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1.
Figure 1

Figure 1. From: Wnt7A Identifies Embryonic γ-Motor Neurons and Reveals Early Postnatal Dependence of γ-Motor Neurons on a Muscle Spindle-Derived Signal.

Wnt7A is a γ-MN marker. A–A″, Wnt7aON cells (A) are located within the ChAT ON motor nucleus (A′) in the lateral ventral horn of P15 mouse spinal cord. A″, Magnification of boxed region in A and A′ showing colabeling of Wnt7a with a subset of ChAT ON cells. B–B″, Small ChAT ON/Wnt7aON cells are interspersed with large ChAT ON/Wnt7aOFF cells at P15. C–C″, Small ChAT ON/Wnt7aON cells express Err3 (arrows) at P15. D–D″, Small Wnt7aON cells express LacZ (arrow) in P21 Gfrα1::LacZ reporter mice. E–E″, Wnt7aON cells exclude NeuN at P21. F–F″, Wnt7aON cells exclude Hb9::GFP at P21. Split and merged channels shown for B–F″. Scale bars: A–A″, 100 μm; B–F″, 20 μm.

Soha Ashrafi, et al. J Neurosci. 2012 June 20;32(25):8725-8731.
2.
Figure 2

Figure 2. From: Wnt7A Identifies Embryonic γ-Motor Neurons and Reveals Early Postnatal Dependence of γ-Motor Neurons on a Muscle Spindle-Derived Signal.

Wnt7A is expressed in embryonic γ-MNs. A–A″, Wnt7a is expressed in the ventricular zone (arrow) and is absent from the ventral motor neuron region in E15.5 Hb9::GFP mice (A, A′). A″, Magnified boxed region in A. B–B″, At E16.5, Hb9::GFP LOW/Wnt7aON cells are interspersed with Hb9::GFP ON/Wnt7aOFF cells in the ventral spinal cord. Wnt7a labeling is also present in the ventricular zone (B, B′). B′, High magnification of boxed region in B. C, C′, E, F, Wnt7aON cells are localized throughout the motor neuron nucleus of E17.5 Hb9::GFP mice and are segregated from Hb9::GFP ON cells. E, Split and merged channels of C′. D, D′, Wnt7aON cells are absent from the ventral spinal cord of E17.5 Gfrα1 −/−; Hb9::GFP mice (D), while Hb9::GFP ON/Wnt7aOFF cells persist (D′). G, H′, Wnt7aON cells in the motor nucleus of E17.5 control mice (G). RetCreERT2/mice lack Wnt7aON cells at E17.5 (H ). ChATON cells in control (G′) and RetCreERT2/mice (H′). Split and merged channels shown for A″, B″, E, and F. Scale bars: A–H′, 100 μm.

Soha Ashrafi, et al. J Neurosci. 2012 June 20;32(25):8725-8731.
3.
Figure 3

Figure 3. From: Wnt7A Identifies Embryonic γ-Motor Neurons and Reveals Early Postnatal Dependence of γ-Motor Neurons on a Muscle Spindle-Derived Signal.

A–C, A spindle-derived GDNF-independent signal is required for γ-MN development. Wnt7aON putative γ-MNs are localized within the motor neuron nucleus of P0 (A), P4 (B), and P15 (C) control mice. D–F, Wnt7a labeling is eliminated in P0 (D), P4 (E), and P15 (F ) Pva::Cre; Isl2::DTA mutant mice. G–I, Wnt7aON γ-MNs are localized within the ventral spinal cord of P0 (G) and P5 (H ) Egr3/mice, but are lost at P15 (I ). J–J−, In control mice, NeuN OFF/ChATON γ-MNs (arrows in J′) intermingle with NeuN ON/ChATON α-MNs at P4. K–K″, NeuN OFF/ChATON γ-MNs are absent in P4 Pva::Cre; Isl2::DTA mice. L–L″, In P0 wild type mice, Wnt7aON putative γ-MNs (arrow) are localized within the Cm motor pool, identified by coexpression of Islet1 and Pea3. Scale bar: A–L″, 100 μm.

Soha Ashrafi, et al. J Neurosci. 2012 June 20;32(25):8725-8731.

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