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Results: 4

1.
Figure 1

Figure 1. From: The role of pDC, recipient Treg and donor Treg in HSC engraftment.

FC are a heterogeneous population. FC from bone marrow are CD8+/TCR on flow cytometric analysis (FC TOTAL). In this heterogeneous FC TOTAL population, the majority are p-preDC. The majority of subpopulation is B220+/CD11b/CD11c+ p-preDC FC. Other subpopulations include CD19+ FC and NK1.1+/DX5+ FC.

Paul A Cardenas, et al. Chimerism. 2011 Jul-Sep;2(3):65-70.
2.
Figure 4

Figure 4. From: The role of pDC, recipient Treg and donor Treg in HSC engraftment.

p-preDC are the key subpopulation in the FC. B10 mice were conditioned with 950 cGy TBI and transplanted with HSC alone or in combination with FC total, p-preDC FC or FC without p-preDC from B10.BR mice. The % survival of recipients is represented by the Kaplan-Meier method.

Paul A Cardenas, et al. Chimerism. 2011 Jul-Sep;2(3):65-70.
3.
Figure 2

Figure 2. From: The role of pDC, recipient Treg and donor Treg in HSC engraftment.

The role of IDO in tolerance. Interferon and CTLA-4 plus CD80/CD86 ligation induce IDO DC toward IDO+ DC. The change in the microenvironment with depletion of tryptophan and increased production of kynurenine produce a change in T cells, T naïve cells differentiate toward Treg. Treg suppress the proliferation of CD8+, CD4+ T cells and NK cells, and enhance engraftment of HSC.

Paul A Cardenas, et al. Chimerism. 2011 Jul-Sep;2(3):65-70.
4.
Figure 3

Figure 3. From: The role of pDC, recipient Treg and donor Treg in HSC engraftment.

Mechanism of tolerance induction by FC: FC induce antigen-specific Treg in vivo. A competitive HSC repopulation assay was used to test the specificity of Treg in vivo. Sorted B6 FC and B6 HSC were transplanted into primary NOD mouse recipients conditioned with 950 cGy TBI (A. primary transplant). CD4+/CD25bright chimeric Treg were sorted from the spleens of five weeks mixed chimeras (B6→NOD). Chimeric Treg were transplanted with HSC from donor (B6) and third party (B10.BR) into conditional secondary recipients (NOD) (B. secondary transplant). Only the donor B6 HSC engrafted, demonstrating antigen-specificity of the “chimeric” Treg.

Paul A Cardenas, et al. Chimerism. 2011 Jul-Sep;2(3):65-70.

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