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1.
Figure 2

Figure 2. From: Proteomic profiling of the mesenteric lymph after hemorrhagic shock: Differential gel electrophoresis and mass spectrometry analysis.

Analytical DIGE Image (single animal) of rat mesenteric lymph. Merged image of the Cy 5 and Cy3 scans from the analytical gel used for additional spot picking and protein identifications. The pH and MW range are the same as in Figure 1. 50 μg of pre and post-shock was used including a pooled internal standard labeled with Cy 2 that is not shown. Proteins identified are labeled with numbers that correspond to identification (Additional File 2, Table 2).

Ashley Zurawel, et al. Clin Proteomics. 2011;8(1):1-1.
2.
Figure 3

Figure 3. From: Proteomic profiling of the mesenteric lymph after hemorrhagic shock: Differential gel electrophoresis and mass spectrometry analysis.

Western blots of mesenteric lymph before and after shock. The expression of β-actin, major urinary protein (MUP) and apolipoprotein E (Apo E) in pre-shock and post shock mesenteric lymph. Lanes 1 and 2 are pre-shock meserteric lymph form two animals; the lanes 1' and 2' are from post-shock mesenteric lymph from the same animals. Each lane contained 20 μg total protein. Ponceau S staining (lower image) of the membrane was used to evaluate protein loading and transfer.

Ashley Zurawel, et al. Clin Proteomics. 2011;8(1):1-1.
3.
Figure 1

Figure 1. From: Proteomic profiling of the mesenteric lymph after hemorrhagic shock: Differential gel electrophoresis and mass spectrometry analysis.

Image of rat mesenteric lymph (collected with EDTA) separated by 2D gel electrophoresis. Image of the Sypro stained preparative gel. The horizontal axis represent pH, here ranging from 3 on the left to 10 on the right, and the vertical axis represent molecular weight. A total of 500 μg of each pre and post-shock lymph, representing protein precipitated from a pool from three equally represented biological variants was focused onto a 24 cm Immobiline DryStrip, and then separated by molecular weight down the gel. Identifications made by DIGE and mass spectrometry analysis are marked by numbers that correspond to proteins listed in Table 1. (Attached File).

Ashley Zurawel, et al. Clin Proteomics. 2011;8(1):1-1.

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