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1.
Fig. 6.

Fig. 6. From: Ets transcription factor Pointed promotes the generation of intermediate neural progenitors in Drosophila larval brains.

Ectopic expression of Yan activates ase expression in type II NBs and eliminates INPs in type II NB lineages. (A and A′) A wild-type third-instar larval brain in which type II NB lineages are labeled with mCD8-RFP driven by insc-Gal4 ase-Gal80 and R9D11-mCD8-GFP. Arrows indicate type II NBs. (B and B′) Expression of Yan driven by Insc-Gal4 ase-Gal80 leads to ectopic expression of Ase in type II NBs (arrows) and elimination of R9D11-mCD8-GFP-positive INPs. Instead, only GMCs (arrowheads) are produced. (C and D) Quantification of the number of Ase type II NBs (C) and mature INPs (D). *P < 0.01 (Student t test).

Sijun Zhu, et al. Proc Natl Acad Sci U S A. 2011 December 20;108(51):20615-20620.
2.
Fig. 5.

Fig. 5. From: Ets transcription factor Pointed promotes the generation of intermediate neural progenitors in Drosophila larval brains.

pntΔ33 mutant type II NB lineages have reduced numbers of INPs and GMCs. (A–B′′) A wild-type (A–A′′) and pntΔ33 mutant (B–B′′) type II NB clones at the wandering larval stage. Clones are labeled with mCD8-GFP driven by elav-Gal4 and outlined by dashed lines. Only a single focal plane is shown for each clone for clarity. Asterisks indicate NBs. Arrows point to mature INPs and arrowheads to GMCs. (C–E) Quantification of the number of immature INPs (C), mature INPs (D), and GMCs (E). n = 12 in each sample. *P < 0.01 (Student t test).

Sijun Zhu, et al. Proc Natl Acad Sci U S A. 2011 December 20;108(51):20615-20620.
3.
Fig. 4.

Fig. 4. From: Ets transcription factor Pointed promotes the generation of intermediate neural progenitors in Drosophila larval brains.

Ectopic PntP1 expression results in overproliferation of type II NB-like cells in type I NB lineages in the absence of Brat. (A) A wild-type third-instar larval brain lobe contains eight type II NBs (arrows, only six are shown) on the dorsal side. (B) A wild-type VNC contains only type I NB lineages (e.g., arrowheads). (C) Ectopic expression of PntP1 suppresses Ase expression in type I NBs (e.g., arrows) and promotes the generation of INP-like cells (e.g., arrowheads) in VNCs, but does not result in overproliferation of NBs. (D) Knockdown of Brat by RNAi leads to over-proliferation of Dpn+ Ase type II NB-like cells on the dorsal side of larval brains (e.g., arrows). (E) Knockdown of Brat by RNAi has no obvious phenotype in type I NB lineages (e.g., arrowheads) in VNCs. (F) Simultaneous expression of PntP1 and brat RNAi leads to numerous Dpn+ Ase type II NB-like cells in VNCs (e.g., arrows).

Sijun Zhu, et al. Proc Natl Acad Sci U S A. 2011 December 20;108(51):20615-20620.
4.
Fig. 3.

Fig. 3. From: Ets transcription factor Pointed promotes the generation of intermediate neural progenitors in Drosophila larval brains.

PntP1-induced ectopic INP-like cells express the INP-specific marker R9D11-mCD8-GFP and require the suppression of Ase in the NB for their generation. (A and A′) R9D11-mCD8-GFP specifically labels INPs in type II NB lineages on the dorsal side of larval brains. Insets in (A–A′′) show an enlarged view of the lineage highlighted in a dashed square. Arrows point to type II NBs and arrowheads to immature INPs. (B and B′′) R9D11-mCD8-GFP is not expressed in type I NB lineages on the ventral side of larval. (C and C′) PntP1 induces ectopic expression of R9D11-mCD8-GFP in type I NB lineages that contain INP-like cells on the ventral side of larval brains. The enlarged view (Insets) of the lineage highlighted by a dashed square shows that ectopic R9D11-mCD8-GFP expression is only detected in Ase+ mature INP-like cells but not the NB (arrow) or Ase immature INP-like cells (arrowheads). (D and D′) Expression of Ase eliminates PntP1-induced ectopic R9D11-mCD8-GFP–positive INP-like cells in type I NB lineages.

Sijun Zhu, et al. Proc Natl Acad Sci U S A. 2011 December 20;108(51):20615-20620.
5.
Fig. 2.

Fig. 2. From: Ets transcription factor Pointed promotes the generation of intermediate neural progenitors in Drosophila larval brains.

Expression of PntP1 in type I NB lineages suppresses Ase expression in NBs and promotes the generation of ectopic INP-like cells. (A and A′) The ventral side of a wild-type third-instar larval brain contains only type I NBs (e.g., arrows). (Insets) An enlarged view of a type I NB lineage highlighted by a dashed square. Each type I NB lineage has a single Ase+ Dpn+ NB (arrows) and several Ase+ Dpn GMCs (arrowheads). (B and B′) Ectopic expression of PntP1 suppresses Ase in type I NBs (e.g., arrows) and promotes the generation of INP-like cells in a subset of type I NB lineages (outlined by dotted circles). (Insets) An enlarged view of a lineage (highlighted by a dashed square) that contains an Ase Dpn+ NB (arrows), two Ase Dpn immature INP-like cells (large arrowheads, only one is shown), several Ase+ Dpn+ mature INP-like cells (e.g., small white arrowheads), and Ase+ Dpn GMCs (e.g., yellow arrowheads). (C and C′) A wild-type type I NB clone in the VNC contains an Ase+ Dpn+ NB (arrows). (D and D′) Expression of PntP1 in a type I NB clone in the VNC suppresses Ase expression in the NB (arrows) and induces the generation of multiple Ase+ Dpn+ mature INP-like cells (white arrowheads), as well as Ase+ Dpn GMCs (yellow arrowheads).

Sijun Zhu, et al. Proc Natl Acad Sci U S A. 2011 December 20;108(51):20615-20620.
6.
Fig. 1.

Fig. 1. From: Ets transcription factor Pointed promotes the generation of intermediate neural progenitors in Drosophila larval brains.

PntP1 is specifically expressed in type II NB lineages in third instar larval brains. (A–A′′) Type II NB lineages are specifically labeled by the expression of mCD8-GFP driven by insc-Gal4 ase-Gal80 on the dorsal side of a larval brain. (Insets) An enlarged view of a single type II NB lineage. Type II NB lineages are outlined by dotted circles. Arrows point to Ase Dpn+ type II NBs. Only five of eight type II NBs are shown in this particular focal plane. Large arrowheads in Insets indicate Ase Dpn immature INPs and small arrowheads point to Ase+ Dpn+ mature INPs. (B–B′′) mCD8-GFP driven by Gal414-94 labels type II NB lineages. Dotted circles outline type II NB lineages and arrows point to type II NBs as in A–A′′. Only seven type II NB lineages are shown. (C) A schematic diagram showing the genomic insertion site of Gal414-94. (D and D′) Immunostaining of PntP1 shows PntP1 is expressed in type II (dotted circles) but not in type I NB (dashed circles) lineages. (EF′) Two focal planes of PntP1 staining in a single type II NB lineage. The NB is outlined by a dashed circle. Arrows point to Ase immature INPs, large arrowheads to newly mature Ase+ INPs, and small arrowheads to late mature INPs. OL: optic lobe in this and following figures.

Sijun Zhu, et al. Proc Natl Acad Sci U S A. 2011 December 20;108(51):20615-20620.

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