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1.
Figure 7

Figure 7. From: Intestinal Mast Cell Levels Control Severity of Oral Antigen-Induced Anaphylaxis in Mice.

Intestinal and systemic manifestations of oral antigen-triggered anaphylaxis in WT and iIL9Tg mice. Temperature change from 0 to 60 minutes and diarrhea in OVA-sensitized, i.g. Veh- or OVA-challenged BALB/c WT and iIL9Tg mice after the seventh OVA challenge. Solid symbols indicate mice that developed diarrhea. *P < 0.05; **P < 0.01.

Richard Ahrens, et al. Am J Pathol. 2012 April;180(4):1535-1546.
2.
Supplemental Figure S1

Supplemental Figure S1. From: Intestinal Mast Cell Levels Control Severity of Oral Antigen-Induced Anaphylaxis in Mice.

Histologic assessment of intestinal mast cells in anaphylactic WT and FceRI−/− mice. Histologic assessment of chloroacetate esterase (CAE)-stained small intestine of OVA-sensitized, i.g. OVA-challenged WT and FceRI−/− mice. Arrows indicate remnants of extracellular mast cell granules (CAE+ granules) in the lamina propria of the small intestine of WT OVA mice, whereas mast cells in FceRI−/− mice remained intact, and granules were confined within the cytoplasm.

Richard Ahrens, et al. Am J Pathol. 2012 April;180(4):1535-1546.
3.
Figure 6

Figure 6. From: Intestinal Mast Cell Levels Control Severity of Oral Antigen-Induced Anaphylaxis in Mice.

Anti-FcγRII/III-mediated passive systemic anaphylaxis in WT and iIL9Tg mice. Maximum temperature change of WT and iIL9Tg mice to i.v. injection of anti-FcγRII/III mAb (clone 2.4G2) or isotype control (Ig, clone J1.2) antibody. Data represent mean ± SEM; n = 4 mice per group from duplicate experiments. **P < 0.01 compared with WT control (Ctrl) Ig. Ctrl Ig, isotype control.

Richard Ahrens, et al. Am J Pathol. 2012 April;180(4):1535-1546.
4.
Figure 8

Figure 8. From: Intestinal Mast Cell Levels Control Severity of Oral Antigen-Induced Anaphylaxis in Mice.

Passive oral antigen-triggered IgE-mediated anaphylaxis in WT and iIL9Tg mice. Maximum temperature change (A), serum Mcpt-1 levels (B), TER (C), and carbachol-induced change in Isc (ΔIsc) (D) of the small intestine of anti-TNP-IgE- (10 μg) or control Ig- (10 μg) treated WT and iIL9Tg mice orally gavaged with TNP-BSA (50 mg/mL; 500 μL). Values represent mean ± SEM; n = 4 to 8 mice per group. *P < 0.05; **P < 0.01. Ctrl Ig, isotype control.

Richard Ahrens, et al. Am J Pathol. 2012 April;180(4):1535-1546.
5.
Figure 5

Figure 5. From: Intestinal Mast Cell Levels Control Severity of Oral Antigen-Induced Anaphylaxis in Mice.

Systemic and intestinal manifestations of anti-IgE-treated WT and iIL9Tg mice. Maximum temperature change (A), cutaneous (ear) and mesenteric leak (B), and lung function (C) in BALB/c WT and iIL9Tg mice treated with isotype control (Ig, clone βGL117) or anti-IgE (clone EM95) antibody. B: Before the anti-IgE treatment, mice received i.v. injection of 2% Evans blue (200 μL/PBS) and were subsequently challenged with anti-IgE. C: WT and iIL9Tg mice received an i.v. anti-IgE injection, and airway reactivity to methacholine was measured by whole-body plethysmography. Number of mice are indicated. Data are presented as mean ± SEM *P < 0.05; **P < 0.01. Ctrl Ig, isotype control; NS, not significant.

