVo cells following β-catenin siRNA transfection. The white histogram represents isotype control, and black histogram represents CD44v6 at 48 h after transfection. Depicted is the fluorescence intensity vs. cell count. (B) The mean fluorescence intensity of positive staining is shown. (** P<0.001 vs. scrambled siRNA, n=6)

Jianjun Han, et al. Med Sci Monit. 2012;18(7):BR273-BR280.
7.
Figure 7

Figure 7. From: Small interfering RNA-mediated downregulation of ?-catenin inhibits invasion and migration of colon cancer cells in vitro.

LoVo cell invasion following β-catenin siRNA transfection. (A) After incubation for 24 h, invasive cells in scrambled siRNA group and β-catenin siRNA group were stained by crystal violet and photographed under a microscope (400×). (B) Invasion ability was quantitated and data were expressed as histograms. (** P<0.001 vs. scrambled siRNA, n=3).

Jianjun Han, et al. Med Sci Monit. 2012;18(7):BR273-BR280.

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