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1.
Figure 1

Figure 1. Skeletal unloading affects proliferation of BMOp cells in response to IGF-I and PDGF in vitro. From: Skeletal unloading induced insulin-like growth factor-I (IGF-I) non-responsiveness is not shared by platelet-derived growth factor: the selective role of integrins in IGF-I signaling.

BMOp cells from loaded and unloaded bones were incubated with IGF-I (A) or PDGF (B) for 24 hrs at day 7 in culture. During the last 4 hours, the cultures were labeled with BrdU and absorbance quantifies the BrdU incorporation and proliferation. Means ± SD, n = 3. a p < 0.05 vs. Loaded + IGF-I.

Roger K Long, et al. J Bone Miner Res. ;26(12):2948-2958.
2.
Figure 2

Figure 2. Effect of skeletal unloading on growth factor stimulated phosphorylation of ERK1/2. From: Skeletal unloading induced insulin-like growth factor-I (IGF-I) non-responsiveness is not shared by platelet-derived growth factor: the selective role of integrins in IGF-I signaling.

BMOp cells from loaded and unloaded bones were serum deprived at day 7 in culture, and then treated with IGF-I (A) or PDGF (B). Representative immunoblots illustrate skeletal unloading induced impairment of IGF-I stimulated ERK1/2 phosphorylation. Relative signal intensities of the ratio of phosphorylated to total ERK1/2 were evaluated. Means ± SD, n = 3. a p < 0.05 vs. Loaded + IGF-I.

Roger K Long, et al. J Bone Miner Res. ;26(12):2948-2958.
3.
Figure 4

Figure 4. Effect of echistatin on the activation of the PDGF and IGF-I receptors. From: Skeletal unloading induced insulin-like growth factor-I (IGF-I) non-responsiveness is not shared by platelet-derived growth factor: the selective role of integrins in IGF-I signaling.

BMOp cells from loaded bones were serum deprived at day 7 in culture, and then treated with IGF-I (A) or PDGF (B). Twelve hours prior, echistatin (100nM) or vehicle was added. Representative immunoblots illustrate echistatin-induced impairment of IGF-I receptor phosphorylation. Relative signal intensities of the ratio of phosphorylated to total IGF-I and PDGF receptors were evaluated. Means ± SD, n = 3. a p < 0.05 vs. Control.

Roger K Long, et al. J Bone Miner Res. ;26(12):2948-2958.
4.
Figure 6

Figure 6. Recovery of IGF-I signaling in BMOp cells from unloaded bone followed longitudinally. From: Skeletal unloading induced insulin-like growth factor-I (IGF-I) non-responsiveness is not shared by platelet-derived growth factor: the selective role of integrins in IGF-I signaling.

BMOp cells from loaded and unloaded bones were grown in culture over 21 days. At days 14 and 21, cultures were serum deprived and then treated with IGF-I. Representative immunoblots illustrate recovery of ligand induced IGF-I receptor (A) and ERK1/2 (B) phosphorylation at 14 and 21 days in culture. Relative signal intensities of the ratio of phosphorylated to total IGF-I receptor and ERK1/2 were evaluated. Means ± SD, n = 4. There were no significant differences.

Roger K Long, et al. J Bone Miner Res. ;26(12):2948-2958.
5.
Figure 7

Figure 7. Effects of targeted integrin subunit knockdown on IGF-I signaling. From: Skeletal unloading induced insulin-like growth factor-I (IGF-I) non-responsiveness is not shared by platelet-derived growth factor: the selective role of integrins in IGF-I signaling.

(A) BMOp cells from loaded bones were treated with specific (siβ1 or siβ3) or non-targeting control (siCont) siRNA oligonucleotides on day 4 in culture. At day 7, cultures were serum deprived and then treated with IGF-I. RNA expression of β1 or β3 integrin subunits following targeted siRNA treatment was specifically and significantly reduced. Means ± SEM, n = 4, a p < 0.05 vs. siCont. (B) A representative immunoblot confirms the diminished expression of β1 and β3 subunit following siRNA treatment. (C) IGF-IR activation is impaired by β1 or β3 integrin subunit knockdown. (D) Relative signal intensities of the ratio of phosphorylated to total IGF-I receptor were evaluated. Means ± SEM, n = 3, a p < 0.05 vs. siCont.

Roger K Long, et al. J Bone Miner Res. ;26(12):2948-2958.
6.
Figure 3

Figure 3. Effect of skeletal unloading on the activation of PDGF and IGF-I receptors. From: Skeletal unloading induced insulin-like growth factor-I (IGF-I) non-responsiveness is not shared by platelet-derived growth factor: the selective role of integrins in IGF-I signaling.

BMOp cells from loaded and unloaded bones were serum deprived at day 7 in culture, and then treated with IGF-I (A) or PDGF (B). Representative immunoblots illustrate skeletal unloading induced impairment of IGF-I receptor phosphorylation. Relative signal intensities of the ratio of phosphorylated to total IGF-I or PDGF receptors were evaluated. Means ± SD, n = 3. a p < 0.05 vs. Loaded. (C) BMOp cells from loaded and unloaded bones were serum deprived at day 7 in culture, and then exposed to 25pM 125I-IGF and various doses of unlabeled IGF-I or des-IGF-I for 10 minutes. Data are means of triplicates. Error bars are not shown to simplify presentation, but in all cases enclosed an SD of less than 10% of the mean. These values are expressed as a percentage of maximal specific 125I-IGF binding that was comparable for BMOp cells isolated from loaded and unloaded bones.

Roger K Long, et al. J Bone Miner Res. ;26(12):2948-2958.
7.
Figure 5

Figure 5. Recovery of skeletal unloading induced reduction in expression of integrin subunits. From: Skeletal unloading induced insulin-like growth factor-I (IGF-I) non-responsiveness is not shared by platelet-derived growth factor: the selective role of integrins in IGF-I signaling.

The BMOp cells from loaded and unloaded bones were grown in culture. Total RNA was harvested at days 5, 7, 14, and 21 in culture and cell lysates collected at days 7, 14, and 21 in culture. A) Relative mRNA expression of the integrin subunits normalized to L19 of the unloaded BMOp cells as a percentage of that of loaded BMOp are displayed. Means ± SEM, of four independent BMOp cell pools at day 5, thirteen at day 7, six at day 14 and four at day 21. a p < 0.05, b p < 0.01, c p = 0.055. B) A representative immunoblot illustrates that expression of the β1 integrin subunit was significantly less than that in loaded BMOp cells after 7 days but not 14 or 21 days in culture. Relative signal intensities of the ratio of β1 integrin to actin were evaluated. Means ± SD, n = 3. a p < 0.05 vs. Loaded.

Roger K Long, et al. J Bone Miner Res. ;26(12):2948-2958.

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