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Results: 3

1.
Fig. 1.

Fig. 1. From: Genetic and functional analyses implicate the NUDT11, HNF1B, and SLC22A3 genes in prostate cancer pathogenesis.

RNA expression of significantly associated genes in normal prostate tissue of EA, Japanese, and AA individuals. Each distribution is summarized as a boxplot. The horizontal line within the box represents the median of the distribution, and the hinges of the box represent the 25th and 75th percentiles. The P value for each graph denotes the significance of association between expression and genotype. (A) Expression in histologically normal EA tissue (n = 200). (B) Expression in histologically normal Japanese tissue (n = 84). (C) Expression in histologically normal AA tissue (n = 123).

Chiara Grisanzio, et al. Proc Natl Acad Sci U S A. 2012 July 10;109(28):11252-11257.
2.
Fig. 2.

Fig. 2. From: Genetic and functional analyses implicate the NUDT11, HNF1B, and SLC22A3 genes in prostate cancer pathogenesis.

Suppression of risk loci genes has differential effects on cell viability of prostate cell lines. The indicated genes were suppressed by lentivirally mediated delivery of shRNA and viability measured 6 d after selection using Cell Titer Glo (Promega) assay. Specifically, SLC22A3, NUDT11, and HNF1B were each suppressed with two different shRNAs targeting different sites of the genes (Materials and Methods). shRNA targeting luciferase (sh_Luc) was used as a negative control. Data represent the average and SD of at least two independent experiments. Cell viability of LNCaP (A), RWPE (B), and LHSAR (D) cell lines was significantly inhibited by suppression of all targeted candidate genes. Cell viability of PC3 cells (C) was not significantly affected by suppression of targeted candidate genes, compared with Luciferase control.

Chiara Grisanzio, et al. Proc Natl Acad Sci U S A. 2012 July 10;109(28):11252-11257.
3.
Fig. 3.

Fig. 3. From: Genetic and functional analyses implicate the NUDT11, HNF1B, and SLC22A3 genes in prostate cancer pathogenesis.

Suppression of risk loci genes is associated with decreased anchorage-independent growth of prostate cancer cell lines. Soft agar colony formation assays were performed using LNCaP (A) (P values = 0.03, sh_SLC22A3_1; 0.49, sh_SLC22A3_2; 0.01, sh_NUDT11_1; 0.02, sh_NUDT11_2; 0.01, sh_HNF1B_1; 0.03 sh_HNF1B_2; 0.007, sh_MSMB) and PC3 (B) (P values = 0.03, sh_NUDT11_1; 0.01, sh_NUDT11_2; 0.08, sh_HNF1B_1; 0.25 sh_HNF1B_2; 0.02, sh_MSMB) cells that were infected with lentivirus expressing the indicated shRNA. Specifically, SLC22A3, NUDT11, and HNF1B were suppressed with two different shRNAs targeting different sites of the genes (Materials and Methods). shRNA targeting luciferase (sh_Luc) was used as a negative control. shRNA targeting MSMB (sh_MSMB) was used as a positive control, as previously shown to increase anchorage-independent colony formation. P values, paired two-tailed t test. Data represent the average and SD of at least two independent experiments.

Chiara Grisanzio, et al. Proc Natl Acad Sci U S A. 2012 July 10;109(28):11252-11257.

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