Richard Ahrens, et al. Am J Pathol. 2012 April;180(4):1535-1546.
6.
Figure 1

Figure 1. From: Intestinal Mast Cell Levels Control Severity of Oral Antigen-Induced Anaphylaxis in Mice.

Intestinal symptoms in oral antigen-triggered anaphylaxis. A: Oral antigen-triggered anaphylaxis experimental regime. Diarrhea occurrence (B) and mean number of mast cells per high-power field (hpf) (C) in the small intestine of OVA-sensitized, i.g. Veh- or OVA-challenged BALB/c WT mice. The common fractions in panel B represent the number of mice that experienced diarrhea over the number of total mice challenged. TER (D), Isc (E), and β-methylcholine-stimulated (F) changes in Isc (ΔIsc) in jejunum segments of OVA-sensitized, i.g. Veh- or OVA-challenged (seventh challenge) BALB/c WT mice. Values represent mean ± SEM; n = 6 to 10 mice per group. Statistical significance is *P < 0.05 or **P < 0.01.

Richard Ahrens, et al. Am J Pathol. 2012 April;180(4):1535-1546.
7.
Figure 4

Figure 4. From: Intestinal Mast Cell Levels Control Severity of Oral Antigen-Induced Anaphylaxis in Mice.

Correlation between intestinal mast cell levels and intestinal and systemic symptoms of oral antigen-induced anaphylaxis. Spearman's rank correlation coefficient between mean number of mast cells per high-power field (hpf) in the small intestine and small intestine epithelial permeability (TER) (A), or intestinal epithelial chloride secretion (B), and between mean temperature change from 0 to 60 minutes and intestinal epithelial chloride secretion (C), or intestinal epithelial permeability change (D) after the fourth i.g. Veh or OVA challenge in OVA-sensitized WT mice.

Richard Ahrens, et al. Am J Pathol. 2012 April;180(4):1535-1546.
8.
Figure 3

Figure 3. From: Intestinal Mast Cell Levels Control Severity of Oral Antigen-Induced Anaphylaxis in Mice.

Intestinal and systemic manifestations of food-triggered anaphylaxis is FcεRI/IgE dependent. Temperature change from 0 to 60 minutes and diarrhea (A) and mast cells per high-power field (HPF) in small bowel in WT and FceRI−/− mice (B) after the seventh OVA challenge. Solid symbols indicate mice that developed diarrhea. C: Lung function in OVA-sensitized, i.g. Veh- or OVA-challenged WT and FceRI−/− mice. Airway reactivity to methacholine was measured 30 minutes after the seventh Veh or OVA challenge with the use of whole-body plethysmography. Data in B and C are represented as mean ± SEM; n = 4 to 8 mice per group from duplicate experiments. *P < 0.05; **P < 0.01. NS, not significant.

Richard Ahrens, et al. Am J Pathol. 2012 April;180(4):1535-1546.
9.
Figure 2

Figure 2. From: Intestinal Mast Cell Levels Control Severity of Oral Antigen-Induced Anaphylaxis in Mice.

Systemic manifestations in oral antigen-triggered anaphylaxis. A: Before the seventh OVA challenge, mice received i.v. injection of 2% Evans Blue (200 μL/PBS) and were subsequently challenged with OVA. Sixty minutes after OVA challenge, the mice were sacrificed, and Evans blue concentration was determined in the ear (cutaneous) and intestine (mesenteric). Temperature change from 0 to 60 minutes (B), lung function (C), histology (D), and BALF Mcpt-1 levels (E) after the seventh i.g. Veh or OVA challenge in OVA-sensitized WT mice. C: Airway reactivity to methacholine was measured 30 minutes after the seventh OVA challenge with the use of whole-body plethysmography and invasive technique (flexiVent system). D: Original magnification, ×40. Data in A and C are represented as mean ± SEM; n = 4 to 8 mice per group from triplicate experiments. *P < 0.05; **P < 0.01.

Richard Ahrens, et al. Am J Pathol. 2012 April;180(4):1535-1546.

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