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1.
Figure 4

Figure 4. From: Human stem cell neuronal differentiation on silk-carbon nanotube composite.

Axonal length on PLO, silk, and silk-CNT composite substrates. Axonal length measurements with β-III tubulin fluorescence images. PLO and silk-CNT substrates demonstrated similar axonal length; however, silk scaffolds induce very limited axonal length growth. Double asterisks, P < 0.001; NS, no statistical significance.

Chi-Shuo Chen, et al. Nanoscale Res Lett. 2012;7(1):126-126.
2.
Figure 7

Figure 7. From: Human stem cell neuronal differentiation on silk-carbon nanotube composite.

hESCs on 3D silk-CNT matrix. Confocal microscopy image of hESC cultured on a silk-CNT scaffold showing β-III tubulin expression in axonal shooting into three-dimensional scaffold matrices. Scale bar, 200 μm.

Chi-Shuo Chen, et al. Nanoscale Res Lett. 2012;7(1):126-126.
3.
Figure 5

Figure 5. From: Human stem cell neuronal differentiation on silk-carbon nanotube composite.

SEM images of hESCs on various substrates. SEM images of (a) cells cultured on PLO exhibiting a flat morphology and two-dimensional axonal connections, (b) cells cultured on silk scaffolds demonstrating three-dimensional structures and cell migration, and (c) cells cultured on silk-CNT scaffolds demonstrating three-dimenstional axonal connections and silk-CNT matrix degradation.

Chi-Shuo Chen, et al. Nanoscale Res Lett. 2012;7(1):126-126.
4.
Figure 3

Figure 3. From: Human stem cell neuronal differentiation on silk-carbon nanotube composite.

Expression level of β-III tubulin and nestin on PLO, silk, and silk-CNT composite substrates. Expression intensity of β-III tubulin and nestin observed with fluorescence microscopy. Silk-CNT scaffolds exhibited maximum β-III tubulin expression, while nestin expression exhibited a similar trend. Single asterisk represents P < 0.01, and double asterisks represent P < 0.001.

Chi-Shuo Chen, et al. Nanoscale Res Lett. 2012;7(1):126-126.
5.
Figure 6

Figure 6. From: Human stem cell neuronal differentiation on silk-carbon nanotube composite.

SEM image of silk-CNT composite. SEM images of PLO, silk, and silk-CNT substrates before cell seeding (A, B, C, respectively) and after incubating with hESCs for 7 days (D, E, F, respectively). On the silk-CNT surface, there were some micro silk-CNT aggregates distributed within silk matrices (C). After 7 days, the silk-CNT substrate became porous. Some neuronal axons were found to extend into those concaves on the substrate (F). Scale bar, 20 μm.

Chi-Shuo Chen, et al. Nanoscale Res Lett. 2012;7(1):126-126.
6.
Figure 2

Figure 2. From: Human stem cell neuronal differentiation on silk-carbon nanotube composite.

Neuronal marker expression. Neuronal marker, β-tubulin III, expression of hESC cultured on (a) PLO exhibiting long two-dimensional axonal development with lower density, (b) silk scaffolds exhibiting some cell migration along with negligible axonal projections, and (c) silk-CNT scaffolds demonstrating three-dimensional axonal elongation as well as cell migration. Scale bar, 200 μm.

Chi-Shuo Chen, et al. Nanoscale Res Lett. 2012;7(1):126-126.
7.
Figure 1

Figure 1. From: Human stem cell neuronal differentiation on silk-carbon nanotube composite.

Silk dispersion and silk-CNT scaffolding. (a) The MWCNT dispersions in DI water (left) and silk fibroin solution (right). MWCNTs form a homogeneous and stable solution without sedimentation in silk fibroin solutions (right); on the contrary, they aggregate into large clusters in water (left). (b) Silk fibroin provides stable matrices to hold the CNTs in aqueous environments. (c) MWCNTs form large aggregates in aqueous solution (stem cell culture media).

Chi-Shuo Chen, et al. Nanoscale Res Lett. 2012;7(1):126-126.

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