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1.
Figure 3

Figure 3. From: Suppression of Thrombospondin-1 Expression during Uveal Melanoma Progression and its Utilization as Potential Therapeutic.

Inhibition of tumor growth in Tyr-tag mice receiving TSP1-anti-angiogenic mimetic peptide. The 3 week-old Tyr-Tag mice received TSP1 antiangiogenic mimetic peptide or vehicle for 5 weeks (100 mg/Kg/day, 5 days a week) and tumor volumes were evaluated as described in Methods. Histological sections showed a significant decrease in size of tumor in mice receiving the peptide compared to vehicle. The original magnification was x40. The quantification of tumor areas in mice receiving TSP1 peptide indicated that the tumor areas were approximately 10-fold smaller than the tumor areas of mice receiving vehicle (P< 0.05; n=10).

Shoujian Wang, et al. Arch Ophthalmol. ;130(3):336-341.
2.
Figure 2

Figure 2. From: Suppression of Thrombospondin-1 Expression during Uveal Melanoma Progression and its Utilization as Potential Therapeutic.

Suppression of tumor growth in Tyr-Tag mice over expressing TSP1. Tyr-Tag transgenic mice which over-express TSP1 in their eye were generated by crossing Tyr-Tag mice with a line of transgenic mice which express high level of TSP1 in their eyes (Tyr-Tag; TSP1). A slower progression of tumors was observed in Tyr-Tag; TSP1 mice compared to parental Tyr-Tag mice. Histological evaluation of tumors in 8 week-old mice showed a significant decrease in size of tumor in Tyr-Tag; TSP1 mice compared to Tyr-Tag mice. The magnification for the two upper panels was x40 and for the lower panel was x400. The tumor areas were evaluated as described in Methods. Please note that tumor volume in Tyr-Tag; TSP1 mice is approximately 10-fold lower than Tyr-Tag mice (P<0.05; n=10). Although the tumor cells looked similar in two groups, Tyr-Tag; TSP1mice tumors showed less mitotic figures compared to Tyr-Tag mice.

Shoujian Wang, et al. Arch Ophthalmol. ;130(3):336-341.
3.
Figure 1

Figure 1. From: Suppression of Thrombospondin-1 Expression during Uveal Melanoma Progression and its Utilization as Potential Therapeutic.

TSP1 and PECAM-1 staining of frozen eye sections and histological examination of tumors in Tyr-Tag transgenic mice. TSP1 expression in Tyr-Tag tumors from 3-week and 12-week-old mice was examined by immunohistochemistry of frozen eye sections. Strong TSP1 staining was observed in eyes from 3 week-old mice, especially near the site of tumors. However, a significant decrease in TSP1 staining was observed in eyes from 12 week-old mice, where tumor size was increased significantly. The vascularity of tumors was similarly evaluated using anti-PECAM-1, a vascular marker. Although a few blood vessels were observed in eye sections from 3 week-old Tyr-Tag mice, a significant number of blood vessels were visible in section from 12-week-old mice. The H&E staining shows the tumor histology at 3- and 12 weeks. These images (x200) are reprehensive of images evaluated in eyes from at least 10 mice. Arrow heads indicate mitotic figures.

Shoujian Wang, et al. Arch Ophthalmol. ;130(3):336-341.

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