We are sorry, but NCBI web applications do not support your browser and may not function properly. More information

Results: 5

1.
Figure 3.

Figure 3. From: MicroRNA profiling of follicular lymphoma identifies microRNAs related to cell proliferation and tumor response.

BCL2 is over-expressed in FL tumor cells. The relative mRNA counts of BCL2 were obtained from the NanoString nCounter assay. Lines indicate median values. P=0.02, 16.3-fold difference in BCL2 expression. FL: follicular lymphoma; FH: follicular hyperplasia.

Weixin Wang, et al. Haematologica. 2012 April;97(4):586-594.
2.
Figure 2.

Figure 2. From: MicroRNA profiling of follicular lymphoma identifies microRNAs related to cell proliferation and tumor response.

Heatmap of 28 genes differentially expressed in FL. Values used are normalized relative mRNA counts scaled to between 0 and 100. Nineteen FL tumor samples (17 pre-treatment biopsies, and 2 post-treatment; see text for details) and five FH B-cell samples are shown. FL: follicular lymphoma; FH: follicular hyperplasia; CR: complete response; PR: partial response; PD: progressive disease; NT: no treatment; PT: post-treatment.

Weixin Wang, et al. Haematologica. 2012 April;97(4):586-594.
3.
Figure 1.

Figure 1. From: MicroRNA profiling of follicular lymphoma identifies microRNAs related to cell proliferation and tumor response.

Heatmap of 44 miRNA differentially expressed in FL forming a unique FL signature. Signal intensity values used are values normalized by control gene probes and log2-transformed. Sixteen pre-treatment FL tumor cell samples and seven FH B-cell samples are shown in the heatmap. Response to chemotherapy is indicated for 14 patients who received PACE chemotherapy (see text). FL, follicular lymphoma; FH, follicular hyperplasia; CR, complete response; PR, partial response; PD, progressive disease; NT, no treatment.

Weixin Wang, et al. Haematologica. 2012 April;97(4):586-594.
4.
Figure 5.

Figure 5. From: MicroRNA profiling of follicular lymphoma identifies microRNAs related to cell proliferation and tumor response.

SOCS2 is regulated by miR-194 affecting tumor cell growth. (A), SOCS2 and miR-194 expression in patients’ FL tumor cells and control FH B cells. Relative signal intensity of miR-194 was obtained from a microRNA array and relative mRNA counts of SOCS2 were obtained from a NanoString nCounter assay. Lines indicate median values. P=0.02, 5.8-fold for miR-194; and P=0.04, 2.9-fold for SOCS2. FL, follicular lymphoma; FH, follicular hyperplasia. (B), The predicted conserved miR binding sites in 3′ untranslated (3′ UTR) of SOCS2 mRNA. The matched putative miRNA binding sequence in 3′ UTR of SOCS2 mRNA and miRNA seed sequence are in bold and underlined. (C) and (D), SOCS2 expression is regulated by miR-194 affecting tumor cell growth. OCI-Ly8 cells were transfected with miR-194 mimic oligonucleotides; the changes in SOCS2 mRNA level at 24, 36 and 48 h after transfection were determined by TaqMan quantitative real-time PCR assay. Mean and standard deviation of triplicate experiments are shown (C). Total live cells were counted 24 and 48 h after transfection using a Scepter hand-held automated cell counter and by trypan blue staining. Mean and standard deviation of triplicate counts are shown (D).

Weixin Wang, et al. Haematologica. 2012 April;97(4):586-594.
5.
Figure 4.

Figure 4. From: MicroRNA profiling of follicular lymphoma identifies microRNAs related to cell proliferation and tumor response.

CDKN1A is regulated by miR-20a and miR-20b affecting tumor cell growth. (A) CDKN1A, miR-20a and miR-20b expression in patients’ FL tumor cells and control FH B cells. Relative signal intensities of miR-20a and 20b were obtained from a microRNA array; and relative mRNA counts of CDKN1A were obtained from the NanoString nCounter assay. Lines indicate median values. P=0.02, greater than 9-fold difference for miR-20a; P=0.02, greater than 7-fold difference for miR-20b; and P=0.03, 11.8-fold difference in CDKN1A expression. FL, follicular lymphoma; FH, follicular hyperplasia. (B) The predicted conserved miRNA binding sites of miR-20a and miR-20b in the 3′ untranslated region (UTR) of CDKN1A mRNA. The matched putative miRNA binding sequence in 3′UTR of CDKN1A mRNA and miRNA seed sequence are in bold and underlined. (C) and (D), CDKN1A expression is regulated by miR-20a and miR-20b affecting tumor cell growth. OCI-Ly8 cells were separately transfected with miR-20a and-20b mimic oligonucleotides, the changes in CDKN1A/p21 mRNA level at 24, 36 and 48 h after transfection were determined by TaqMan quantitative real-time PCR assay. Mean and standard deviation of triplicate experiments are shown (C). Total live cells were counted 24 and 48 h after transfection using a Scepter hand-held automated cell counter and by trypan blue staining. Mean and standard deviation of triplicate counts are shown (D).

Weixin Wang, et al. Haematologica. 2012 April;97(4):586-594.

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk