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Results: 5

1.
Figure 3

Figure 3. Structure of the BmHSP70 promoter. From: Analysis of Transcriptional Regulation of Tetracycline Responsive Genes in Brugia malayi.

Areas highlighted in grey indicate sequences which, when mutated, resulted in the loss of 50–90% of basal promoter activity, while sequences highlighted in black represent those which, when mutated, resulted in the loss of >90% of basal promoter activity [20]. The functional HSE is indicated by a dotted box, and the TATAA-box like element by underlining.

Canhui Liu, et al. Mol Biochem Parasitol. ;180(2):106-111.
2.
Figure 2

Figure 2. Reporter gene activity in embryos transfected with candidate tetracycline responsive promoters in the presence or absence of tetracycline. From: Analysis of Transcriptional Regulation of Tetracycline Responsive Genes in Brugia malayi.

Embryos were transfected with promoter constructs, cultured in the presence or absence of tetracycline and assayed for reporter gene activity as described in Materials and Methods. Columns indicate the mean, and error bars the standard deviations, of reporter gene activity in six independent transfections. Asterisks indicate promoters whose activity was significantly up-regulated in the presence of tetracycline (p < 0.01).

Canhui Liu, et al. Mol Biochem Parasitol. ;180(2):106-111.
3.
Figure 1

Figure 1. Reporter gene activity in embryos transiently transfected with BmMFSP (−1194 to −1)/ren in the presence or absence of tetracycline. From: Analysis of Transcriptional Regulation of Tetracycline Responsive Genes in Brugia malayi.

Embryos were transiently transfected as described in Materials and Methods, cultured for 48 hours in the presence or absence of 40 μg ml−1 tetracycline and assayed for renilla luciferase reporter gene activity. Data are expressed as relative light units (RLU) mg−1 parasite protein.

Canhui Liu, et al. Mol Biochem Parasitol. ;180(2):106-111.
4.
Figure 4

Figure 4. Reporter gene activity in embryos transfected with 30nt substitution mutants of the BmHSP70 promoter in the presence or absence of tetracycline. From: Analysis of Transcriptional Regulation of Tetracycline Responsive Genes in Brugia malayi.

Embryos were transfected with promoter constructs, cultured in the presence or absence of tetracycline and assayed for reporter gene activity as described in Materials and Methods. Panel A: Reporter gene activity of promoter constructs in the presence or absence of tetracycline. Columns indicate the mean and error bars the standard deviations of reporter gene activity in six independent transfections. Panel B: Extent of up-regulation of reporter activity in the presence of tetracycline. Asterisks indicate constructs whose activity was significantly up-regulated in the presence of tetracycline (p < 0.01).

Canhui Liu, et al. Mol Biochem Parasitol. ;180(2):106-111.
5.
Figure 5

Figure 5. Reporter gene activity in embryos transfected with small substitution mutants of the BmHSP70 promoter in the presence or absence of tetracycline. From: Analysis of Transcriptional Regulation of Tetracycline Responsive Genes in Brugia malayi.

Embryos were transfected with promoter constructs, cultured in the presence or absence of tetracycline and assayed for reporter gene activity as described in Materials and Methods. Panel A: Reporter gene activity of promoter constructs in the presence or absence of tetracycline. Columns indicate the mean and error bars the standard deviations of reporter gene activity in six independent transfections. Panel B: Extent of up-regulation of reporter activity in the presence of tetracycline. Asterisks indicate constructs whose activity was significantly up-regulated in the presence of tetracycline (p < 0.01).

Canhui Liu, et al. Mol Biochem Parasitol. ;180(2):106-111.

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