Results: 5

1.
Figure 5

Figure 5. Comparison of measured concentrations of eotaxin, IL-8 and IP-10 using kits from different vendors. From: Effect of anticoagulants on multiplexed measurement of cytokine/chemokines in healthy subjects.

Results are showing measured concentrations of eotaxin (A), IL-8 (B) and IP-10 (C) detecting in all 4 specimens type either with Bio-rad kits (left panel) or R&D kits (right panel).

Angélique Biancotto, et al. Cytokine. 2012 November;60(2):438-446.
2.
Figure 1

Figure 1. Comparison of measured concentrations for cytokines in serum specimens to all plasma specimens. From: Effect of anticoagulants on multiplexed measurement of cytokine/chemokines in healthy subjects.

Results show concentrations of analytes for all paired individuals (n=11) with the middle bar showing mean concentrations and bars the standard error of the mean.
A) Example of cytokines that yield higher measurements in serum than plasma
B) Example of cytokines that are not detectable in serum compared to plasma
C) Example of cytokines that yield lower measurements in serum than plasma

Angélique Biancotto, et al. Cytokine. 2012 November;60(2):438-446.
3.
Figure 3

Figure 3. Example where measured concentrations of cytokines in plasmaEDTA is different from those in plasmacitrate. From: Effect of anticoagulants on multiplexed measurement of cytokine/chemokines in healthy subjects.

Results show concentrations as histograms of CTACK (A) and IP-10 (B) for all paired individuals (n=11) with error bar representing standard error of the mean. P values shown in the graph are calculated with non-parametric paired Wilcoxon test using plasmacitrate specimens as controls.

Angélique Biancotto, et al. Cytokine. 2012 November;60(2):438-446.
4.
Figure 4

Figure 4. Normalization of measured concentrations of cytokines for Bio-rad kits. From: Effect of anticoagulants on multiplexed measurement of cytokine/chemokines in healthy subjects.

Data were normalized in Bio-plex protocol manager software as Log2 (observed concentration) to normalize data, as log (min) < log (mean) < log (max).
Arrows show cytokines below the detection limit, and yellow circles show 1 sample type that is different from the other 3, easily identifiable by the red/green colors.

Angélique Biancotto, et al. Cytokine. 2012 November;60(2):438-446.
5.
Figure 2

Figure 2. Example of cytokine concentrations in a serum specimen is similar to at least 1 type of plasma specimen. From: Effect of anticoagulants on multiplexed measurement of cytokine/chemokines in healthy subjects.

Results show concentrations as whisker box of analytes for all paired individuals (n=11) with median concentration shown by the line in the middle and the 1th and 99th percentile with the error bar. P values shown in the graph are calculated with non-parametric paired Wilcoxon test using serum specimens as controls.
A) Eotaxin
B) b-NGF
C) SCGF-b

Angélique Biancotto, et al. Cytokine. 2012 November;60(2):438-446.

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