Figure 1.

Figure 1. From: Detection of BRAF mutations in patients with hairy cell leukemia and related lymphoproliferative disorders.

Pathological features of patient with BRAF K601E mutated splenic marginal zone lymphoma (SMZL) compared with hairy cell leukemia (HCL). (A) Peripheral blood showing marked lymphocytosis with small mature forms with occasional nuclear clefts and cytoplasmic projections (x200, Romanowsky). (B) Bone marrow biopsy showing both nodular and interstitial infiltrate of CD20+ lymphocytes (x50, CD20). (C) Strong nuclear staining for p53 in the majority of lymphocytes (x100, p53). (D) A representative metaphase from the neoplastic clone. Karyotype: 39-46,XY,del(2)(p10),-10,-14,+mar1,+mar2[cp7]/45-46,idem,del(7)(q21q31)[cp7]/46,XY[14]. (E) Flow cytometry (gated on lymphocytes) showing heterogenous CD11c expression, negative CD103 expression and weak CD25 expression. (F) Sequencing data from a section of reverse strand showing an A to G substitution at nucleotide position 1801 (arrow) in exon 15 of BRAF. (G) Typical hairy cell lymphocytes with oval nuclei and cytoplasmic projections (x400). (H) Flow cytometry (gated on lymphocytes) showing expression of CD20, CD11c, CD103 and CD25. (I) Sequencing data from a section of reverse strand showing a T to A substitution at nucleotide position 1799 (arrow) in exon 15 of BRAF.

Piers A. Blombery, et al. Haematologica. 2012 May;97(5):780-783.

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