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1.
Figure 6.

Figure 6. From: Three-Dimensional Microstructural Changes in Murine Abdominal Aortic Aneurysms Quantified Using Immunofluorescent Array Tomography.

Organizational analysis: elastin fragmentation. In the elastase group only, elastin fragmentation increased significantly over time, with day 28 elastin fragmentation being significantly higher than both day 0 and day 7. Mean ± SEM, N=20, n=4 per group.

Sanaz Saatchi, et al. J Histochem Cytochem. 2012 February;60(2):97-109.
2.
Figure 7.

Figure 7. From: Three-Dimensional Microstructural Changes in Murine Abdominal Aortic Aneurysms Quantified Using Immunofluorescent Array Tomography.

Cellular analysis: nuclei amount. The amount of nuclei increased across all time points in the elastase group. In the elastase group only, the number of nuclei at day 28 was significantly higher than that at day 0 and day 7. Mean ± SEM, N=20, n=4 per group.

Sanaz Saatchi, et al. J Histochem Cytochem. 2012 February;60(2):97-109.
3.
Figure 1.

Figure 1. From: Three-Dimensional Microstructural Changes in Murine Abdominal Aortic Aneurysms Quantified Using Immunofluorescent Array Tomography.

Aneurysmal aortic dilation. Infrarenal aortic external diameter was measured to calculate aortic dilation after elastase and heat-inactivated elastase treatment. At day 28, aortic dilation was significantly larger (*p<0.01) in the elastase-treated group than in the heat-inactivated elastase-treated group. The day 28 elastase group was considered aneurysmal due to an increase in aortic diameter greater than 100% from the pre-elastase treatment time point. Mean ± SEM, N=20, n=4 per group.

Sanaz Saatchi, et al. J Histochem Cytochem. 2012 February;60(2):97-109.
4.
Figure 5.

Figure 5. From: Three-Dimensional Microstructural Changes in Murine Abdominal Aortic Aneurysms Quantified Using Immunofluorescent Array Tomography.

Structural analysis: elastin thickness and non-adventitial wall thickness. (A) Elastin thickness decreased across all time points in the elastase group. The overall decrease in elastin thickness from day 0 to day 28 was larger in the elastase group (48.9%) than in the heat-inactivated elastase group (3.8%). (B) Non-adventitial wall thickness increased over time, with the largest increase (66.4%) occurring from day 7 to day 28 in the elastase group. Mean ± SEM, N=20, n=4 per group.

Sanaz Saatchi, et al. J Histochem Cytochem. 2012 February;60(2):97-109.
5.
Figure 4.

Figure 4. From: Three-Dimensional Microstructural Changes in Murine Abdominal Aortic Aneurysms Quantified Using Immunofluorescent Array Tomography.

Global analysis: volume fractions of elastin, smooth muscle cell actin (SMCA), and collagen type I. (A) A significant decrease in elastin volume fraction at day 7 and day 28 relative to day 0 was observed in the elastase group only. (B) SMCA volume fraction decreased significantly from day 0 to day 7 and returned at day 28, although still significantly suppressed relative to day 0, in both groups. (C) The largest decrease in adventitial collagen type I volume fraction occurred from day 0 to day 7 and remained significantly suppressed at day 28, relative to day 0, in both groups. Mean ± SEM, N=20, n=4 per group.

Sanaz Saatchi, et al. J Histochem Cytochem. 2012 February;60(2):97-109.
6.
Figure 3.

Figure 3. From: Three-Dimensional Microstructural Changes in Murine Abdominal Aortic Aneurysms Quantified Using Immunofluorescent Array Tomography.

Histology and immunohistochemistry. Histological and immunohistochemical characterization of elastin (A), smooth muscle cell actin (SMCA) (B), and adventitial collagen type I (C) validated immunofluorescent array tomography (IAT) findings. (A) Elastic van Gieson (EVG) results depict the loss of elastin thickness and organization and the increase in non-adventitial wall thickness that occurred during aneurysm development in the elastase group. Elastin lamellae structure and organization in the heat-inactivated elastase group were relatively constant. Arrows highlight elastin stained by EVG. (B) SMCA content changed dynamically with a decrease from day 0 to day 7 and subsequent increase from day 7 to day 28 in both the elastase and heat-inactivated elastase groups. Arrows highlight regions with positive staining for SMCA. (C) In both the elastase and heat-inactivated elastase groups, adventitial collagen type I content at days 7 and 28 decreased relative to day 0. Arrows highlight areas with positive staining for collagen type I. Orientation of all images: lumen at top and adventitia at bottom of image. Images taken at 63× magnification with scale bar representing 25 µm.

Sanaz Saatchi, et al. J Histochem Cytochem. 2012 February;60(2):97-109.
7.
Figure 2.

Figure 2. From: Three-Dimensional Microstructural Changes in Murine Abdominal Aortic Aneurysms Quantified Using Immunofluorescent Array Tomography.

Microstructural and cellular changes during aneurysm development. Three-dimensional volume renderings (x-y cross-sectional view) produced by immunofluorescent array tomography (IAT) enabled temporal investigation into microstructural, organizational, and cellular remodeling during aneurysm development. In the elastase group, elastin degradation was described by the loss of elastin thickness, density, and organization, whereas elastin fragmentation and non-adventitial wall thickness increased over the 28-day time course. Dynamic changes in smooth muscle cells (SMCs) were observed with a dramatic loss of smooth muscle cell actin (SMCA) at day 7 and a return in suppressed amounts at day 28. Adventitial collagen type I degradation was also prevalent over the 28-day time course. In the heat-inactivated elastase group, minimal changes in elastin were observed as elastin lamellae thickness, density, and organization remained relatively constant across time. Similar trends as those seen in the elastase group, specifically related to the decrease and return of SMCA and loss of adventitial collagen type I, were observed in the heat-inactivated elastase group. Top panel: 10× magnification; bottom panels: 63× magnification. Scale bar represents 10 µm, three-dimensional renderings with average total volume size of 136 µm (x), 104 µm (y), and 4.6 µm (z).

Sanaz Saatchi, et al. J Histochem Cytochem. 2012 February;60(2):97-109.

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