Results: 3

1.
Fig. 3

Fig. 3. From: Mitochondrial ATP-sensitive potassium channel activity and hypoxic preconditioning are independent of an inwardly rectifying potassium channel subunit in C. elegans.

C. elegans mKATP channel activity is independent of the Kir family gene products irk-1, -2, and -3. C. elegans mKATP channel activity in mitochondrial purified from N2-Bristol control worms (WT; white bars) or the irk-1(n4895);irk-2(n4896);irk-3(n5049) triple mutant (triple; gray bars). The baseline Δ fluorescence (Ctrl, set to 100%) was 15.3 ± 3.0 and 15.8 ± 2.7 arbitrary units for the WT and triple mutant, respectively. Pharmacologic agents were present as listed below the x-axis. Abbreviations: AA5, Atpenin A5; DZX, diazoxide; Gly, Glyburide; 5HD, 5-hydroxydecanoate. Data are means ± SEM, N>4 (N=independent mitochondria preparations). #p<0.05 vs. Ctrl., *p<0.05 vs. ATP, †p<0.05 vs. ATP+DZX or ATP+AA5.

Andrew P. Wojtovich, et al. FEBS Lett. ;586(4):428-434.
2.
Fig. 1

Fig. 1. From: Mitochondrial ATP-sensitive potassium channel activity and hypoxic preconditioning are independent of an inwardly rectifying potassium channel subunit in C. elegans.

Expression analysis of the irk genes in C. elegans. The promoter and genomic ORFs of irk-1 (A–F) and irk-2 (G–L) and the promoter of irk-3 (M–P) were amplified via PCR, fused to the ORF of GFP, and micro-injected to create transgenic strains. Representative fluorescent confocal (A, C, E, G, I, K, M, and O) and Normarski (B, D, F, H, J, L, N, and P) images are shown indicating the cells where the promoter is active. The irk-1 and irk-2 translational fusions additionally demonstrate plasma membrane localization of the respective fusion proteins. The worms in panels C and D are male (♂). Abbreviations: ag, anterior ganglia; AF, intestinal autofluorescence; bwm, body wall muscle; gon, gonad; gsc, gonadal sheath cell; HSN, hermaphrodite specific neurons; int, intestine; mec, mechanosensory neurons; pg, posterior ganglia; phs, phasmid neurons; piv, pharyngeal intestinal valve cells; spt, spermatheca; ut, uterus.

Andrew P. Wojtovich, et al. FEBS Lett. ;586(4):428-434.
3.
Fig. 2

Fig. 2. From: Mitochondrial ATP-sensitive potassium channel activity and hypoxic preconditioning are independent of an inwardly rectifying potassium channel subunit in C. elegans.

HP in C. elegans is independent of the Kir family gene products irk-1, -2, and -3. (A) C. elegans WT control (N2-Bristol), and mutant deletion strains containing either the irk-1(n4895)X, irk-2(n4896)X, or irk-3(n5049)X alleles or all three mutant alleles in combination (triple) were subjected to simulated ischemia reperfusion (IR; white squares) or hypoxic preconditioning plus IR (HP+IR, gray squares) as detailed in the methods. Viability is expressed as percent of dead worms. To minimize handling artifacts, paired trials were performed on the same day using worms of identical age, and the IR and HP+IR values for each paired trial are linked together in the plot. (B) In order to compare different mutants, the degree of protection afforded by HP (equivalent to the IR minus the HP+IR for each paired trial) was averaged across the seven trials for each genotype. Means ± SEM, N=7, (N = independent trials performed on separate days, with each trial consisting of the average value obtained from 3 plates of worms, with >50 worms per plate), *p<0.05 vs. IR.

Andrew P. Wojtovich, et al. FEBS Lett. ;586(4):428-434.

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