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Results: 3

1.
Figure 1

Figure 1. From: Genomic sequencing of colorectal adenocarcinomas identifies a recurrent VTI1A-TCF7L2 fusion.

DNA structural rearrangements and copy number alterations detected in the nine colorectal tumors displayed as CIRCOS plots33. Chromosomes are arranged circularly end-to-end with each chromosome’s cytobands marked in the outer ring. The inner ring displays copy number data inferred from whole-genome sequencing with blue indicating losses and red indicating gains. Within the circle, rearrangements are shown as arcs with intrachromosomal events in green and interchromosomal translocations in purple.

Adam J Bass, et al. Nat Genet. 2011 September 4;43(10):964-968.
2.
Figure 2

Figure 2. From: Genomic sequencing of colorectal adenocarcinomas identifies a recurrent VTI1A-TCF7L2 fusion.

Complex rearrangements between chromosome pairs in two colorectal carcinomas. The central portion of the figure contains copy-number profiles across all chromosomes with the chromosome identity labeled across the x axis and the scale for copy-number ratio (log2) depicted on the y axis of each plot. The upper plot shows the tumor CRC-4, and the lower plot shows the copy-number profile for CRC-6, with the black dots marking the copy-number ratio inferred along each locus across the genome. The upper inset boxes show detailed views of the copy numbers and rearrangements for chromosomes 8 (dark blue) and 20 (ochre) for CRC-4 with the centromere labeled as a purple circle. Rearrangements detected by dRanger are shown in green (intrachromosomal) and purple (interchromosomal). The lower inset boxes show detailed copy-number and rearrangement images for CRC-6, with inset boxes showing chromosome 5 (red) and 11 (gray), with lines marking positions of genomic rearrangements.

Adam J Bass, et al. Nat Genet. 2011 September 4;43(10):964-968.
3.
Figure 3

Figure 3. From: Genomic sequencing of colorectal adenocarcinomas identifies a recurrent VTI1A-TCF7L2 fusion.

Recurrent gene fusion between VTI1A and TCF7L2. (a) The upper schematic depicts the positions of exons (vertical lines) within VTI1A and TCF7L2, which reside adjacent to each other on chromosome 10. The blowup displays the locations of discordant paired-end reads found in tumor CRC-9 for which one read (labeled in blue) is in an intron of VTI1A and the other read (labeled in red) is in an intron of TCF7L2. (b) The upper schematic depicts the structure of the predicted fusion transcript generated by the fusion. The presence of the exact reads spanning the fusion of the two introns (marked by lightning bolt) is depicted in the inset with regions of the reads corresponding to original VTI1A intron in blue and those of TCF7L2 in red. (c) The protein domain structure of native VTI1A and TCF4-TCF7L2, including the two alternate C-terminal tails of TCF4, are shown. Below are the structures of the fusion protein encoded by the fusion of exon 3 of VTI1A to exon 4 of TCF7L2 identified in CRC-9. Two variants of the fusion are shown as data from the NCI-H508 cell line and reveal that variants encoding both the full length (E-tail) and shorter (B-tail) C termini are both expressed (data not shown). (d) Measurement of the relative expression of the VTI1A-TCF7L2 mRNA in NCI-H508 cells infected with one of two short hairpin RNA constructs targeting the fusion gene relative to expression in a cell infected with control vectors targeting GFP. (e) Anchorage-independent growth of the NCIH508 cell line, which expresses VTI1A-TCF7L2, and negative control DLD-1 colorectal adenocarcinoma cells following RNA-interference–mediated knockdown of VTI1A-TCF7L2 compared to control knockdown targeting GFP.

Adam J Bass, et al. Nat Genet. 2011 September 4;43(10):964-968.

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