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1.
FIG. 1.

FIG. 1. From: In Vitro Cartilage Tissue Engineering Using Adipose-Derived Extracellular Matrix Scaffolds Seeded with Adipose-Derived Stem Cells.

Macroscopic (A) and scanning electron microscopy (B) images of an hECM gel-like scaffold. Scale bars represent 100 μm. hECM, human extracellular matrix. Color images available online at www.liebertonline.com/tea

Ji Suk Choi, et al. Tissue Eng Part A. 2012 January;18(1-2):80-92.
2.
FIG. 2.

FIG. 2. From: In Vitro Cartilage Tissue Engineering Using Adipose-Derived Extracellular Matrix Scaffolds Seeded with Adipose-Derived Stem Cells.

Quantification of growth factors extracted from hECM scaffolds. Three different types of extraction buffers (containing either 2 M sodium chloride, 2 M urea, or 0.5 M acetic acid) were used. Data are shown as means±standard deviations with significance at *p<0.01.

Ji Suk Choi, et al. Tissue Eng Part A. 2012 January;18(1-2):80-92.
3.
FIG. 7.

FIG. 7. From: In Vitro Cartilage Tissue Engineering Using Adipose-Derived Extracellular Matrix Scaffolds Seeded with Adipose-Derived Stem Cells.

DNA (A), protein (B), total collagen (C), and sGAG (D) contents in cell pellets (CP) and hASC/hECM composites (EC). CP (+) and EC (+), with addition of 10 ng/mL TGF-β1. CP (−) and EC (−), without TGF-β1. All contents were normalized to DNA content in each condition. Data are shown as means±standard deviations with significance at *,**,***p<0.01 between the groups. sGAG, sulfated glycosaminoglycan. Color images available online at www.liebertonline.com/tea

Ji Suk Choi, et al. Tissue Eng Part A. 2012 January;18(1-2):80-92.
4.
FIG. 5.

FIG. 5. From: In Vitro Cartilage Tissue Engineering Using Adipose-Derived Extracellular Matrix Scaffolds Seeded with Adipose-Derived Stem Cells.

Scanning electron microscopy micrographs of hASC/hECM composites on day 1 (A, B) and day 45 (C–F). Composites were maintained in chondrogenic medium without (A, C, E) or with TGF-β1 (B, D, F). Scale bars represent 20 μm (white bold line) and 10 μm (white dotted line).

Ji Suk Choi, et al. Tissue Eng Part A. 2012 January;18(1-2):80-92.
5.
FIG. 4.

FIG. 4. From: In Vitro Cartilage Tissue Engineering Using Adipose-Derived Extracellular Matrix Scaffolds Seeded with Adipose-Derived Stem Cells.

(A) Gross morphologies and (B) wet weights of hASC/hECM composites on day 5, 15, 30, and 45. Scales of a ruler represent 1 mm. EC (+), with addition of 10 ng/mL TGF-β1; EC (−), without TGF-β1. Data are shown as means±standard deviations (n=5) with significance at *,**p<0.05. TGF-β1, transforming growth factor beta1. Color images available online at www.liebertonline.com/tea

Ji Suk Choi, et al. Tissue Eng Part A. 2012 January;18(1-2):80-92.
6.
FIG. 6.

FIG. 6. From: In Vitro Cartilage Tissue Engineering Using Adipose-Derived Extracellular Matrix Scaffolds Seeded with Adipose-Derived Stem Cells.

Fluorescence micrographs of hASC/hECM composites on day 1 (A, C) and day 45 (B, D). Composites were maintained in chondrogenic medium without (A, B) or with TGF-β1 (C, D). Cells were stained by calcein-AM (green=live) and ethidium homodimer (red=dead). The white arrows indicate dead cells. Scale bars represent 200 μm (white bold line) and 1 mm (yellow). Color images available online at www.liebertonline.com/tea

Ji Suk Choi, et al. Tissue Eng Part A. 2012 January;18(1-2):80-92.
7.
FIG. 3.

FIG. 3. From: In Vitro Cartilage Tissue Engineering Using Adipose-Derived Extracellular Matrix Scaffolds Seeded with Adipose-Derived Stem Cells.

Flow cytometry histograms for mesenchymal stem cells markers (CD29, CD44, and CD105) were displayed on hASCs at passage 6. Each color line (red, orange, blue) indicates positive staining cells, whereas the purple area and black line indicate the isotype-matched monoclonal antibody control. hASC, human adipose-derived stem cell. Color images available online at www.liebertonline.com/tea

Ji Suk Choi, et al. Tissue Eng Part A. 2012 January;18(1-2):80-92.
8.
FIG. 9.

FIG. 9. From: In Vitro Cartilage Tissue Engineering Using Adipose-Derived Extracellular Matrix Scaffolds Seeded with Adipose-Derived Stem Cells.

Reverse-transcription-polymerase chain reaction analysis of gene expression for chondrogenic differentiation in hASC/hECM composites. hASC undifferentiated cells (A) were used as a negative control (lane 2). Each expressed gene was analyzed using human-origin primers designed for Sox-9, AGN, collagen type II α1 (Col IIα1), collagen type X (Col X α1), and collagen type XI α1 (Col XIα1). GAPDH was used for normalization.

Ji Suk Choi, et al. Tissue Eng Part A. 2012 January;18(1-2):80-92.
9.
FIG. 8.

FIG. 8. From: In Vitro Cartilage Tissue Engineering Using Adipose-Derived Extracellular Matrix Scaffolds Seeded with Adipose-Derived Stem Cells.

Histological and immunofluorescence characterization of hASC/hECM composites (n=5) after 45 days of in vitro culture. (A, C, E, G) EC (−) and (B, D, F, H) EC (+). Cell nuclei (black spot) and cytoplasm (pink) were stained by hematoxylin and eosin (A, B). Sulfated proteoglycans were identified via Safranin-O (dark purple; C, D), and Alcian blue (blue; E, F) staining. Collagen type II was stained by a monoclonal collagen type II-conjugated fluorescein isothiocyanate (green). Cell nuclei in the composites were counterstained using DAPI (blue) (G, H). Scale bars represent 1 mm (bold line) and 400 μm (dotted line). Color images available online at www.liebertonline.com/tea

Ji Suk Choi, et al. Tissue Eng Part A. 2012 January;18(1-2):80-92.

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