We are sorry, but NCBI web applications do not support your browser and may not function properly. More information

Results: 4

1.
Figure 2

Figure 2. Decreased Aβ accumulation in human APOE haploinsufficient mice. From: Haploinsufficiency of human APOE reduces amyloid deposition in a mouse model of A? amyloidosis.

Cortical tissues from 3-month-old APOE homozygous and hemizygous mice were sequentially homogenized with PBS and RIPA. Aggregated forms of Aβ in the RIPA-insoluble pellet were solublized with 5M guanidine HCl buffer. A, B, RIPA-insoluble Aβ40 (A) and Aβ42 levels (B) were measured from APOE3/3 and APOE3/− mice. C, D, Similarly, RIPA-insoluble Aβ40 (C) and Aβ42 levels (D) were measured from APOE4/4 and APOE4/− mice. (n=12–20 per genotype)

Jungsu Kim, et al. J Neurosci. ;31(49):18007-18012.
2.
Figure 3

Figure 3. Haploinsufficiency of human APOE leads to reduction of Aβ and fibrillar Aβ plaque deposition. From: Haploinsufficiency of human APOE reduces amyloid deposition in a mouse model of A? amyloidosis.

A, B, D, E, Brain sections from 3-month-old APOE homozygous (A, D) and hemizygous (B, E) mice were immunostained for amyloid with anti-Aβ antibody (HJ3.4-biotin). Scale bar: 400μm. A, B, C, The extent of Aβ deposition detected by HJ3.4-biotin antibody was quantified (C) from cortex of APOE3/3 (A) and APOE3/− mice (B). D, E, F, The extent of Aβ deposition detected was quantified (F) from cortex of APOE4/4 (D) and APOE4/− mice (E). G, H, J, K, Brain sections from 3-month-old APOE homozygous (G, J) and hemizygous (H, K) mice were stained with X-34 dye that recognizes only fibrillar plaques. Scale bar: 200μm. G, H, I, Fibrillar plaque load detected by X-34 dye was quantified (I) from cortex of APOE3/3 homozygous (G) and APOE3/− mice (H). J, K, L, The extent of fibrillar plaque load was also analyzed (L) from cortex of APOE4/4 (J) and APOE4/− mice (E). (n=12–20 per genotype)

Jungsu Kim, et al. J Neurosci. ;31(49):18007-18012.
3.
Figure 1

Figure 1. Reduction of apoE levels in human APOE haploinsufficient mice. From: Haploinsufficiency of human APOE reduces amyloid deposition in a mouse model of A? amyloidosis.

Cortex from APOE homozygous (APOE3/3 and APOE4/4) and hemizygous (APOE3/− and APOE4/−) mice were used to measure apoE mRNA and protein levels. A, ApoE mRNA levels were measured by quantitative real-time PCR. B, C, Levels of PBS-soluble apoE were assessed by probing a membrane with anti-apoE antibody (B). Quantitative analyses of western blots were performed with tubulin normalization (C) (n=4 per genotype). C, D, To validate the western blot data, apoE levels were also measured by using an apoE-specific ELISA from APOE3/3 and APOE3/− mice (C) and APOE4/4 and APOE4/− mice (D) (n=9–18 per genotype). E, Levels of APP, APP CTFs, PS1 NTF, and BACE1 proteins were measured by Western blotting (n=4 per genotype). Levels of these proteins after normalizing with tubulin signals were not significantly altered by APOE haploinsufficiency. All graphs represent values in mean ± SEM.

Jungsu Kim, et al. J Neurosci. ;31(49):18007-18012.
4.
Figure 4

Figure 4. Attenuation of microgliosis in human APOE haploinsufficient mice. From: Haploinsufficiency of human APOE reduces amyloid deposition in a mouse model of A? amyloidosis.

A, B, D, E, Brain sections from 3-month-old male APOE homozygous (A, D) and male hemizygous (B, E) mice were immunostained with an antibody against activated microglial CD45. A, B, C, The percent area covered by CD45 staining was quantified (C) from the cortex of APOE3/3 (A) and APOE3/− mice (B). D, E, F, The extent of microgliosis was also quantified (F) from the cortex of APOE4/4 (D) and APOE4/− mice (E). Scale bar: 200μm for lower magnification and 90μm for higher magnification. (n=6–12 male mice per genotype). G, Levels of synaptophysin, glutamate receptor (GluR) 2/3/4, NDMA receptor 2b, and postsynaptic density protein 95 (PSD95) levels were measured by Western blotting (n=4 per genotype). Levels of these proteins after normalizing with tubulin signals were not significantly altered by APOE genotype or haploinsufficiency.

Jungsu Kim, et al. J Neurosci. ;31(49):18007-18012.

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk