Results: 3

1.
Figure 3

Figure 3. From: Cardiac-Specific Mutation of Clock Alters the Quantitative Measurements of Physical Activities without Changing Behavioral Circadian Rhythms.

The daily profiles of Bmal1, VGCCα1C, and VGCCα1D mRNA in CCM mice and their WT littermates. (A) There was no rhythmic expression of VGCCα1C mRNA in either WT or CCM mice. n = 6 for each time point. (B) The protein expression of VGCCα1C was not rhythmic in both WT and CCM mice. (C) The VGCCα1D mRNA at ZT 0 was significantly higher than ZT 6 and 18 in WT mice. The diurnal rhythm of VGCCα1D mRNA was abolished in CCM mice. n = 6 for each time point. (D) In WT mice, the protein expression of VGCCα1D peaked at ZT 6, which was significantly different than the protein level at ZT 0 and 18. The diurnal rhythm of VGCCα1D was abolished in CCM mice. (E) There was a diurnal rhythm of Bmal1 mRNA in both WT and CCM mice, but the rhythmic amplitude was smaller in CCM mice. *In WT mice, Bmal1 levels at ZT 0 and 18 were significantly higher than ZT 6 and 12. n = 3 for each time point.

Michael L. Ko, et al. J Biol Rhythms. ;26(5):412-422.
2.
Figure 2

Figure 2. From: Cardiac-Specific Mutation of Clock Alters the Quantitative Measurements of Physical Activities without Changing Behavioral Circadian Rhythms.

The daily rhythms of protein expression and phosphorylation states in CCM mice and their WT littermates. (A) The phosphorylation of p42/44 ERK was rhythmic in WT mice (n = 5), in which diphosphorylated ERK (pERK) peaked at ZT 0. In CCM mice (n = 5), pERK was no longer rhythmic. (B) The phosphorylation of p38 kinase was rhythmic in both WT (n = 3) and CCM mice (n = 3). *Phosphorylated p38 at ZT 12 was significantly higher than ZT 0, 6, and 18 in WT mice. #Phosphorylated p38 at ZT 12 was significantly higher than ZT 6 in CCM mice. (C) The phosphorylation of AKT at thr308 (pAKTthr308) peaked at ZT 6 in WT mice (n = 3), while the diurnal rhythm of pAKTthr308 was dampened in CCM mouse (n = 3) hearts. (D) In WT mouse hearts (n = 4), the phosphorylation of GSK3β (pGSK) was significantly higher at ZT 18 compared to ZT 0 and 6, while there was no diurnal rhythm of pGSK in CCM mice (n = 3).

Michael L. Ko, et al. J Biol Rhythms. ;26(5):412-422.
3.
Figure 1

Figure 1. From: Cardiac-Specific Mutation of Clock Alters the Quantitative Measurements of Physical Activities without Changing Behavioral Circadian Rhythms.

Wheel-running locomotor activity rhythms in CCM and WT mice. (A and B) Double-plotted wheel-running actograms from a WT mouse (A) housed in LD (top panel) and in DD (lower panel) and from a CCM mouse (B) housed in LD (top panel) and in DD (lower panel). When mice were in LD cycles, the lights were on at 0600 h and off at 1800 h. (C) The WT mice had significantly higher total numbers of wheel revolutions (total activity counts) compared to CCM littermates. (D) There was no difference between WT and CCM mice in total number of bouts per day (bouts per day). (E) The CCM mice had significantly lower length of time for each bout on average (average bout length in minutes) compared to WT mice. (F) The WT mice had significantly higher counts of wheel revolutions for each bout (average counts/bout) compared to CCM mice. WT: n = 21; CCM: n = 17.

Michael L. Ko, et al. J Biol Rhythms. ;26(5):412-422.

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