Results: 4

1.
FIGURE 4

FIGURE 4. From: 124I-huA33 Antibody Uptake Is Driven by A33 Antigen Concentration in Tissues from Colorectal Cancer Patients Imaged by Immuno-PET.

Scatterplot of tissue %ID/mL estimated using DAR vs. well counter measurements (%ID/g) of intact tissue samples. Regression equation, y = 0.81 (SE, 0.12)x + 0.004 (SE, 0.002) (n = 29; r2 = 0.62).

Joseph A. O’Donoghue, et al. J Nucl Med. ;52(12):1878-1885.
2.
FIGURE 2

FIGURE 2. From: 124I-huA33 Antibody Uptake Is Driven by A33 Antigen Concentration in Tissues from Colorectal Cancer Patients Imaged by Immuno-PET.

(A–B) 18F-FDG PET/CT scan acquired immediately before administration of 124I-huA33 shows pathologic uptake in lesion in transverse colon near hepatic flexure together with normal physiologic accumulation. (C–D) PET/CT scan acquired 182 h after administration of 140 MBq (3.8 mCi) of 124I-huA33 shows focal uptake in tumor (compare B and D) and throughout normal intestine. (E–G) Contiguously aligned sections of tumor visualized in D assessed by 124I-DAR (E), hematoxylin and eosin staining (F), and immunohistochemical staining (G) for A33 antigen. 124I-huA33 localized only in small (~1 mm in dimension) regions of viable antigen-positive tumor and not in antigen-negative stroma. Scale bars = 2 mm.

Joseph A. O’Donoghue, et al. J Nucl Med. ;52(12):1878-1885.
3.
FIGURE 3

FIGURE 3. From: 124I-huA33 Antibody Uptake Is Driven by A33 Antigen Concentration in Tissues from Colorectal Cancer Patients Imaged by Immuno-PET.

(A) Example of A33 saturation binding curve for membranes derived from surgically removed tumor sample together with Scatchard transformed data plot (insert). Mean Kd ± SE, 0.31 ± 0.02 nM; mean Bmax ± SE, 1.07 ± 0.02 pmol/mg. (B) Scatterplot of A33 antigen density for membranes derived from tumor homogenates vs. 124I-huA33 uptake as determined by well counter measurements. Predicted 95% confidence limits are shown as dashed lines. Regression equation, y = 59 (SE, 7)x + 0.3 (SE, 0.1) (n = 26; r2 = 0.75). (C) Scatterplot of A33 antigen density for membranes derived from normal colon homogenates vs. 124I-huA33 uptake determined by well counter measurements. Regression equation, y = 97 (SE, 25)x + 0.09 (SE, 0.1) (n = 25; r2 = 0.40).

Joseph A. O’Donoghue, et al. J Nucl Med. ;52(12):1878-1885.
4.
FIGURE 1

FIGURE 1. From: 124I-huA33 Antibody Uptake Is Driven by A33 Antigen Concentration in Tissues from Colorectal Cancer Patients Imaged by Immuno-PET.

(A–B) 18F-FDG PET/CT scan acquired immediately before administration of 124I-huA33: coronal maximum-intensity-projection image (A) and transaxial PET/CT slice (B) through line of A. Images show pathologic uptake in primary (splenic flexure of colon) and metastatic (liver) lesions together with normal physiologic accumulation. (C–D) Clinical PET/CT scan acquired 162 h after administration of 395 MBq (10.7 mCi) of 124I-huA33: coronal maximum-intensity-projection (C) and transaxial PET/CT slice (D) through line of C. Intense focal uptake is visible in primary and metastatic tumor together with significant uptake throughout normal intestine. (E–J) DAR (E–G) and hematoxylin and eosin–stained (H–J) tissue sections corresponding to features observed in clinical image: primary tumor (E and H), normal colon (F and I), and normal liver (G and J). In tumor section, 124I-huA33 is localized primarily in regions of viable tumor cells with little stromal uptake. Normal colonic mucosa has high and uniform uptake. Little to no uptake is present in normal liver. Scale bars = 2 mm.

Joseph A. O’Donoghue, et al. J Nucl Med. ;52(12):1878-1885.

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