Results: 4

1.
Fig. 4.

Fig. 4. From: Distinct phytochrome actions in nonvascular plants revealed by targeted inactivation of phytobilin biosynthesis.

Expression profiles of red-light-regulated genes in wild type and pubs hy2 double mutant. Log2-normalized fold changes of 1,095 red-light-induced genes and 1,107 red-light-repressed genes obtained from wild-type datasets were calculated. Heat maps of log2-normalized fold changes of these genes in wild type (WT) and pubs hy2 double mutant were shown. The scale of expression is shown in the center.

Yu-Rong Chen, et al. Proc Natl Acad Sci U S A. 2012 May 22;109(21):8310-8315.
2.
Fig. 3.

Fig. 3. From: Distinct phytochrome actions in nonvascular plants revealed by targeted inactivation of phytobilin biosynthesis.

Phototropism of wild type and mutants. The 4-d-old primary protonema germinated from spores was treated with different fluence rates of unilaterally monochromatic red light for 6 d. Primary protonemata showing positive, negative, or no phototropism were counted. For each sample, more than 100 protonemata were scored. Three biological repeats were performed. No primary protonema was negatively phototropic at fluence rates above 1.5 μmol m−2 s−1. Conversely, no primary protonema was positively phototropic at fluence rates below 1.5 μmol m−2 s−1. The values shown are means ± SD.

Yu-Rong Chen, et al. Proc Natl Acad Sci U S A. 2012 May 22;109(21):8310-8315.
3.
Fig. 2.

Fig. 2. From: Distinct phytochrome actions in nonvascular plants revealed by targeted inactivation of phytobilin biosynthesis.

Phenotypes of gene-targeted knockout mutants. (A) Twelve-day-old protonemata of wild type (WT), pubs, hy2 and pubs hy2 grown under white, red and blue light conditions are shown. Bar = 1 mm. (B) Thirty-day-old gametophores of wild type (WT), pubs, hy2 and pubs hy2 grown under white, red and blue light conditions are shown. Gametophores with 7, 9, and 11 recognizable leaves are placed from left to right. (Scale bar: 1 mm.) (CE). Stem length of gametophores from WT and mutants grown under white (C), red (D), and blue (E) light conditions. The length of stems from the shoot apical meristem to the end of the stem was scored. The values shown are means ± SD (n = 10).

Yu-Rong Chen, et al. Proc Natl Acad Sci U S A. 2012 May 22;109(21):8310-8315.
4.
Fig. 1.

Fig. 1. From: Distinct phytochrome actions in nonvascular plants revealed by targeted inactivation of phytobilin biosynthesis.

Time course of BV reduction activity of PUBS. (A) Spectrophotometric time course of BV reduction by PUBS under anaerobic single turnover conditions. Spectra at 1-min intervals are shown. The absorbance decrease at 690 nm, corresponding to the disappearance of BV, and the increase at 492 nm, representing the formation of PUB, are indicated by single arrows. The absorbance change at 580 nm (corresponding to the appearance and disappearance of the two-electron reduced intermediate 15,16-DHBV) is indicated by a double arrow. (B) Reaction mixture aliquots from the steady-state assay of PUBS were withdrawn every 150 s and analyzed by reverse phase HPLC. The integrated HPLC peak areas were plotted as a function of reaction time. Circles indicate biliverdin IXα (BV), open triangles indicate 15,16-dihydrobiliverdin (15,16-DHBV) and squares indicate phycourobilin (PUB).

Yu-Rong Chen, et al. Proc Natl Acad Sci U S A. 2012 May 22;109(21):8310-8315.

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk