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1.
Fig. 1

Fig. 1. From: Incorporation of DOPE into Lipoplexes formed from a Ferrocenyl Lipid leads to Inverse Hexagonal Nanostructures that allow Redox-Based Control of Transfection in High Serum.

(A) Structure of BFDMA, a redox-active cationic lipid. The charge of BFDMA can be cycled between +1 (reduced) and +3 (oxidized) by oxidation or reduction of the ferrocenyl groups at the end of each hydrophobic tail; (B) Structure of DOPE; (C) Schematic illustration of a multilamellar vesicle nanostructure; (D) Schematic illustration of a hexagonal nanostructure.

John P. E. Muller, et al. Soft Matter. ;8(24):2608-2619.
2.
Fig. 4

Fig. 4. From: Incorporation of DOPE into Lipoplexes formed from a Ferrocenyl Lipid leads to Inverse Hexagonal Nanostructures that allow Redox-Based Control of Transfection in High Serum.

SAXS spectra obtained using BFDMARED−DOPE (φDOPE = 0.71) (black), BFDMAOX-DOPE (φDOPE = 0.71) (red) or DOPE only (grey) containing lipoplexes in; (A) 1 mM Li2SO4, using lipid concentrations of 0.87 mM BFDMA and/or 2.17 mM DOPE; (B) OptiMEM with 50% (v/v) BS, using diluted lipid concentrations of 0.32 mM BFDMA and/or 0.8 mM DOPE; all in the presence of pEGFP-N1 (2.0 mg/ml) at a charge ratio of 1.1:1 or 3.3:1 (+/−) for reduced or oxidized BFDMA containing solutions respectively. Inserts for both graphs are given on the right hand side. Bragg peaks are indicated on the graphs and color coded to their respective lipoplex.

John P. E. Muller, et al. Soft Matter. ;8(24):2608-2619.
3.
Fig. 5

Fig. 5. From: Incorporation of DOPE into Lipoplexes formed from a Ferrocenyl Lipid leads to Inverse Hexagonal Nanostructures that allow Redox-Based Control of Transfection in High Serum.

Evidence of association of BFDMA and DOPE in solution in the presence and absence of DNA; (A) SANS spectra measured using solutions of; (Δ) 1 mM DOPE (φDOPE = 1); (+) 1 mM BFDMA and 1 mM DOPE (φDOPE = 0.5); (o) 1 mM BFDMA and 0.4 mM DOPE (φDOPE = 0.28), (x) 1 mM BFDMA (φDOPE = 0), all in the presence of DNA (2.9 mg/ml) and, when BFDMA is present, at a charge ratio of 1.1:1 (+/−). The data are offset for clarity. The insert shows an expanded view of the Bragg peaks and has no offset in intensity between samples. (B) SAXS spectra obtained using lipid only solutions of; 1 mM BFDMA (grey); 2.5 mM DOPE (red); 1 mM BFDMA, 2.5 mM DOPE (black); all in 1 mM Li2SO4 (grey).

John P. E. Muller, et al. Soft Matter. ;8(24):2608-2619.
4.
Fig. 3

Fig. 3. From: Incorporation of DOPE into Lipoplexes formed from a Ferrocenyl Lipid leads to Inverse Hexagonal Nanostructures that allow Redox-Based Control of Transfection in High Serum.

Influence of serum on normalized luciferase expression in COS-7 cells treated with lipoplexes formed from pCMV-Luc and mixtures of reduced BFDMA and DOPE for 4 h. The final concentration of BS is given in the legend. DNA was present at a concentration of 2.4 µg/ml for all samples. “DNA” denotes a control with DNA only (no lipid). Molar fractions of DOPE, φDOPE = DOPE/(BFDMA+DOPE), are given on the x-axis for each sample. The concentration of BFDMA in each sample was 8 µM. Luciferase expression was measured 48 h after exposure to lipoplexes. Error bars represent one standard deviation.

John P. E. Muller, et al. Soft Matter. ;8(24):2608-2619.
5.
Fig. 6

Fig. 6. From: Incorporation of DOPE into Lipoplexes formed from a Ferrocenyl Lipid leads to Inverse Hexagonal Nanostructures that allow Redox-Based Control of Transfection in High Serum.

Influence of serum on the normalized luciferase expression in COS-7 cells treated with naked DNA (white bars), and lipoplexes of reduced BFDMA (hashed bars), oxidized BFDMA (black bars), reduced BFDMA and DOPE (φDOPE = 0.71, dotted bars), and oxidized BFDMA and DOPE (φDOPE = 0.71, gray bars) for 4 h. All experiments were performed by adding 50 µL of DNA/lipid mixture in 1 mM Li2SO4 solution to 200 µL media in the presence of cells. The media was pure OptiMEM or a mixture of OptiMEM and BS. The final concentration of BS is indicated along the x-axis. DNA was present at a concentration of 2.4 µg/ml in the presence of cells for all samples. 8 µM BFDMA was present in each BFDMA containing sample. Luciferase expression was measured 48 h after exposure to lipoplexes. Error bars represent one standard deviation.

John P. E. Muller, et al. Soft Matter. ;8(24):2608-2619.
6.
Fig. 2

Fig. 2. From: Incorporation of DOPE into Lipoplexes formed from a Ferrocenyl Lipid leads to Inverse Hexagonal Nanostructures that allow Redox-Based Control of Transfection in High Serum.

Influence of serum on EGFP expression in COS-7 cells treated with lipoplexes formed from pEGFP-N1 and mixtures of reduced BFDMA and DOPE for 4 h. The media used was pure OptiMEM or a mixture of OptiMEM and BS. All experiments were performed by adding 50 µL of lipid/DNA mixture in 1 mM Li2SO4 solution to 200 µL of media in the presence of cells. Final BS concentrations are given down the left hand side of the figure. The overall concentrations of BFDMA and DNA in each lipoplex solution were 8 µM and 2.4 µg/ml, respectively, providing a charge ratio of 1.1:1 (+/−) for all samples as DOPE has a net charge of zero. Mole fractions of DOPE, φDOPE = DOPE/(BFDMA+DOPE), are given across the top of the figure. Fluorescence micrographs (1194 µm by 895 µm) were acquired 48 h after exposure of cells to lipoplexes.

John P. E. Muller, et al. Soft Matter. ;8(24):2608-2619.

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