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1.
Figure 1

Figure 1. B. melitensis-infected HeLa cells MAPK signaling pathway with mechanistic genes. From: Transcriptome analysis of HeLa cells response to Brucella melitensis infection: A molecular approach to understand the role of the mucosal epithelium in the onset of the Brucella pathogenesis.

This is an example of the DBN model trained with experimental and control expression data showing the mechanistic genes indicated by concentric rings around the node (each node represents a gene and its expression levels). Increasing up regulation is indicated by the gradient colors from light yellow to dark red and down regulation is from light green to dark green. The thickness of the dark green arcs connecting genes (i.e.,IL1R1->CASP2, FGF7->FGFR2) indicate a strong negative correlation relationship while thicker brown arcs indicate a strong positive correlation (i.e., MAPK1->MYC).

Carlos A. Rossetti, et al. Microbes Infect. ;14(9):756-767.
2.
Figure 2

Figure 2. Effect of MAPK-siRNA molecule on the invasion of HeLa cells by Brucella melitensis. From: Transcriptome analysis of HeLa cells response to Brucella melitensis infection: A molecular approach to understand the role of the mucosal epithelium in the onset of the Brucella pathogenesis.

HeLa cells were independently transfected with MAPK1-validated siRNA molecule and 48 h later infected with B. melitensis WT cultures. Non-transfected and cell cultures transfected with siRNA-negative control (siRNA molecules with no homology on eukaryotic genome) were used as controls of infection. The expression of MAPK1 measure by qRT-PCR was knocked-down more than 90% in cells transfected with MAPK1-siRNA molecule. Cell cultures were infected and treated as explained in Materials and Methods. The result suggests the importance of MAPK1 in Brucella invasion to non-professional phagocytic cells. Data are presented as percent of infection and results are the mean + SD (error bars) of 5 independent experiments done in duplicate. Asterisk indicates statistical significant differences (p<0.01) relative to non-transfected cells and cell cultures transfected with siRNA-negative control.

Carlos A. Rossetti, et al. Microbes Infect. ;14(9):756-767.

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