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1.
Figure 9.

Figure 9. From: Net Intestinal Transport of Oxalate Reflects Passive Absorption and SLC26A6-mediated Secretion.

Proposed model of epithelial oxalate transport. Oxalate absorption is largely passive and paracellular across the tight junction. Oxalate then is back-secreted by a transcellular route requiring apical membrane SLC26A6.

Felix Knauf, et al. J Am Soc Nephrol. 2011 December;22(12):2247-2255.
2.
Figure 4.

Figure 4. From: Net Intestinal Transport of Oxalate Reflects Passive Absorption and SLC26A6-mediated Secretion.

A lumen-negative electrical gradient enhances oxalate absorption. We measured absorptive fluxes of [14C]oxalate and [3H]mannitol in the absence and presence of a lumen negative-electrical gradient. The data are expressed as the means ± SEM (n = 8). *P < 0.05 versus oxalate absorption in the absence of an electrical gradient.

Felix Knauf, et al. J Am Soc Nephrol. 2011 December;22(12):2247-2255.
3.
Figure 2.

Figure 2. From: Net Intestinal Transport of Oxalate Reflects Passive Absorption and SLC26A6-mediated Secretion.

Lack of effect of the anion inhibitor DIDS on oxalate absorption. We examined the effect of DIDS on [14C]oxalate absorption and secretion in the duodenum. The data are expressed as the means ± SEM (n = 4). *P < 0.05 versus oxalate secretory flux in absence of DIDS.

Felix Knauf, et al. J Am Soc Nephrol. 2011 December;22(12):2247-2255.
4.
Figure 3.

Figure 3. From: Net Intestinal Transport of Oxalate Reflects Passive Absorption and SLC26A6-mediated Secretion.

Lack of effect of unlabeled oxalate on [14C]oxalate absorption. We examined the effect of 0.2 mM unlabeled oxalate on absorption and secretion of 2 μM [14C]oxalate absorption and secretion in the duodenum. The data are expressed as the means ± SEM (n = 4). *P < 0.05 versus oxalate secretory flux in absence of unlabeled oxalate.

Felix Knauf, et al. J Am Soc Nephrol. 2011 December;22(12):2247-2255.
5.
Figure 5.

Figure 5. From: Net Intestinal Transport of Oxalate Reflects Passive Absorption and SLC26A6-mediated Secretion.

Oxalate and mannitol fluxes vary in parallel in different segments of the intestine. We measured absorptive fluxes of [14C]oxalate and [3H]mannitol along different segments of intestine of Slc26a6-null mice: duodenum, jejunum, ileum, proximal colon (P. colon), and distal colon (D. colon). The data are expressed as the means ± SEM (n = 4).

Felix Knauf, et al. J Am Soc Nephrol. 2011 December;22(12):2247-2255.
6.
Figure 1.

Figure 1. From: Net Intestinal Transport of Oxalate Reflects Passive Absorption and SLC26A6-mediated Secretion.

In the absence of SLC26A6, oxalate transport is largely passive and paracellular. Transepithelial absorption and secretion of [14C]oxalate were measured simultaneously with fluxes of [3H]mannitol across duodenum of wild-type and Slc26a6-null mice. The data are expressed as the means ± SEM (n = 8). *P < 0.05 versus oxalate absorptive flux.

Felix Knauf, et al. J Am Soc Nephrol. 2011 December;22(12):2247-2255.
7.
Figure 7.

Figure 7. From: Net Intestinal Transport of Oxalate Reflects Passive Absorption and SLC26A6-mediated Secretion.

Permeability of oxalate and mannitol increased in parallel with knockdown of ZO-1. Unidirectional absorptive fluxes of [14C]oxalate and [3H]mannitol were measured in control and ZO-1 knockdown cells (ZO-1 KD). Immunoblot confirming ZO-1 knockdown is shown in inset. The data are expressed as the means ± SEM (n = 4). *P < 0.05 of oxalate and mannitol permeability in ZO-1 knockdown cells versus oxalate and mannitol permeability, respectively, in control cells.

Felix Knauf, et al. J Am Soc Nephrol. 2011 December;22(12):2247-2255.
8.
Figure 8.

Figure 8. From: Net Intestinal Transport of Oxalate Reflects Passive Absorption and SLC26A6-mediated Secretion.

Lack of effect of medium magnesium concentration on oxalate absorption. We examined the effect of increasing MgCl2 in the medium from 1.2 to 10 mM on oxalate absorption and secretion in the duodenum. The data are expressed as the means ± SEM (n = 4). *P < 0.05 versus oxalate secretory flux in presence of 1.2 mM magnesium.

Felix Knauf, et al. J Am Soc Nephrol. 2011 December;22(12):2247-2255.
9.
Figure 6.

Figure 6. From: Net Intestinal Transport of Oxalate Reflects Passive Absorption and SLC26A6-mediated Secretion.

Lack of effect of claudin-10a expression on oxalate and mannitol permeability. (A) We measured dilution potentials (mV) across MDCK II monolayers following a 120 to 60 mM apical NaCl dilution without and with induction of claudin-10a expression. Immunoblot confirming claudin-10a induction is shown in the inset. The data are expressed as the means ± SEM (n = 4). *P < 0.05 versus uninduced cells. (B) Despite an effect of claudin-10a on anion permeability as measured by dilution potential, oxalate and mannitol permeability remained unaffected by induction of claudin-10a. The data are expressed as the means ± SEM (n = 4).

Felix Knauf, et al. J Am Soc Nephrol. 2011 December;22(12):2247-2255.

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