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1.
Fig 4

Fig 4. From: Failure of the Smallpox Vaccine To Develop a Skin Lesion in Vaccinia Virus-Na?ve Individuals Is Related to Differences in Antibody Profiles before Vaccination, Not After.

t test comparisons of d28 − d0 (Δ) signals of no-take responders versus takes. t tests were performed as described in the legend to Fig. 3. (A) All no-take responders (n = 8) versus naïve takes (n = 50); (B) no-take responders receiving undiluted DryVax only (n = 4) versus takes (n = 50). R+, responders only.

Xiaolin Tan, et al. Clin Vaccine Immunol. 2012 March;19(3):418-428.
2.
Fig 7

Fig 7. From: Failure of the Smallpox Vaccine To Develop a Skin Lesion in Vaccinia Virus-Na?ve Individuals Is Related to Differences in Antibody Profiles before Vaccination, Not After.

Recombinant vaccinia virus protein ELISAs. OD450 values for pre- and postvaccination sera (diluted to 1/150) from the three donor groups and assayed against six different recombinant vaccinia virus antigens by ELISA. The individual donors within each group are ranked from left to right by ascending vaccine dose. ND, not done.

Xiaolin Tan, et al. Clin Vaccine Immunol. 2012 March;19(3):418-428.
3.
Fig 5

Fig 5. From: Failure of the Smallpox Vaccine To Develop a Skin Lesion in Vaccinia Virus-Na?ve Individuals Is Related to Differences in Antibody Profiles before Vaccination, Not After.

t test comparisons of d0 signals in no-takes versus takes. t tests were performed as described in the legend to Fig. 3. (A) All no-takes (n = 15) versus takes (n = 50); (B) no-take responders only (n = 8) versus takes (n = 50); (C) no-take responders only (n = 8) versus previously vaccinated takes. Horizontal dashed line, cutoff defined as the mean + 2 SD of d0 signals in the naïve/take population. R+, responders only; SA, signature antigen.

Xiaolin Tan, et al. Clin Vaccine Immunol. 2012 March;19(3):418-428.
4.
Fig 6

Fig 6. From: Failure of the Smallpox Vaccine To Develop a Skin Lesion in Vaccinia Virus-Na?ve Individuals Is Related to Differences in Antibody Profiles before Vaccination, Not After.

Comparisons of antibody titers of naïve no-takes versus naïve takes by WR113/D8L ELISA. (A) Individual donor endpoint titers at d0 and d28 postvaccination are plotted for no-takes (n = 15) and takes (n = 17). Vaccine dilution received is indicated below each sample, and responders (as defined by arrays in Table 2) are indicated by asterisks. Horizontal dashed line, average d0 titer; horizontal solid line, average d28 titer. (B) P values for different t test comparisons using ELISA data. Each test was performed with naïve takes (n = 50) versus all no-takes (n = 15) or no-take responders only (R+; n = 8).

Xiaolin Tan, et al. Clin Vaccine Immunol. 2012 March;19(3):418-428.
5.
Fig 2

Fig 2. From: Failure of the Smallpox Vaccine To Develop a Skin Lesion in Vaccinia Virus-Na?ve Individuals Is Related to Differences in Antibody Profiles before Vaccination, Not After.

Dose-response of antibody profiles. (A) The average signals on d28 − d0 (Δ) of six signature antigens for 23 individuals without a take (see Fig. 1A) were plotted against vaccine dilution received. Day 0 values were subtracted from d28 values to account for various backgrounds. (B) Heat map of the six signature antigens for the 23 no-takes, ranked from left to right by vaccine dilution. Maps for prevaccination (d0), postvaccination (d28), and d28 − d0 (Δ) signals are shown separately. Responders, as defined in Table 2 (n = 8), are indicated by an asterisk. Vaccinees that received APSV are indicated by a plus sign.

Xiaolin Tan, et al. Clin Vaccine Immunol. 2012 March;19(3):418-428.
6.
Fig 3

Fig 3. From: Failure of the Smallpox Vaccine To Develop a Skin Lesion in Vaccinia Virus-Na?ve Individuals Is Related to Differences in Antibody Profiles before Vaccination, Not After.

t test comparison of antibody profiles in no-take responders receiving undiluted versus diluted smallpox vaccine. t tests were performed comparing undiluted vaccine profiles (n = 4) to profiles obtained with vaccine dilutions of 1/10, 1/5, and 1/3 (n = 4) using log-transformed and normalized data. Benjamini and Hochberg-corrected P values (pBH) are overlaid onto histograms generated from retransformed average signal intensities ± standard errors of the means (SEM). Significant differences are indicated by pBH values of <0.05, nonsignificant differences are ≥0.05. (A) d28 profiles; (B) d28 − d0 (Δ) profiles.

Xiaolin Tan, et al. Clin Vaccine Immunol. 2012 March;19(3):418-428.
7.
Fig 1

Fig 1. From: Failure of the Smallpox Vaccine To Develop a Skin Lesion in Vaccinia Virus-Na?ve Individuals Is Related to Differences in Antibody Profiles before Vaccination, Not After.

Heat map overview of the antibody profiles analyzed in this study. Serum samples are listed horizontally, and arrayed antigens are listed vertically. Sera from three groups of vaccinated donors, obtained prior to vaccination (d0) and 28 days after receiving DryVax or APSV (d28), were probed against arrays. (A) Vaccinia-naïve individuals who failed to develop a take after vaccination (n = 23); (B) vaccinia-naïve individuals who developed a take after vaccination (n = 50); (C) previously vaccinated individuals who developed a take after vaccination (n = 25). Vaccine dilutions are indicated immediately above the heat map. Donors are ranked from left to right by ascending average signal intensity. The arrayed antigens comprise each individual vaccinia virus ORF in the WR genome expressed from T7 plasmids in coupled in vitro transcription/translation (IVTT) reactions. Each antigen is designated (right) by the WR number followed by the Copenhagen nomenclature. Only the top 25 most reactive antigens are shown. Control spots comprise IVTT lacking plasmid template (No DNA; used for data normalization), purified human IgG used as a control for the secondary antibody, and purified Epstein-Barr virus nuclear antigen-1 (EBNA-1) used as a control for serum quality. The heat map was generated from log-transformed and normalized data using vsn, and the data were retransformed back into approximate raw signal intensities for the heat map and converted to a color scale. *, pseudogene.

Xiaolin Tan, et al. Clin Vaccine Immunol. 2012 March;19(3):418-428.

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