Results: 5

1.
Figure 4

Figure 4. From: Biomineralization and Size Control of Stable Calcium Phosphate Core Protein Shell Nanoparticles: Potential for Vaccine Applications.

SEM imaging of CaP structures mineralized in the absence of protein (A), or in the presence of wild type TrxA (B), TrxA::PA44 (C), and TrxA::PA44C32S. The inset of panels C and D show higher resolution images.

David Chiu, et al. Bioconjug Chem. ;23(3):610-617.
2.
Figure 1

Figure 1. From: Biomineralization and Size Control of Stable Calcium Phosphate Core Protein Shell Nanoparticles: Potential for Vaccine Applications.

Characterization of CaP chips. Non-annelaed (A–B) and annealed samples (C–D) were imaged by SEM at two different magnifications. Panel E shows XRD spectra for both samples. Hydroxyapatite powder was used as a control. Reflections corresponding to CaP phases and the underlying Ti substrate are labeled.

David Chiu, et al. Bioconjug Chem. ;23(3):610-617.
3.
Figure 5

Figure 5. From: Biomineralization and Size Control of Stable Calcium Phosphate Core Protein Shell Nanoparticles: Potential for Vaccine Applications.

Anti-TrxA IgG titers in the serum of C57BL/6 mice was measured by ELISA assay 9 and 21 days post-challenge with TrxA::PA44 and alum or TrxA::PA44-stabilized nanoparticles. Titers for individual animal and cohort means (horizontal bars) are shown. Data are corrected for pre-immune backgrounds. Confidence levels are 84.6% for 9 days data and 98.5% for 21 days data.

David Chiu, et al. Bioconjug Chem. ;23(3):610-617.
4.
Figure 3

Figure 3. From: Biomineralization and Size Control of Stable Calcium Phosphate Core Protein Shell Nanoparticles: Potential for Vaccine Applications.

TEM analysis of nanoparticles produced in the presence of TrxA::PA44. High (A) and low resolution (B) TEM images show that the characteristic size and polydispersity of CaP nanoparticles obtained under our experimental conditions. Selected area electron diffraction (SAED) patterns (C) were diffuse with no distinguishable spots or rings, suggesting that the mineral is amorphous calcium phosphate (ACP).

David Chiu, et al. Bioconjug Chem. ;23(3):610-617.
5.
Figure 2

Figure 2. From: Biomineralization and Size Control of Stable Calcium Phosphate Core Protein Shell Nanoparticles: Potential for Vaccine Applications.

Purification of TrxA::PA44 by heat shock and ion exchange chromatography (IEC). Lanes: M, markers; WC, whole cells; Sol and Ins: soluble and insoluble fractions before and after 10 min incubation at 80°C; IEC, ion exchange chromatography purification of heat-shocked soluble fractions. The position of the molecular mass markers (lane M) are indicated in kDa on the left of the gel which was silver-stained to reveal purified protein and contaminants.

David Chiu, et al. Bioconjug Chem. ;23(3):610-617.

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