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1.
Figure 6.

Figure 6. From: HDAC1 and HDAC2 are Differentially Expressed in Endometriosis.

Representative pictures of histone deacetylase (HDAC)1-specific intensities scored as strong (3), moderate (2), or weak (1) on the tissue microarray (TMA). ×20 and ×40 pictures are provided for detail.

Maricarmen Colón-Díaz, et al. Reprod Sci. 2012 May;19(5):483-492.
2.
Figure 5.

Figure 5. From: HDAC1 and HDAC2 are Differentially Expressed in Endometriosis.

Proportion of tissues in each histone deacetylase (HDAC)1/2 immunostaining intensity category in endometrial and endometriotic tissues. The proportion of tissues in each tissue type categorized as having strong (3), moderate (2), weak (1), or no (0) immunostaining for HDAC1 and HDAC2 was determined. At the protein level, HDAC1/2 were differentially expressed according to lesion localization, with the highest proportion of positive HDAC1 immunostaining seen in ovarian, skin, and gastrointestinal (GI) lesions, and of HDAC2 in skin and endometria of patients with endometriosis.

Maricarmen Colón-Díaz, et al. Reprod Sci. 2012 May;19(5):483-492.
3.
Figure 1.

Figure 1. From: HDAC1 and HDAC2 are Differentially Expressed in Endometriosis.

Basal gene expression of class I histone deacetylases (HDACs) in human endometrial and endometriotic cell lines. Quantitative polymerase chain reaction (qPCR) quantification of class I HDACs (HDAC1 and HDAC2) was conducted using TaqMan gene expression assays. Results are expressed as mean ± standard deviation (SD) of at least 3 experiments in duplicate. Both HDAC1 and HDAC2 are differentially expressed in human endometrial cell lines. Endometriotic cells express significantly higher levels of HDAC1/2. HESC, endometrial stromal; EEC, endometrial epithelial; ECL, endometriotic cell line. *P < .05.

Maricarmen Colón-Díaz, et al. Reprod Sci. 2012 May;19(5):483-492.
4.
Figure 3.

Figure 3. From: HDAC1 and HDAC2 are Differentially Expressed in Endometriosis.

Gene expression levels of histone deacetylase (HDAC) 1/2 in endometrial and endometriotic tissues. A, Relative gene expression levels in tissues: RNA was isolated from flash-frozen tissues and quantitative polymerase chain reaction (qPCR) was conducted using TaqMan assays. Results are expressed as relative units (RU) after normalization with the reference gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH). The HDAC1 was expressed in tissues of patients with endometriosis (endometriotic and eutopic endometrium), and in the endometria of patients with fibroids. The highest mean expression of HDAC1 was observed in the endometria of patients with endometriosis. Similar results were observed for HDAC2, which was expressed in a substantial proportion of tissues from patients with endometriosis and fibroids. The highest expression of HDAC2 was in endometria from patients with endometriosis. B, Correlations of HDAC1/2 gene expression levels in tissues: a positive and significant correlation was observed in the levels of HDAC1/2 only in endometriotic lesions.

Maricarmen Colón-Díaz, et al. Reprod Sci. 2012 May;19(5):483-492.
5.
Figure 4.

Figure 4. From: HDAC1 and HDAC2 are Differentially Expressed in Endometriosis.

Immunostaining intensity of histone deacetylase (HDAC)1/2 proteins in endometrial and endometriotic tissues. A total of 164 formalin-fixed paraffin-embedded endometrial and endometriotic tissues on a tissue microarray (TMA) were analyzed by immunohistochemistry. Immunostaining intensity of HDAC1 and HDAC2 was evaluated and scored blindly in glands (A and C) and stroma (B and D) by 2 independent observers using the intensity scale (3 = strong, 2 = moderate, 1 = weak, and 0 = no staining). Staining was reported as mean immunohistochemistry (IHC) score ± standard deviation (SD). The highest mean expression of HDAC1 was seen in skin lesions followed by gastrointestinal (GI), ovarian, and endometrium from patients with endometriosis (EE). Differences in mean HDAC2 immunostaining intensity were subtler, with the highest mean expression seen in skin, EE, and GI lesions. The level of expression of HDAC2 in EE was significantly higher than secretory phase endometrium (SE).

Maricarmen Colón-Díaz, et al. Reprod Sci. 2012 May;19(5):483-492.
6.
Figure 2.

Figure 2. From: HDAC1 and HDAC2 are Differentially Expressed in Endometriosis.

Effects of ovarian steroid hormone treatment on histone deacetylase (HDAC) 1/2 gene expression in human endometrial and endometriotic cell lines. Cells were treated for 24 hours with 10−8 mol/L estradiol (E2), 10−7 mol/L progesterone (P4), or E2/P4 for 24 hours. Total RNA was isolated from treated and untreated (medium with vehicle, CV) cells. Quantitative polymerase chain reaction (qPCR) of HDAC1/2 was conducted using TaqMan gene expression assays. Results are expressed as relative expression (relative units, RU) after normalization with the reference gene. The HDAC1 gene expression was significantly downregulated by E2/P4 in endometrial epithelial cells (B) but not in endometrial stromal nor in the endometriotic cells (C). The HDAC2 gene expression levels were significantly increased by E2 and dowregulated by E2 + P4 in endometrial stromal cells (A). **P < .01.

Maricarmen Colón-Díaz, et al. Reprod Sci. 2012 May;19(5):483-492.

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