We are sorry, but NCBI web applications do not support your browser and may not function properly. More information

Results: 3

1.
FIGURE 2

FIGURE 2. From: Co-staining for Keratins 8/18 plus Ubiquitin Improves Detection of Hepatocyte Injury in Nonalcoholic Fatty Liver Disease.

Contingency plot analyses comparing H&E scoring parameters with IHC detection of hepatocellular injury. (A) The H&E scoring of ballooned hepatocytes is compared to the IHC analysis of KBH, (B) the H&E scoring of MDBs is compared to the IHC analysis of Ub deposits, (C) the H&E diagnosis of SH is compared to the IHC analysis of KBH, (D) the H&E diagnosis of SH is compared to the IHC analysis of Ub deposits, and (E) the H&E diagnosis of SH is compared to the IHC analysis of KH.

Cynthia D Guy, et al. Hum Pathol. ;43(6):790-800.
2.
FIGURE 3

FIGURE 3. From: Co-staining for Keratins 8/18 plus Ubiquitin Improves Detection of Hepatocyte Injury in Nonalcoholic Fatty Liver Disease.

Box plot graph with HOMA-IR (mg/dl*µU/ml) shown on the vertical axis and ‘None’ (0 per 10 HPFs), ‘Few’ (>0 and <10 per hpfs) and ‘Many’ (≥10 per hpfs) ubiquitin particles, KBH, and KH on the horizontal axis. The boxes represent the median (middle line), 25th percentile (bottom line of the box), and 75th percentile (top line of the box). The lowest and highest datum within 1.5 inter-quartile ranges for each category is represented by the ends of the whiskers. Median values of ‘None’, ‘Few’ and ‘Many’ with p-values were 3.8, 5.8, and 7.6 for ubiquitin particles (p=0.08), 3.6, 9.2, and 8.4 for KBH (p=0.02), and 3.8, 6.5, and 7.6(p=0.15) for KH.

Cynthia D Guy, et al. Hum Pathol. ;43(6):790-800.
3.
FIGURE 1

FIGURE 1. From: Co-staining for Keratins 8/18 plus Ubiquitin Improves Detection of Hepatocyte Injury in Nonalcoholic Fatty Liver Disease.

H&E and IHC stained sections showing hepatocellular injury. (A) An H&E stained section shows easily identified enlarged, rounded, swollen-appearing ballooned hepatocytes (arrow heads) with cytoplasmic clearing and reticulation and perinuclear Mallory-Denk bodies. (B) A Masson trichrome stained section shows numerous ballooned hepatocytes (arrow heads) with associated fibrosis. (C) K8/18 plus ubiquitin double IHC stained section shows several enlarged keratin negative ballooned hepatocytes (KBH) (arrow heads). The red-chromagen tagged ubiquitin antibody highlights Mallory-Denk bodies. The adjacent normal size hepatocytes show the usual homogeneous cytoplasmic staining with the brown chromagen-tagged K8/18 antibody. (D) Many normal size hepatocytes show complete loss of K8/18 staining (KH) (arrows). These KH also contain Mallory-Denk bodies. (E and F) Typical examples of foci of hepatocellular injury as seen with the double IHC stain. KBH (arrow heads) and KH (arrows) are often co-localized and adjacent to or intermixed with fibrosis. Many ubiquitinated protein aggregates are perinuclear, however, extracellular ubiquitin deposits (asterisks) can often also be identified. (G) In some cases, K8/18 staining was faint and patchy, however, KBH (arrow heads) and KH (arrows) can still be identified. (H1–H2) H1shows an H&E stained section of a somewhat linear area of fibrosis in which ballooned hepatocytes are not detected. H2 shows the double IHC stained section of the same focus; hepatocytes with loss of keratin staining and ubiquitin deposits are easily seen.

Cynthia D Guy, et al. Hum Pathol. ;43(6):790-800.

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk